PAFAH1B1

Gene Symbol PAFAH1B1
Entrez Gene 5048
Alt Symbol LIS1, LIS2, MDCR, MDS, PAFAH
Species Human
Gene Type protein-coding
Description platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
Other Description lissencephaly 1 protein|platelet-activating factor acetylhydrolase IB subunit alpha|platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD)|platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
Swissprots Q8WZ89 B2R7Q7 Q8WZ88 P43034
Accessions AAC51111 EAW90536 P43034 AF208835 AF208836 AF208837 AAL34972 AF208838 AAL34973 AF400434 AAK92483 AK293918 BAG57299 AK303015 BAG64145 AK313078 BAG35904 BC064638 AAH64638 BX538346 CAD98141 L13385 AAA02880 L13386 AAA02881 L13387 AAA02882 L13388 L25107 S81396 XM_011523901 XP_011522203 XM_011523902 XP_011522204 XM_011523903 XP_011522205 XM_011523904 XP_011522206 NM_000430 NP_000421
Function Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration i
Subcellular Location Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle {ECO:0000255|HAMAP- Rule:MF_03141}. Nucleus membrane {ECO:0000255|HAMAP- Rule:MF_03141}. Note=Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane. {ECO:0000250}.
Tissue Specificity Fairly ubiquitous expression in both the frontal and occipital areas of the brain.
Top Pathways Ether lipid metabolism

LIS1 Antibody - 12453 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using LIS1 Antibody.

LIS1 (C-7) - sc-393320 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LIS1 (C-7): sc-393320. Western blot analysis of LIS1 expression in HEK293T (A), Caki-1 (B) and KNRK (C) whole cell lysates and mouse brain (D) and rat brain (E) tissue extracts.

LIS1 (G-3) - sc-17817 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LIS1 (G-3): sc-17817. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in ductus seminiferus and cytoplasmic staining in Leydig cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

LIS1 (H-7) - sc-374586 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LIS1 (H-7): sc-374586. Western blot analysis of LIS1 expression in non-transfected 293T: sc-117752 (A), mouse LIS1 transfected 293T: sc-125547 (B) and Caki-1 (C) whole cell lysates.

LIS1 (H-300) - sc-15319 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LIS1 (H-300): sc-15319. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts and Leydig cells.

LIS1 (K-16) - sc-14936 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LIS1 (N-19) - sc-7577 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LIS1 (N-19): sc-7577. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain showing cytoplasmic staining (A). Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization (B).

Anti-LIS1 antibody - ab68598 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LIS1 antibody (ab68598) at 1 µg/mlLane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : Rat Thymus Tissue Lysate Lane 4 : Liver (Mouse) Tissue Lysate Lane 5 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-LIS1 antibody - ab2656 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-LIS1 antibody (ab2656) at 0.1 µg/ml + Rat ovary lysate in RIPA buffer. at 35 µg

Anti-LIS1 antibody - ab115357 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab115357, at 5 µg/ml, staining LIS1 in formalin-fixed, paraffin-embedded Human Thymus tissue by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-LIS1 antibody [EPR3335(2)] - ab109630 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3335(2)
Overlay histogram showing SHSY-5Y cells stained with ab109630 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109630, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PAFAH1B1 / LIS1 Antibody (aa1-17) - LS-C3079 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAFAH1B1 / LIS1 Antibody (aa294-310) - LS-C3080 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAFAH1B1 / LIS1 Antibody - LS-C71732 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAFAH1B1 / LIS1 Antibody - LS-C125887 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PAFAH1B1 / LIS1 Antibody (aa1-50) - LS-C287304 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human, Mouse and Rat LIS-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, K562, mouse TCMK-1, and rat C6 cells. Antibodies: Affinity purified rabbit anti-LIS-1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-PAFAH1B1 / LIS1 Antibody (aa71-120) - LS-C111803 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PAFAH1B1 / LIS1 antibody LS-C111803 Western blot of Fetal Lung lysate.

Anti-PAFAH1B1 / LIS1 Antibody (clone 2C12) - LS-C197836 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C12
Detection limit for recombinant GST tagged PAFAH1B1 is approximately 0.03 ng/ml as a capture antibody.

Anti-PAFAH1B1 / LIS1 Antibody (aa91-125) - LS-C344547 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysates from HeLa, K562, SH-SY5Y, rat C6 cell line (from left to right) with PAFAH1B1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Anti-PAFAH1B1 / LIS1 Antibody (aa76-110) - LS-C344456 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysates from HeLa, mouse C2C12, rat C6 cell line (from left to right) with PAFAH1B1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Anti-PAFAH1B1 / LIS1 Antibody (Internal, clone EPR3335(2)) - LS-C138061 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3335(2)
Western blot analysis on (A) fetal brain, (B) HeLa, (C) SH-SY5Y, and (D) 293T cell lysates using anti-LIS1 antibody.

Anti-PAFAH1B1 / LIS1 Antibody (N-Terminus) IHC-plus™ - LS-B8228 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-LIS1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8228 dilution 5-7.5 ug/ml.

Anti-PAFAH1B1 / LIS1 Antibody (C-Terminus) IHC-plus™ - LS-B1383 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PAFAH1B1 / LIS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1383 concentration 5 ug/ml.

Anti-PAFAH1B1 / LIS1 Antibody (aa397-410) IHC-plus™ - LS-B3029 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-PAFAH1B1 / LIS1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3029 concentration 75 ug/ml.

LIS1 Antibody - NBP1-87769 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LIS1 Antibody [NBP1-87769] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Search more