NPHS1

Gene Symbol NPHS1
Entrez Gene 4868
Alt Symbol CNF, NPHN, nephrin
Species Human
Gene Type protein-coding
Description nephrosis 1, congenital, Finnish type (nephrin)
Other Description nephrin|renal glomerulus-specific cell adhesion receptor
Swissprots C3RX61 O60500 A6NDH2
Accessions AAB80768 O60500 AF035835 AAC39687 AF126957 AAF36451 AF190637 AAG17141 AK310731 BC156195 AAI56196 BC156935 AAI56936 BX103667 EU642886 ACH99862 EU642887 NM_004646 NP_004637
Function Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). {ECO:0000250}.
Subcellular Location Cell membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. {ECO:0000269|PubMed:10393930, ECO:0000269|PubMed:10550324}.
Tissue Specificity Specifically expressed in podocytes of kidney glomeruli.

nephrin (B-12) - sc-377246 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
nephrin (B-12): sc-377246. Western blot analysis of nephrin expression in human kidney tissue extract.

nephrin (E-1) - sc-376568 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
nephrin (E-1): sc-376568. Western blot analysis of nephrin expression in human kidney tissue extract.

nephrin (G-20) - sc-32530 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
nephrin (G-20): sc-32530. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing membrane staining of cells in glomeruli and cytoplasmic staining of cells in tubules.

nephrin (G-8) - sc-376522 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
nephrin (G-8): sc-376522. Western blot analysis of nephrin expression in human kidney tissue extract.

nephrin (H-300) - sc-28192 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
nephrin (H-300): sc-28192. Western blot analysis of nephrin expression in human kidney tissue extract.

nephrin (C-17) - sc-32529 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

nephrin (N-20) - sc-19000 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
nephrin (N-20): sc-19000. Western blot analysis of nephrin expression in NRK (A) and KNRK (B) whole cell lysates.

nephrin (N-16) - sc-32532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
nephrin (N-16): sc-32532. Western blot analysis of nephrin expression in KNRK whole cell lysate.

Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] - ab80299 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPTPG1
All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/50000 dilutionLane 1 : HEK293T cells transfected with wild type CD 16/7 NephrinLane 2 : HEK293T cells transfected with active tyrosine kinase (vSrc)Lane 3 : HEK293T cells transfected with Nephrin mutated at Tyr1176 and vSrcLane 4 : HEK293T cells transfected with Nephrin mutated at Tyr 1193 and vSrc using Phospho Nephrin (pY1176/1193)SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Nephrin (phospho Y1217) antibody [EPTPG3] - ab80298 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPTPG3
All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/50000 dilutionLane 1 : HEK293T cells transfected with wild type CD 16/7 NephrinLane 2 : HEK293T cells transfected with active tyrosine kinase (vSrc)Lane 3 : HEK293T cells transfected with Nephrin mutated at Tyr1217 and vSrc using Phospho Nephrin (pY1176/1193)Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Nephrin antibody - ab58968 from Abcam

Prices $394.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nephrin antibody (ab58968)Lane 1 : HEK293 cell lysateLane 2 : Mouse kidney lysate

Anti-Nephrin antibody - ab72908 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Nephrin antibody (ab72908) at 1 µg/ml + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 10 µgSecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Nephrin antibody - ab85379 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of Nephrin staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with 85379, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Nephrin antibody - ab92887 from Abcam

Prices $376.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab92887, at 1/50 dilution staining Nephrin in formalin-fixed, paraffin-embedded Human kidney tissue.

Anti-Nephrin antibody [G17-H] - ab136927 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone G17-H
All lanes : Anti-Nephrin antibody [G17-H] (ab136927) at 1/2000 dilutionLane 1 : Recombinant Mouse Nephrin (extracellular domain, Gln 37–Thr 1049) at 0.05 µgLane 2 : Recombinant Mouse Nephrin (extracellular domain, Gln 37–Thr 1049) at 0.1 µgLane 3 : Recombinant Mouse Nephrin (extracellular domain, Gln 37–Thr 1049) at 0.2 µg

Anti-Nephrin antibody, prediluted - ab82113 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab82113, prediluted, staining Nephrin in formalin fixed, paraffin embedded human kidney by Immunohistochemistry.

Anti-Nephrin antibody [Y17-R] - ab136894 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone Y17-R
Overlay histogram showing HEK293 cells stained with ab136894 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab136894, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed HEK293 cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Nephrin antibody - C-terminal - ab183099 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nephrin antibody - C-terminal (ab183099) at 1/800 dilutionLane 1 : Mouse spleen tissue lysateLane 2 : Mouse kidney tissue lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution

Anti-NPHS1 / Nephrin Antibody (aa23-383) - LS-C343979 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Nephrin antibody IHC-paraffin: Human Intestinal Cancer Tissue.

Anti-NPHS1 / Nephrin Antibody - LS-C332338 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using NPHS1 antibody.

Anti-NPHS1 / Nephrin Antibody (C-Terminus) IHC-plus™ - LS-B1382 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Nephrin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1382 concentration 5 ug/ml.

Anti-NPHS1 / Nephrin Antibody (aa23-1029) - LS-C37499 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-NPHS1 / Nephrin Antibody - LS-C83675 from LifeSpan Bioscience

Prices $275.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-NPHS1 / Nephrin Antibody (C-Terminus) - LS-C83682 from LifeSpan Bioscience

Prices $345.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-NPHS1 / Nephrin Antibody (aa1243-1256) - LS-C86180 from LifeSpan Bioscience

Prices $695.00
Sizes 100 µl
Host Guinea Pig
Clonality Polyclonal
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