PSMB7

Gene Symbol	PSMB7
Entrez Gene	5695
Alt Symbol	Z
Species	Human
Gene Type	protein-coding
Description	proteasome (prosome, macropain) subunit, beta type, 7
Other Description	macropain chain Z\|multicatalytic endopeptidase complex chain Z\|proteasome catalytic subunit 2\|proteasome subunit Z\|proteasome subunit alpha\|proteasome subunit beta type-7
Swissprots	Q5TBG6 Q9BWA7 B4E0P1 Q96AG8 Q99436
Accessions	EAW87582 EAW87583 Q99436 AJ420455 AK056654 AK098757 AK223007 BAD96727 AK223356 BAD97076 AK303460 BAG64503 AK308756 AK314302 BAG36956 BC000509 AAH00509 BC008414 BC017116 AAH17116 BT007218 AAP35882 CR456721 CAG33002 D38048 BAA07238 GU727627 ADU87629 U44425 AAD45962 NM_002799 NP_002790
Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
Subcellular Location	Cytoplasm {ECO:0000255\|PROSITE- ProRule:PRU00809, ECO:0000269\|PubMed:18549262}. Nucleus {ECO:0000269\|PubMed:18549262}.
Tissue Specificity	Expressed at a low level in colonic mucosa. Up-regulated in colorectal cancer tissues. {ECO:0000269\|PubMed:18549262}.
Top Pathways	Proteasome

PSMB7 (E1L5H) Rabbit mAb - 13207 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	E1L5H

Western blot analysis of extracts from various cell lines using PSMB7 (E1L5H) Rabbit mAb.

PSMB7 Antibody - 12197 from Cell Signaling Technology

Prices	$239.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from various cell lines using PSMB7 Antibody.

20S Proteasome β7 (S-14) - sc-54735 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

20S Proteasome β7 (D-14) - sc-54677 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-Proteasome 20S beta 7 (phospho S214) antibody - ab90626 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Proteasome 20S beta 7 antibody - ab37616 from Abcam

Prices	$406.00
Sizes	50 µg
Host	Chicken
Clonality	Polyclonal

Anti-Proteasome 20S beta 7 antibody (ab37616) at 1/2000 dilution + E coli derived fusion proteinSecondaryGoat anti IgY HRP at 1/1000 dilutiondeveloped using the ECL technique

Anti-Proteasome 20S beta 7 antibody - ab167320 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-Proteasome 20S beta 7 antibody (ab167320) at 1 µg/mlLane 1 : Proteasome 20S beta 7-transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.

Anti-Proteasome 20S beta 7 antibody - ab167516 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-Proteasome 20S beta 7 antibody (ab167516) at 1 µg/mlLane 1 : Proteasome 20S beta 7 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-Mouse IgG HRP at 1/2500 dilution

Anti-PSMB7 Antibody (clone 4D12) - LS-C173810 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4D12

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMB7 monoclonal antibody.

Anti-PSMB7 Antibody (aa58-277, clone 1B2) - LS-C337672 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1B2

Anti-PSMB7 Antibody (aa58-277, clone 4E3) - LS-C337712 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4E3

Anti-PSMB7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMB7.

Anti-PSMB7 Antibody (aa58-277, clone 4G9) - LS-C337653 from LifeSpan Bioscience

Prices	$350.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4G9

Anti-PSMB7 Antibody (phospho-Ser249) - LS-C145099 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PSMB7 Antibody - LS-C211771 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Proteasome subunit beta type-7 antibody at 2 ug/ml + 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:15000 dilution. Predicted band size: 30.5 kDa. Observed band size: 30.5 kDa Additional bands at: 60 kDa. We are unsure as to the identity of this extra band.

Anti-PSMB7 Antibody (HRP) - LS-C211772 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PSMB7 Antibody (clone 4E10) - LS-C173791 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	4E10

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMB7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMB7.

Anti-PSMB7 Antibody (FITC) - LS-C211775 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PSMB7 Antibody (clone MCP205) - LS-C343786 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	MCP205

Anti-PSMB7 Antibody (Biotin) - LS-C211776 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PSMB7 Antibody (clone MCP444) - LS-C343804 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	MCP444

Anti-PSMB7 Antibody (Internal) - LS-C139639 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

PSMB7 antibody (0.3 ug/ml) staining of HEK293 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PSMB7 Antibody (clone MCP219) - LS-C343805 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	MCP219

Anti-PSMB7 Antibody (aa150-200) - LS-C290497 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human PSMB7 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMB7 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-PSMB7 Antibody (clone 1E1) - LS-C173783 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1E1

IHC of paraffin-embedded Human pancreas tissue using anti-PSMB7 mouse monoclonal antibody.

Anti-PSMB7 Antibody (aa58-277, clone 1D1) - LS-C337665 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	1D1

IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PSMB7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

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