CELE_F36A4.7

Gene Symbol CELE_F36A4.7
Entrez Gene 177190
Alt Symbol -
Species C. elegans
Gene Type protein-coding
Description AMA-1
Other Description -
Swissprots P16356 Q20090
Accessions CCD69532 P16356 NM_068122 NP_500523
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA h
Subcellular Location Nucleus {ECO:0000250}.
Top Pathways Purine metabolism, RNA polymerase, Pyrimidine metabolism

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody - ChIP Grade - ab5095 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC - Wholemount of Caenorhabditis elegans larvae labelling RNA polymerase II CTD repeat YSPTSPS (phospho S2) with ab5095. The sample was incubated with primary antibody (1/500 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, an goat anti-rabbit Alexa Fluor® 488 (1/1000), was used as the secondary antibody.See Abreview

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - ChIP Grade - ab5408 from Abcam

Prices $404.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4H8
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab5408 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody - ChIP Grade - ab5131 from Abcam

Prices $403.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
IHC - Wholemount of Caenorhabditis elegans larvae labelling RNA polymerase II CTD repeat YSPTSPS (phospho S5) with ab5131. The sample was incubated with primary antibody (1/500 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 - (1/1000), was used as the secondary antibody.See Abreview

Anti-RNA polymerase II CTD repeat YSPTSPS antibody [8WG16] - ChIP Grade - ab817 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8WG16
Various regions across the Actin2/7 loci were tested for the presence of RNA polymerase II CTD repeat YSPTSPS. A nuclear lysate from Arabidopsis thaliana seedlings was crosslinked using 1% formaldehyde for 30 seconds.  The ChIP was performed with 0.1 µg of ab817 per µg of chromatin; incubated together for 16 hours at 4°C.  The immunoprecipitated DNA was quantified by Real-Time PCR.  The bottom panel indicates the positive (ab817) and negative controls (no antibody) at region B3. See Abreview

ama-1 Antibody - 38520002 from Novus Biologicals

Prices $529.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: ama-1 Antibody [38520002]:This image is specific to animal number SDQ2357 Dilution: 1:10000

Anti-ama-1 monoclonal antibody, clone Sc2 - CABT-BL267 from Creative Diagnostics

Host Mouse
Clonality Monoclonal
Clone Sc2
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