POU4F3

Gene Symbol POU4F3
Entrez Gene 5459
Alt Symbol BRN3C, DFNA15
Species Human
Gene Type protein-coding
Description POU class 4 homeobox 3
Other Description POU domain, class 4, transcription factor 3|brain-3C|brain-specific homeobox/POU domain protein 3C|brn-3C
Swissprots Q2M3F8 O60557 Q15319
Accessions AAA57160 AAC06203 EAW61843 EAW61844 EAW61845 EAW61846 Q15319 BC104923 AAI04924 BC112207 AAI12208 EU446649 ABZ92178 HQ258176 ADR82930 NM_002700 NP_002691
Function May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Subcellular Location Nucleus.
Tissue Specificity Brain. Seems to be specific to the retina. {ECO:0000269|PubMed:7623109, ECO:0000269|PubMed:9506947}.

Brn-3c (QQ8) - sc-81980 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Brn-3c (QQ8): sc-81980. Western blot analysis of Brn-3c expression in HeLa nuclear extract.

Anti-POU4F3 antibody - ab58128 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HepG2 cells stained with ab58128 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58128, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-POU4F3 antibody - ab106797 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-POU4F3 antibody (ab106797) at 0.2 µg/ml + Human Heart lysate at 35 µg

Anti-POU4F3 antibody - ab84170 from Abcam

Prices $359.00, $370.00
Sizes 100 µg, 400 µl
Host Rabbit
Clonality Polyclonal
Anti-POU4F3 antibody (ab84170) at 1/100 dilution + HL60 cell line lysates at 35 µg

Anti-POU4F3 / BRN3C Antibody (aa231-280) - LS-C291280 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-POU4F3 / BRN3C Antibody (aa263-308) - LS-C313686 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-POU4F3 / BRN3C Antibody (aa100-191, Azide-free) - LS-C328290 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-POU4F3 / BRN3C Antibody (N-Terminus) - LS-C145531 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
POU4F3 antibody LS-C145531 Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml.

Anti-POU4F3 / BRN3C Antibody (aa211-260) - LS-C30803 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
POU4F3 antibody LS-C30803 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-POU4F3 / BRN3C Antibody (aa266-295) IHC-plus™ - LS-B11574 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-POU4F3 / BRN3C Antibody (aa256-267) - LS-C139587 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
POU4F3 antibody (0.2 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-POU4F3 / BRN3C Antibody (clone 5B8) - LS-C197954 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5B8
Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]

POU4F3 Antibody - NBP1-88349 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: POU4F3 Antibody [NBP1-88349] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

POU4F3 Antibody (5B8) - H00005459-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5B8
Western Blot: POU4F3 Antibody (5B8) [H00005459-M01] - POU4F3 monoclonal antibody (M01), clone 5B8 Analysis of POU4F3 expression in Hela S3 NE.

POU4F3 Antibody - NBP1-52036 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

POU4F3 Antibody - H00005459-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: POU4F3 Antibody [H00005459-B01P] - Analysis of POU4F3 expression in transfected 293T cell line by POU4F3 polyclonal antibody.  Lane 1: POU4F3 transfected lysate(37.18 KDa). Lane 2: Non-transfected lysate.

POU4F3 Antibody - H00005459-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

POU4F3 Antibody - H00005459-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-POU4F3 antibody produced in mouse - WH0005459M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5B8
<B>Immunoblotting</B><BR/>Monoclonal Anti-POU4F3, <B>Cat. No. WH0005459M1</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~37 kDa using Hela NE lysate.

Anti-POU4F3 antibody produced in rabbit - HPA038215 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-POU4F3: <B>Cat. No. HPA038215</B>: Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.

Anti-POU4F3 antibody produced in rabbit - AV33065 from Sigma-Aldrich

Prices $267.50, $267.50
Sizes 100 µg, 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-POU4F3: <B>Cat. No. AV33065</B>: Immunohistochemistry of POU4F3 in Human Kidney tissue with POU4F3 antibody at 4-8 μg/mL.

Anti-POU4F3 antibody produced in goat - SAB2501603 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-POU4F3 <B>Cat. No. SAB2501603</B>: (0.2 μg/mL) staining of Human Heart lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-POU4F3 antibody produced in mouse - SAB1406275 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-POU4F3: <B>Cat. No. SAB1406275</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~37.2 kDa using POU4F3 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

POU4F3 antibody - middle region (ARP33065_T100) - ARP33065_T100 from Aviva Systems Biology

Prices $229.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

POU4F3-Antibody-monoclonal-M01 - AT3386a from Abgent, a WuXi AppTec company

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5B8
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.75 KDa) .
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