FOXO1

Gene Symbol	FOXO1
Entrez Gene	2308
Alt Symbol	FKH1, FKHR, FOXO1A
Species	Human
Gene Type	protein-coding
Description	forkhead box O1
Other Description	forkhead box protein O1|forkhead box protein O1A|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
Swissprots	Q6NSK6 Q5VYC7 O43523 Q12778
Accessions	AAC50342 AAC50343 EAX08627 Q12778 AF032885 AAC39591 AK310511 BC021981 AAH21981 BC070065 AAH70065 BT007455 AAP36123 BX648278 U02310 AAA03629 U36922 XM_011535008 XP_011533310 XM_011535009 XP_011533311 XM_011535010 XP_011533312 XR_941536 NM_002015 NP_002006
Function	Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and S
Subcellular Location	Cytoplasm {ECO:0000250|UniProtKB:Q9R1E0}. Nucleus {ECO:0000250|UniProtKB:Q9R1E0}. Note=Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells. In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway. Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation. Phosphorylation by NLK results in nuclear export. Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1. SGK1-mediated phosphorylation also results in nuclear translocation. Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide. Retained in the nucleus on methylation. {ECO:0000250}.
Tissue Specificity	Ubiquitous. {ECO:0000269|PubMed:9479491}.
Top Pathways	Insulin signaling pathway, Prostate cancer, AMPK signaling pathway, Glucagon signaling pathway, Transcriptional misregulation in cancer

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

SignalSilence ® FoxO1 siRNA I - 6242 from Cell Signaling Technology Category siRNA Prices $256.00 Sizes 300 µl (3 nmol) Species Reactivities Human

SignalSilence ® FoxO1 siRNA II - 6256 from Cell Signaling Technology Category siRNA Prices $256.00 Sizes 300 µl (3 nmol) Species Reactivities Human

FKHR CRISPR/Cas9 KO Plasmid (h) - sc-400137 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR HDR Plasmid (h) - sc-400137-HDR from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR Double Nickase Plasmid (h) - sc-400137-NIC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR Double Nickase Plasmid (h2) - sc-400137-NIC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR CRISPR Activation Plasmid (h) - sc-400137-ACT from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR CRISPR Activation Plasmid (h2) - sc-400137-ACT-2 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR Lentiviral Activation Particles (h) - sc-400137-LAC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR Lentiviral Activation Particles (h2) - sc-400137-LAC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR siRNA (h) - sc-35382 from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

FKHR shRNA Plasmid (h) - sc-35382-SH from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

FKHR shRNA (h) Lentiviral Particles - sc-35382-V from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

FoxO1/FKHR RNAi - H00002308-R01 from Novus Biologicals Category siRNA Prices $399.00 Sizes 20 nmol Species Reactivities Human

MISSION® esiRNA - EHU156591 from Sigma-Aldrich Category siRNA Prices $250.00 Sizes 50 µg

CompoZr® Knockout ZFN Kit - CKOZFN1356 from Sigma-Aldrich Category ZFN Kit Prices $4,999.00 Sizes 1 kt

CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1356 from Sigma-Aldrich Category ZFN Kit Prices $3,999.00 Sizes 1 kt

FOXO1 siRNA (Human) - CRH1615 from Cohesion Biosciences Category siRNA Prices $340.00, $510.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

FOXO1 - MBS8210551 from MyBioSource Prices $365.00, $515.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

FKHR CRISPR/Cas9 KO Plasmid (h2) - sc-400137-KO-2 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

FKHR HDR Plasmid (h2) - sc-400137-HDR-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

shRNA set against Human FOXO1 (NM_002015.3) - SHH296753 from Creative biogene Category siRNA Species Reactivities Human

shRNA set against Human FOXO1A(NM_002015.3) - SHH066989 from Creative biogene Category siRNA Species Reactivities Human

Human FOXO1 siRNA - orb262117 from Biorbyt Category siRNA Prices $485.00, $629.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

FOXO1 shRNA Plasmid (Human) - CHH1615 from Cohesion Biosciences Category siRNA Prices $500.00, $750.00 Sizes 150 µg, 300 µg Species Reactivities Human

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