TFB1M

Gene Symbol	TFB1M
Entrez Gene	51106
Alt Symbol	CGI-75, CGI75, mtTFB, mtTFB1
Species	Human
Gene Type	protein-coding
Description	transcription factor B1, mitochondrial
Other Description	S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1\|dimethyladenosine transferase 1, mitochondrial\|h-mtTFB1\|hTFB1M\|homolog of yeast mitochondrial transcription factor B\|mitochondrial 12S rRNA dimethylase 1\|mitochondrial dimethyladenosine transferase 1
Swissprots	Q9Y384 Q05DR0 Q8WVM0
Accessions	ADP92254 ADP92255 ADP92256 ADP92257 ADP92258 ADP92259 ADP92260 ADP92261 ADP92262 ADP92263 ADP92264 ADP92265 ADP92266 ADP92267 ADP92268 ADP92269 ADP92270 ADP92271 ADP92272 ADP92273 ADP92274 ADP92275 ADP92276 ADP92277 ADP92278 ADP92279 ADP92280 ADP92281 ADP92282 ADP92283 ADP92284 ADP92285 ADP92286 ADP92287 ADP92288 ADP92289 ADP92290 ADP92291 ADP92292 ADP92293 EAW47690 Q8WVM0 AA405744 AF151833 AAD34070 AK022993 AK289484 BAF82173 BC005183 AAH05183 BC017788 AAH17788 BC054007 BU849912 CA430614 DQ891970 ABM82896 DQ895159 ABM86085 HG499813 XM_005267006 XP_005267063 XM_011535869 XP_011534171 XM_011535870 XP_011534172 XM_011535871 XP_011534173 XM_011535872 XP_011534174 XM_011535873 XP_011534175 XR_942460 NM_016020 NP_057104
Function	S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. {ECO:0000269\|PubMed:11809803, ECO:0000269\|PubMed:12068295, ECO:0000269\|PubMed:12897151}.
Subcellular Location	Mitochondrion {ECO:0000269\|PubMed:11809803}.
Tissue Specificity	Ubiquitously expressed. {ECO:0000269\|PubMed:12068295}.

TFB1M (C-15) - sc-169582 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

TFB1M (D-13) - sc-169583 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

TFB1M (X-22) - sc-134077 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

TFB1M (X-22): sc-134077. Western blot analysis of TFB1M expression in 721B whole cell lysate.

Anti-TFB1M antibody - ab69871 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-TFB1M antibody (ab69871) at 5 µg/ml + 721_B_lymphoblasts cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-TFB1M antibody - ab77729 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-TFB1M antibody (ab77729) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-TFB1M antibody - ab67558 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-TFB1M antibody (ab67558) at 1 µg/mlLane 1 : TFB1M transfected HEK293T lysateLane 2 : Non-transfected HEK293T lysate

Anti-TFB1M antibody - ab106774 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-TFB1M antibody (ab106774) at 0.3 µg/ml + A431 cell lysate at 35 µgdeveloped using the ECL technique

Anti-TFB1M antibody - ab184151 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-TFB1M antibody (ab184151) at 1/1000 dilutionLane 1 : 293T lysateLane 2 : A431 lysateLane 3 : HeLa lysateLane 4 : HepG2 lysateLysates/proteins at 30 µg per lane.

Anti-TFB1M antibody - N-terminal - ab157252 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-TFB1M antibody - N-terminal (ab157252) at 1/1000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : MDA-MB231 cell lysate

Anti-TFB1M Antibody (aa151-200) - LS-C80098 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Lane A: Marker. Lane B: 721_B_Iymphoblasts cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.

Anti-TFB1M Antibody (aa36-85) - LS-C109926 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

TFB1M antibody LS-C109926 Western blot of Fetal liver lysate.

Anti-TFB1M Antibody (aa116-346, clone 3G8) - LS-C339010 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	3G8

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFB1M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFB1M.

Anti-TFB1M Antibody (aa116-346, clone 11H1) - LS-C339009 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	11H1

Anti-TFB1M Antibody (aa116-346, clone 6G4) - LS-C339008 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	6G4

Anti-TFB1M Antibody (aa116-346, clone 10H8) - LS-C339104 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	10H8

Anti-TFB1M Antibody (aa325-337) - LS-C108135 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

TFB1M antibody (0.3 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TFB1M Antibody (aa116-346, clone 13H2) - LS-C339012 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	13H2

TFB1M Antibody - NBP2-13429 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: TFB1M Antibody [NBP2-13429] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

TFB1M Antibody - NBP1-51978 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Western Blot: TFB1M Antibody [NBP1-51978] - (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TFB1M Antibody - H00051106-D01P from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: TFB1M Antibody [H00051106-D01P] - Analysis of TFB1M expression in transfected 293T cell line by TFB1M polyclonal antibody.Lane 1: TFB1M transfected lysate(39.50 KDa).Lane 2: Non-transfected lysate.

TFB1M Antibody - H00051106-B02P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: TFB1M Antibody [H00051106-B02P] - Analysis of TFB1M expression in transfected 293T cell line by TFB1M polyclonal antibody. Lane 1: TFB1M transfected lysate(38.06 KDa). Lane 2: Non-transfected lysate.

TFB1M Antibody (4E4) - H00051106-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	4E4

Western Blot: TFB1M Antibody (4E4) [H00051106-M01] - TFB1M monoclonal antibody (M01), clone 4E4. Analysis of TFB1M expression in HeLa.

TFB1M Antibody - H00051106-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: TFB1M Antibody [H00051106-B01] - analysis of TFB1M expression in transfected 293T cell line by TFB1M MaxPab polyclonal antibody. Lane 1: TFB1M transfected lysate (38.17 KDa). Lane 2: Non-transfected lysate.

Anti-TFB1M antibody produced in mouse - SAB1407443 from Sigma-Aldrich

Prices	$347.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-TFB1M: <B>Cat. No. SAB1407443</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~38.1 kDa using TFB1M transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-TFB1M antibody produced in rabbit - AV34544 from Sigma-Aldrich

Prices	$267.50
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: 721_B_lymphoblasts (<B>Cat. No. AV34544</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 2.5 μg/mL in cell type 721_B_lymphoblasts

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