VRK2

Gene Symbol VRK2
Entrez Gene 7444
Alt Symbol -
Species Human
Gene Type protein-coding
Description vaccinia related kinase 2
Other Description serine/threonine-protein kinase VRK2|vaccinia virus B1R-related kinase 2
Swissprots Q86Y07 Q8IXI5 D6W5D4 Q86Y12 D6W5D6 Q86Y11 Q86Y09 Q53S39 Q53EU9 Q53S77 Q86Y08 Q53TU1 Q86Y10 B4DKL0 Q49AK9 Q99987
Accessions AAX93262 AAY14648 AAY15019 EAX00062 EAX00063 EAX00064 EAX00065 EAX00066 EAX00067 EAX00068 Q86Y07 AB000450 BAA19109 AJ512204 CAD54446 AK058199 AK223540 BAD97260 AK292727 BAF85416 AK295193 BAH12007 AK296611 BAG59222 AK307113 AK311205 AY228367 AAO73047 AY228368 AAO73048 AY228369 AAO73049 AY228370 AAO73050 AY228371 AAO73051 AY228372 AAO73052 BC021663 AAH21663 BC027854 AAH27854 BC036434 BI906749 DA273734 DB227029 DQ890932 ABM81858 DQ894094 ABM85020 XM_005264540 XP_005264597 XM_005264541 XP_005264598 XM_006712090 XP_006712153 XM_006712091 XP_006712154 XM_006712092 XP_006712155 XM_006712093 XP_006712156 XM_011533092 XP_011531394 XM_011533093 XP_011531395 XM_011533094 XP_011531396 NM_001130480 NP_001123952 NM_001130481 NP_001123953 NM_001130482 NP_001123954 NM_001130483 NP_001123955 NM_001288836 NP_001275765 NM_001288837 NP_001275766 NM_001288838 NP_001275767 NM_001288839 NP_001275768 NM_006296 NP_006287
Function Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevan
Subcellular Location Isoform 1: Cytoplasm. Endoplasmic reticulum membrane {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}. Mitochondrion membrane {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}.
Tissue Specificity Isoform 1 and isoform 2 are expressed in various tumor cell lines. Expression of isoform 1 inversely correlates with ERBB2 in breast carcinomas (at protein level). Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis. {ECO:0000269|PubMed:16704422, ECO:0000269|PubMed:20679487, ECO:0000269|PubMed:9344656}.

VRK2 (H-5) - sc-365199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VRK2 (H-5): sc-365199. Western blot analysis of VRK2 expression in K-562 whole cell lysate.

VRK2 (H-255) - sc-98733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VRK2 (H-255): sc-98733. Western blot analysis of VRK2 expression in K-562 whole cell lysate.

VRK2 (K-13) - sc-109149 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

VRK2 (M-41) - sc-98732 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VRK2 (M-41): sc-98732. Western blot analysis of VRK2 expression in A2058 whole cell lysate.

VRK2 (Y-13) - sc-109151 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-VRK2 antibody - ab58052 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing K562 cells stained with ab58052 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58052, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-VRK2 antibody - ab92626 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VRK2 antibody (ab92626) at 1/500 dilutionLane 1 : Jurkat cell lysate in absence of blocking peptideLane 2 : Jurkat cell lysate in presence of blocking peptide (10 µg)Lysates/proteins at 30 µg per lane.

Anti-VRK2 antibody - ab184875 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of U-2 OS cells labeling VRK2 with ab184875 at 4 µg/ml.

Anti-VRK2 antibody - ab190309 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-VRK2 antibody (ab190309) at 2 µg/ml + K562 nuclear lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-VRK2 Antibody (clone 3B10) - LS-C198433 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B10
Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10 ug/ml]

Anti-VRK2 Antibody (aa1-508) - LS-C132838 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Anti-VRK2 Antibody (aa354-381, AP) - LS-C273385 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK2 Antibody (aa354-381, APC) - LS-C273386 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK2 Antibody (aa354-381, FITC) - LS-C273388 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK2 Antibody (aa354-381, Biotin) - LS-C273387 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK2 Antibody (aa354-381, Azide-free, HRP) - LS-C273389 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK2 Antibody (aa354-381, PE) - LS-C273390 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK2 Antibody (aa353-381) - LS-C326297 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK2 Antibody (aa260-360, Azide-free) - LS-C330729 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-VRK2 Antibody (aa171-220) - LS-C120375 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Jurkat cells, using VRK2 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-VRK2 Antibody (aa354-381) - LS-C158285 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Mouse Vrk2 Antibody western blot of mouse liver tissue lysates (35 ug/lane). The Vrk2 antibody detected the Vrk2 protein (arrow).

VRK2 Antibody (3B10) - H00007444-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B10

VRK2 Antibody - H00007444-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

VRK2 Antibody - NBP1-68671 from Novus Biologicals

Prices $229.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: VRK2 Antibody [NBP1-68671] - Analysis of extracts from Jurkat cells, using VRK2 Antibody. The lane on the right is treated with the synthesized peptide.

VRK2 Antibody - H00007444-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Search more