DIXDC1

Gene Symbol DIXDC1
Entrez Gene 85458
Alt Symbol CCD1
Species Human
Gene Type protein-coding
Description DIX domain containing 1
Other Description coiled-coil protein DIX1|dixin
Swissprots Q96N69 E9PRV4 Q8IVY4 Q155Q3 Q9C0C8 A1A5D8 Q6PIK4 Q86SR7 Q6P2J8
Accessions EAW67171 EAW67172 EAW67173 Q155Q3 AB051522 BAB21826 AB074187 AF070621 AK055469 BAG51522 AK055899 BAB71039 AK074277 AK289807 BAF82496 AK294954 BAG58029 AU253693 BC033034 AAH33034 BC035509 AAH35509 BC041626 AAH41626 BC048294 AAH48294 BC064479 AAH64479 BC128600 AAI28601 BX370159 DA096120 DB358954 DB459100 DQ642016 ABG25914 HY037299 XM_005277726 XP_005277783 XM_005277727 XP_005277784 XM_005277728 XP_005277785 XM_011543045 XP_011541347 XM_011543046 XP_011541348 NM_001037954 NP_001033043 NM_001278542 NP_001265471 NM_033425 NP_219493
Function Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2. {ECO:0000269|PubMed:15262978, ECO:0000269|PubMed:21189423}.
Subcellular Location Isoform 2: Cytoplasm.
Tissue Specificity Ubiquitously expressed with higher expression in cardiac and skeletal muscles. {ECO:0000269|PubMed:16814745}.

DIXDC1 (D7P6Q) Rabbit mAb - 14315 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7P6Q
Western blot analysis of extracts from 293T cells mock transfected (-) or transfected with human DIXDC1 (hDIXDC1, +), using DIXDC1 (D7P6Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

DIXDC1 (A-6) - sc-377160 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DIXDC1 (A-6): sc-377160. Western blot analysis of DIXDC1 expression in mouse skeletal muscle (A) and rat skeletal muscle (B) tissue extracts.

DIXDC1 (C-14) - sc-109529 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

DIXDC1 (H-300) - sc-292126 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DIXDC1 (H-300): sc-292126. Western blot analysis of DIXDC1 expression in mouse brain tissue extract.

DIXDC1 (N-12) - sc-109530 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-DIXDC1 antibody - ab177517 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of DIXDC1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab177517 at 6 µg/mg lysate for IP. An alternative anti-DIXDC1 antibody was used at 1 µg/ml for subsequent western blot detection.Detection: Chemiluminescence with exposure time of 3 minutes.

Anti-DIXDC1 antibody - ab67763 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DIXDC1 antibody (ab67763) at 1/500 dilutionLane 1 : DIXDC1 transfected lysateLane 2 : Non transfected lysate. Lysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Anti-DIXDC1 antibody - ab99283 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-DIXDC1 antibody (ab99283) at 0.5 µg/ml + Human skeletal muscle lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-DIXDC1 antibody [6B6] - ab192776 from Abcam

Prices $407.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 6B6
Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney tissue labeling DIXDC1 with ab192776 at 15 µg/ml.

Anti-DIXDC1 Antibody (clone 1B3) - LS-C174428 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B3
Immunofluorescent staining of HeLa cells using anti-DIXDC1 mouse monoclonal antibody.

Anti-DIXDC1 Antibody (clone 6B6) IHC-plus™ - LS-B10934 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 6B6
Anti-DIXDC1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10934 concentration 15 ug/ml.

Anti-DIXDC1 Antibody (aa100-150) - LS-C290760 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DIXDC1 Antibody (aa225-275) - LS-C290761 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DIXDC1 Antibody (aa550-600) - LS-C290762 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DIXDC1 Antibody (aa450-461) - LS-C108943 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
DIXDC1 antibody (0.5 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DIXDC1 Antibody - LS-C307875 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DIXDC1 Antibody (clone 6D4) - LS-C174174 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6D4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DIXDC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DIXDC1.

Anti-DIXDC1 Antibody (aa633-683) - LS-C290763 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human DIXDC1 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-DIXDC1 Antibody (Internal) - LS-C345330 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of DIXDC1 Antibody with human 786-0 Whole Cell lysate.

Anti-DIXDC1 Antibody (clone 1A2) - LS-C337911 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DIXDC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DIXDC1.

Ccd1/DIXDC1 Antibody - NBP1-46147 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: Ccd1/DIXDC1 Antibody [NBP1-46147] - NBP1-46147 (0.5ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Ccd1/DIXDC1 Antibody - H00085458-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: Ccd1/DIXDC1 Antibody [H00085458-B01P] - Analysis of DIXDC1 expression in transfected 293T cell line by DIXDC1 polyclonal antibody. Lane 1: DIXDC1 transfected lysate(51.92 KDa). Lane 2: Non-transfected lysate.

Anti-DIXDC1 (446-460) antibody produced in rabbit - SAB1103026 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human FLAG<SUP>®</SUP>-DIXDC1 fusion protein was separated on SDS-PAGE, blotted with Anti-DIXDC1 (446-460) (<B>Cat. No. SAB1103026</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + DIXDC1 immunizing peptide (human, 446-460)<BR/>3. Negative control (only secondary antibody)<BR/>4. Anti-FLAG<SUP>®</SUP> antibody (<B>Cat. No. F7425</B>), working concentration 2 μg/ml.

Anti-DIXDC1 antibody produced in goat - SAB2501561 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-DIXDC1 <B>Cat. No. SAB2501561</B>: (0.5 μg/mL) staining of Human Skeletal Muscle lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DIXDC1 antibody produced in mouse - SAB1408150 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-DIXDC1: <B>Cat. No. SAB1408150</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~51.9 kDa using DIXDC1 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.
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