EIF3B

Gene Symbol EIF3B
Entrez Gene 8662
Alt Symbol EIF3-ETA, EIF3-P110, EIF3-P116, EIF3S9, PRT1
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 3, subunit B
Other Description eIF-3-eta|eIF3 p110|eIF3 p116|eukaryotic translation initiation factor 3 subunit 9|eukaryotic translation initiation factor 3 subunit B|eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD)|eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa|hPrt1|prt1 homolog
Swissprots A4D208 Q9UMF9 Q2NL77 P55884
Accessions AAP21880 EAL23951 EAL23952 EAW87237 EAW87238 EAW87239 EAW87240 EAW87241 P55884 AB209381 BAD92618 AI888128 AK093807 AK126670 AK127885 AK300967 BAG62591 AK302058 BAG63448 BC001173 AAH01173 BC009986 AAH09986 BC110865 AAI10866 BG325094 BM556863 BQ015102 BQ045103 BQ070291 U62583 AAB42010 U78525 AAC99479 XM_011515599 XP_011513901 XM_011515600 XP_011513902 XM_011515601 XP_011513903 NM_001037283 NP_001032360 NM_003751 NP_003742
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre- initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. {ECO:0000269|PubMed:9388245}.
Subcellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03001}.
Top Pathways RNA transport

eIF3η (C-5) - sc-137214 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eIF3η (C-5): sc-137214. Western blot analysis of eIF3η expression in non-transfected: sc-117752 (A) and mouse eIF3η transfected: sc-119982 (B) 293T whole cell lysates and A-431 (C) and Jurkat (D) nuclear extracts.

eIF3η (A-20) - sc-16378 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF3η (A-20): sc-16378. Western blot analysis of eIF3η expression in A-431 (A) and Jurkat (B) nuclear extracts.

eIF3η (D-9) - sc-137215 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
eIF3η (D-9): sc-137215. Western blot analysis of eIF3η expression in non-transfected: sc-117752 (A) and mouse eIF3η transfected: sc-119982 (B) 293T whole cell lysates and A-431 (C) and Jurkat (D) nuclear extracts.

eIF3η (H-300) - sc-28857 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
eIF3η (H-300): sc-28857. Western blot analysis of eIF3η expression in non-transfected: sc-117752 (A) and human eIF3η transfected: sc-112106 (B) 293T whole cell lysates.

eIF3η (N-20) - sc-16377 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF3η (N-20): sc-16377. Western blot analysis of eIF3η expression in WEHI-231 (A), RAW 264.7 (B) and MM-142 (C) nuclear extracts.

Anti-eIF3B antibody - ab113218 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab113218 at a 1/100 dilution staining eIF3B in Human ovarian carcinoma by immunohistochemistry (FFPE section). Secondary antibody used was Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed DyLight® 594 Conjugated at a dilution of 1/100.

Anti-eIF3B antibody - ab166807 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lanes 1 - 2 : Anti-eIF3B antibody (ab166807) at 0.25 µg/mlLane 3 : Anti-eIF3B antibody (ab166807) at 0.05 µg/mlLane 1 : A431 cell lysateLane 2 : A431 cell lysate with immunizing peptideLane 3 : NIH3T3 cell lysate

Anti-eIF3B antibody [EPR5804] - ab133601 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5804
All lanes : Anti-eIF3B antibody [EPR5804] (ab133601) at 1/10000 dilutionLane 1 : A431 cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLane 5 : Caco 2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-eIF3B antibody [EPR5805] - ab124778 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5805
All lanes : Anti-eIF3B antibody [EPR5805] (ab124778) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : Caco 2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit at 1/2000 dilution

Anti-EIF3B Antibody (aa1-50) - LS-C125518 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF3B Antibody (aa100-150) - LS-C125519 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF3B Antibody (aa121-137) - LS-C148962 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-EIF3B Antibody (C-Terminus) - LS-C30323 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
A: Marker, B: 293T Cell Lysate

Anti-EIF3B Antibody (aa1-50) - LS-C288541 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EIF3B Antibody (Internal, clone EPR5804) - LS-C139003 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5804
Western blot analysis on (A) A431, (B) 293T, (C) Jurkat, (D) HeLa, and (E) Caco-2 cell lysates using anti-eIF-3B antibody.

Anti-EIF3B Antibody (Internal, clone EPR5805) - LS-C138931 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5805
IHC of paraffin-embedded human colon tissue using anti-eIF-3B antibody.

Anti-EIF3B Antibody (aa100-150) - LS-C288542 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human EIF3S9/eIF3B by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-EIF3S9/eIF3B used at a dilution of 1:200 (1 Detection: DAB.

Anti-EIF3B Antibody (aa755-775) - LS-C313156 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB of EIF3B antibody. Lane 1: HELA Cell Lysate. Lane 2: 293T Cell Lysate. Lane 3: A431 Cell Lysate..

Anti-EIF3B Antibody (aa1-50) IHC-plus™ - LS-B7956 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-EIF3B antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7956 dilution 10 ug/ml.

Anti-EIF3B Antibody IHC-plus™ - LS-B1990 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-EIF3B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1990 dilution 1:50.

Anti-EIF3B Antibody (aa100-150) - LS-C286866 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Detection of Mouse eIF3B/EIF3S9 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-eIF3B/EIF3S9 used at a dilution of 1:100.

EIF3B Antibody - NBP2-24571 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal

EIF3B Antibody - NB100-93302 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3B Antibody [NB100-93302] - Detection of Human EIF3S9/eIF3B on HeLa whole cell lysate using NB100-93302. EIF3S9/eIF3B was also immunoprecipitated by rabbit anti-EIF3S9/eIF3B antibody NB100-93301.

EIF3B Antibody - NBP2-38597 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3B Antibody [NBP2-38597] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

EIF3B Antibody - NB100-93301 from Novus Biologicals

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: EIF3B Antibody [NB100-93301] - Detection of Human and Mouse eIF3B/EIF3S9 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibody: Affinity purified rabbit antieIF3B/ EIF3S9 antibody NB100-93301 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF3B/EIF3S9 was immunoprecipitated efficiently by rabbit antieIF3B/ EIF3S9 antibody NB100-93302, which recognizes a downstream epitope.
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