NUP85

Gene Symbol NUP85
Entrez Gene 79902
Alt Symbol FROUNT, Nup75
Species Human
Gene Type protein-coding
Description nucleoporin 85kDa
Other Description nuclear pore complex protein Nup85|nucleoporin Nup75|nucleoporin Nup85
Swissprots Q9H9U1 B4DPW1 B4DMQ3 Q8NDI4 Q9BW27
Accessions EAW89251 Q9BW27 AF498261 AAM18528 AF514995 AAM76706 AK022611 BAB14130 AK124501 BAG54043 AK295694 BAH12158 AK297574 BAG59965 AK298519 BAG60723 AK302426 BAG63729 AL833893 CAD38749 BC000697 AAH00697 BM973048 DA375719 DA787201 DC424627 XM_005257690 XP_005257747 XM_005257692 XP_005257749 XM_005257693 XP_005257750 XM_006722094 XP_006722157 XM_011525267 XP_011523569 XM_011525268 XP_011523570 XR_243683 XR_429921 XR_934552 NM_001303276 NP_001290205 NM_024844 NP_079120
Function Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. {ECO:0000269|PubMed:12718872, ECO:0000269|PubMed:15995708, ECO:0000269|PubMed:16807356}.
Subcellular Location Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Nucleus membrane. Note=During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells.
Top Pathways RNA transport

Pericentrin 1 (D-4) - sc-376111 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pericentrin 1 (D-4): sc-376111. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.

Pericentrin 1 (H-300) - sc-68927 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Pericentrin 1 (H-300): sc-68927. Western blot analysis of Pericentrin 1 expression in HeLa whole cell lysate.

Pericentrin 1 (E-17) - sc-28142 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pericentrin 1 (N-17) - sc-28140 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Pericentrin 1 antibody - ab176801 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Pericentrin 1 antibody (ab176801) at 0.1 µg/mlLane 1 : 293T whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : TCMK-1 whole cell lysateLane 5 : NIH 3T3 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-Pericentrin 1 antibody - ab19044 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab19044 stained T47D cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA  / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19044 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Pericentrin 1 antibody - ab168839 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Pericentrin 1 antibody (ab168839) at 0.1 µg/mlLane 1 : 293T whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : Jurkat whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-Pericentrin 1 antibody - ab169077 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Pericentrin 1 antibody (ab169077) at 1 µg/mlLane 1 : Pericentrin 1-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-NUP85 / Pericentrin 1 Antibody - LS-C131355 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-NUP85 / Pericentrin 1 Antibody (aa1-50) - LS-C290625 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse NUP85 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUP85 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-NUP85 / Pericentrin 1 Antibody - LS-C335359 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using NUP85 antibody.

Anti-NUP85 / Pericentrin 1 Antibody (aa575-625) - LS-C290621 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human NUP85 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-NUP85 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-NUP85 / Pericentrin 1 Antibody (aa598-610) - LS-C20189 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
(1 ug/ml) Staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Nucleoporin NUP85/Pericentrin 1 Antibody - AF5976 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Nucleoporin NUP85 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5976) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Nucleoporin NUP85/Pericentrin 1 Antibody (783403) [Unconjugated] - MAB5976 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 783403
Western blot shows lysates of NR8383 rat alveolar macrophage cell line, K562 human chronic myelogenous leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, M1 mouse myeloid leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Nucleoporin NUP85 Monoclonal Antibody (Catalog # MAB5976) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Nucleoporin NUP85/Pericentrin 1 Antibody - NBP2-31860 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: Nucleoporin NUP85/Pericentrin 1 Antibody [NBP2-31860] - Pericentrin 1 Antibody [NBP2-31860] - Staining of human oral mucosa shows moderate nucleolar positivity in squamous epithelial cells.

Nucleoporin NUP85/Pericentrin 1 Antibody - NB100-1355 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: Nucleoporin NUP85/Pericentrin 1 Antibody [NB100-1355] - NB100-1355 (1 ug/ml) staining of Hela lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Nucleoporin NUP85/Pericentrin 1 Antibody - H00079902-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: Nucleoporin NUP85/Pericentrin 1 Antibody [H00079902-B01] - analysis of NUP85 expression in transfected 293T cell line by NUP85 MaxPab polyclonal antibody (B01). Lane 1: NUP85 transfected lysate (75.00 KDa). Lane 2: Non-transfected lysate.

Nucleoporin NUP85/Pericentrin 1 Antibody - H00079902-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: Nucleoporin NUP85/Pericentrin 1 Antibody [H00079902-B01P] - Analysis of NUP85 expression in transfected 293T cell line by NUP85 polyclonal antibody. Lane 1: NUP85 transfected lysate(72.16 KDa). Lane 2: Non-transfected lysate.

Pericentrin Antibody - NB100-63208 from Novus Biologicals

Prices $299.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Nucleoporin NUP85/Pericentrin 1 Antibody (3E9) - H00079902-M04 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9

Anti-NUP85 antibody produced in mouse - SAB1407993 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-NUP85: <B>Cat. No. SAB1407993</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~72.2 kDa using NUP85 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-NUP85 antibody produced in rabbit - HPA062595 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NUP85: <B>Cat. No. HPA062595</B>: Immunohistochemical staining of Human oral mucosa shows moderate nucleolar positivity in squamous epithelial cells.

Monoclonal Anti-NUP85 antibody produced in mouse - SAB1401833 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E9

Anti-Pericentrin 1 /NUP85 antibody produced in goat - SAB2500774 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-Pericentrin 1 / NUP85: <B>Cat. No. SAB2500774</B>: (1 μg/mL) staining of HeLa lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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