NODAL

Gene Symbol NODAL
Entrez Gene 4838
Alt Symbol HTX5
Species Human
Gene Type protein-coding
Description nodal growth differentiation factor
Other Description nodal homolog
Swissprots Q96S42 Q8N4V3 Q2M3A5
Accessions BAB62524 EAW54403 Q96S42 BC033585 AAH33585 BC039861 BC104976 AAI04977 BC112025 AAI12026 NM_018055 NP_060525
Function Essential for mesoderm formation and axial patterning during embryonic development. {ECO:0000250}.
Subcellular Location Secreted {ECO:0000250}.
Top Pathways TGF-beta signaling pathway, Signaling pathways regulating pluripotency of stem cells

Nodal (A-9) - sc-377508 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nodal (A-9): sc-377508. Western blot analysis of Nodal expression in non- transfected (A) and human Nodal transfected (B) whole cell lysates.

Nodal (C-17) - sc-34838 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nodal (C-17): sc-34838. Western blot analysis of human recombinant Nodal fusion protein.

Nodal (G-10) - sc-373910 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nodal (G-10): sc-373910. Western blot analysis of Nodal expression in non-transfected (A) and human Nodal transfected (B) CMV whole cell lysates.

Nodal (H-110) - sc-28913 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Nodal (H-110): sc-28913. Western blot analysis of Nodal expression in HeLa (A), THP-1 (B) and MIA PaCa-2 (C) whole cell lysates.

Nodal (H-20) - sc-30511 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nodal (H-20): sc-30511. Western blot analysis of human recombinant Nodal fusion protein.

Nodal (P-15) - sc-34839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Nodal (WS65) - sc-81953 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Nodal (WS65): sc-81953. Western blot analysis of Nodal expression in HeLa whole cell lysate.

Anti-Nodal antibody - ab55676 from Abcam

Prices $398.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Nodal antibody (ab55676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.

Anti-Nodal antibody - ab39953 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab39953 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39953, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

Anti-Nodal antibody - ab99196 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Nodal antibody (ab99196) at 1 µg/ml + Human fetal thymus lysate at 10 µg

Anti-Nodal antibody - ab110162 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Human Brain, cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab110162 at a concentration of 3.75 µg/ml.

Anti-Nodal antibody [EP2058Y] - ab81287 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2058Y
Anti-Nodal antibody [EP2058Y] (ab81287) at 1/2000 dilution + Recombinant nodal protein at 10ngSecondaryHRP conjugated goat anti-rabbit at 1/2000 dilution

Anti-Nodal antibody [EPR2057] - ab109317 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2057
All lanes : Anti-Nodal antibody [EPR2057] (ab109317) at 1/1000 dilutionLane 1 : SH-SY5Y lysateLane 2 : PC12 lysateLysates/proteins at 10 µg per lane.

Anti-NODAL Antibody (aa279-289) - LS-C55181 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-NODAL Antibody (aa35-84) - LS-C111971 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NODAL antibody LS-C111971 Western blot of Fetal Thymus lysate.

Anti-NODAL Antibody (C-Terminus, clone EP2058Y) - LS-C105253 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2058Y
Western blot analysis on 10 ng of recombinant nodal protein using anti-Nodal antibody (LS-C105253), dilution 1:2000.

Anti-NODAL Antibody (C-Terminus) - LS-C116663 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NODAL antibody LS-C116663 Western blot of THP-1 cell lysate.

Anti-NODAL Antibody (aa200-347) - LS-C143596 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Nodal Antibody - WB analysis of NODAL in HeLa whole cell extracts.

Anti-NODAL Antibody (Internal, clone EPR2057) - LS-C138363 from LifeSpan Bioscience

Prices $480.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2057
Western blot analysis on (A) SH-SY5Y and (B) PC-12 cell lysates using anti-Nodal antibody.

Anti-NODAL Antibody (clone 5C3) - LS-C133350 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5C3
Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]

Anti-NODAL Antibody (aa242-253) IHC-plus™ - LS-B3955 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-NODAL antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3955 concentration 3.75 ug/ml.

Nodal Antibody - NBP1-71686 from Novus Biologicals

Prices $279.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Nodal Antibody [NBP1-71686] - Analysis of NODAL in HeLa whole cell extracts.

Nodal Antibody (5C3) - H00004838-M03 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5C3
Western Blot: Nodal Antibody (5C3) [H00004838-M03] - Analysis of NODAL expression in PC-12 (Cat # L012V1).

Nodal Antibody - AF1315 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal

Nodal Antibody (784410) - MAB3218 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 784410
Western blot shows lysates of undifferentiated or mesendoderm-differentiated BG01V human embryonic stem cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Nodal Monoclonal Antibody (Catalog # MAB3218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nodal at approximately 35-47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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