SYNE2
| Gene Symbol | SYNE2 |
|---|---|
| Entrez Gene | 23224 |
| Alt Symbol | EDMD5, NUA, NUANCE, Nesp2, Nesprin-2, SYNE-2, TROPH |
| Species | Human |
| Gene Type | protein-coding |
| Description | spectrin repeat containing, nuclear envelope 2 |
| Other Description | nesprin 2|nesprin-2|nuclear envelope spectrin repeat protein 2|nucleus and actin connecting element protein|polytrophin|synaptic nuclear envelope protein 2|synaptic nuclei expressed gene 2 |
| Swissprots | Q8WXH1 Q8NF49 Q9Y2L4 Q8TER7 Q9UFQ4 B4DPR6 Q8WWW3 I6XXQ5 Q540G1 Q86YP9 Q8WWW5 Q8N1S3 Q8WWW4 B4DND7 Q9Y4R1 Q8WXH0 |
| Accessions | EAW80838 EAW80839 EAW80840 EAW80841 EAW80842 EAW80843 EAW80844 Q8WXH0 AB023228 BAA76855 AF357236 AAQ15171 AF435010 AAL33547 AF435011 AAL33548 AF495911 AAN60443 AI685725 AK001876 AK074055 BAB84881 AK090430 BAC03411 AK095087 AK095241 BAC04506 AK125715 AK127612 AK128631 AK297874 BAG60199 AK298465 BAG60678 AL080133 CAB45729 AL117404 CAB55905 AY061757 AAL33800 AY061758 AAL33801 AY061759 AAL33802 AY184204 AAO27772 AY184205 AAO27773 AY927627 BC036941 BC042134 AAH42134 BC071873 AAH71873 BC154417 BX537642 BX538095 CAD98013 BX640974 CAE45988 BX648234 CR749324 CAH18179 DA825926 DB271849 JQ754367 AFN44385 XM_005267454 XP_005267511 XM_005267456 XP_005267513 XM_005267457 XP_005267514 XM_005267458 XP_005267515 XM_005267459 XP_005267516 XM_006720084 XP_006720147 XM_011536574 XP_011534876 XM_011536575 XP_011534877 XM_011536576 XP_011534878 XM_011536577 XP_011534879 XM_011536578 XP_011534880 XM_011536579 XP_011534881 XM_011536580 XP_011534882 XM_011536581 XP_011534883 XM_011536582 XP_011534884 XM_011536583 XP_011534885 XM_011536584 XP_011534886 NM_015180 NP_055995 NM_182910 NP_878914 NM_182913 NP_878917 NM_182914 NP_878918 NM_182912 |
| Function | Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein- containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for centrosome migration to the apical cell surface during early ciliogenesis. {ECO:0000269|PubMed:12118075, ECO:0000269|PubMed:18396275, ECO:0000269|PubMed:19596800, ECO:0000269|PubMed:20724637 |
| Subcellular Location | Nucleus outer membrane {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Sarcoplasmic reticulum membrane {ECO:0000305|PubMed:15671068}; Single-pass type IV membrane protein {ECO:0000305}. Cell membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. Cytoplasm, cytoskeleton. Mitochondrion. Nucleus, nucleoplasm. Cytoplasm, myofibril, sarcomere, Z line {ECO:0000305|PubMed:15671068}. Note=Different isoform patterns are found in the different compartments of the cell. The isoforms having the C-terminal transmembrane span can be found in several organellar membranes like the nuclear envelope, the sarcoplasmic reticulum of myoblasts, or the lamellipodia and focal adhesions at the cell membrane. The largest part of the outer nuclear membrane-associated protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during |
| Tissue Specificity | Widely expressed, with higher level in kidney, adult and fetal liver, stomach and placenta. Weakly expressed in skeletal muscle and brain. Isoform 5 is highly expressed in pancreas, skeletal muscle and heart. {ECO:0000269|PubMed:15671068}. |
Syne-2 CRISPR/Cas9 KO Plasmid (h) - sc-403318 from Santa Cruz Biotechnology
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Syne-2 HDR Plasmid (h) - sc-403318-HDR from Santa Cruz Biotechnology
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Syne-2 Double Nickase Plasmid (h) - sc-403318-NIC from Santa Cruz Biotechnology
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Syne-2 Double Nickase Plasmid (h2) - sc-403318-NIC-2 from Santa Cruz Biotechnology
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Syne-2 CRISPR Activation Plasmid (h) - sc-403318-ACT from Santa Cruz Biotechnology
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Syne-2 CRISPR Activation Plasmid (h2) - sc-403318-ACT-2 from Santa Cruz Biotechnology
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Syne-2 Lentiviral Activation Particles (h) - sc-403318-LAC from Santa Cruz Biotechnology
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Syne-2 Lentiviral Activation Particles (h2) - sc-403318-LAC-2 from Santa Cruz Biotechnology
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Syne-2 siRNA (h) - sc-61630 from Santa Cruz Biotechnology
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Syne-2 shRNA Plasmid (h) - sc-61630-SH from Santa Cruz Biotechnology
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Syne-2 shRNA (h) Lentiviral Particles - sc-61630-V from Santa Cruz Biotechnology
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Nesprin 2 RNAi - H00023224-R02 from Novus Biologicals
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Nesprin 2 RNAi - H00023224-R01 from Novus Biologicals
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Nesprin 2 RNAi - H00023224-R03 from Novus Biologicals
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MISSION® esiRNA - EHU099001 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20892 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20892 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR07138 from Sigma-Aldrich
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SYNE2 siRNA (Human) - CRH8101 from Cohesion Biosciences
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Nesprin 2 RNAi - H00023224-R04 from Novus Biologicals
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SYNE2 - MBS8229893 from MyBioSource
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shRNA set against Human SYNE2(NM_182910.2) - SHL050592 from Creative biogene
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shRNA set against Human SYNE2(NM_182914.2) - SHL050610 from Creative biogene
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shRNA set against Human SYNE2(NM_015180.4) - SHL050646 from Creative biogene
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shRNA set against Human SYNE2(NM_182913.2) - SHL050664 from Creative biogene
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