| Gene Symbol | Chst4 |
|---|---|
| Entrez Gene | 26887 |
| Alt Symbol | GST-3, Gn6st-2, HEC-GlcNAc6ST |
| Species | Mouse |
| Gene Type | protein-coding |
| Description | carbohydrate (chondroitin 6/keratan) sulfotransferase 4 |
| Other Description | L-selectin ligand sulfotransferase|LSST|N-acetylglucosamine 6-O-sulfotransferase 2|carbohydrate sulfotransferase 4|galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3|glcNAc6ST-2|high endothelial cell GlcNAC-6-sulphotransferase|high endothelial cells N-acetylglucosamine 6-O-sulfotransferase |
| Swissprots | Q9R1I1 Q9WUE5 |
| Accessions | AAD33016 EDL11448 EDL11449 Q9R1I1 AA672072 AF109155 AAD45579 AI614882 AK009113 BAB26078 BC057886 AAH57886 XM_006531043 XP_006531106 XM_006531044 XP_006531107 XM_006531045 XP_006531108 NM_011998 NP_036128 |
| Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of SELL ligand sialyl 6-sulfo Lewis X on SELL counter-receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular loc |
| Subcellular Location | Golgi apparatus membrane {ECO:0000269|PubMed:15111310}; Single-pass type II membrane protein {ECO:0000269|PubMed:15111310}. |
| Tissue Specificity | Specifically expressed in HEV. {ECO:0000269|PubMed:10435581}. |
| Top Pathways | Glycosaminoglycan biosynthesis - keratan sulfate |
HEC-GlcNAc6ST CRISPR/Cas9 KO Plasmid (m) - sc-424117 from Santa Cruz Biotechnology
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HEC-GlcNAc6ST HDR Plasmid (m) - sc-424117-HDR from Santa Cruz Biotechnology
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HEC-GlcNAc6ST Double Nickase Plasmid (m) - sc-424117-NIC from Santa Cruz Biotechnology
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HEC-GlcNAc6ST Double Nickase Plasmid (m2) - sc-424117-NIC-2 from Santa Cruz Biotechnology
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HEC-GlcNAc6ST CRISPR Activation Plasmid (m) - sc-424117-ACT from Santa Cruz Biotechnology
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HEC-GlcNAc6ST CRISPR Activation Plasmid (m2) - sc-424117-ACT-2 from Santa Cruz Biotechnology
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HEC-GlcNAc6ST Lentiviral Activation Particles (m) - sc-424117-LAC from Santa Cruz Biotechnology
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HEC-GlcNAc6ST Lentiviral Activation Particles (m2) - sc-424117-LAC-2 from Santa Cruz Biotechnology
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HEC-GlcNAc6ST siRNA (m) - sc-60777 from Santa Cruz Biotechnology
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HEC-GlcNAc6ST shRNA Plasmid (m) - sc-60777-SH from Santa Cruz Biotechnology
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HEC-GlcNAc6ST shRNA (m) Lentiviral Particles - sc-60777-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU011601 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN28749 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND28749 from Sigma-Aldrich
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CHST4 siRNA (Mouse) - CRM4790 from Cohesion Biosciences
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CHST4 - MBS8239605 from MyBioSource
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shRNA set against Mouse CHST4 (NM_011998.4) - SHH263817 from Creative biogene
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shRNA set against Mouse Chst4(NM_011998.4) - SHG178217 from Creative biogene
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Mouse CHST4 siRNA - orb284266 from Biorbyt
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CHST4 shRNA Plasmid (Mouse) - CHM4790 from Cohesion Biosciences
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CHST4 miRNA 3'UTR clone - MiUTR1M-03033 from Creative biogene
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