DBT

Gene Symbol DBT
Entrez Gene 1629
Alt Symbol BCATE2, BCKAD-E2, BCKADE2, BCOADC-E2, E2, E2B
Species Human
Gene Type protein-coding
Description dihydrolipoamide branched chain transacylase E2
Other Description 52 kDa mitochondrial autoantigen of primary biliary cirrhosis|BCKAD E2 subunit|E2 component of branched chain alpha-keto acid dehydrogenase complex|branched chain 2-oxo-acid dehydrogenase complex component E2|branched chain acyltransferase, E2 component|branched-chain alpha-keto acid dehydrogenase complex component E2|dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex|dihydrolipoyl transacylase|dihydrolipoyllysine-residue (2-methylpropanoyl)transferase|lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial|lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex|mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b)
Swissprots B2R811 P11182 Q5VVL8
Accessions CAA48225 EAW72963 P11182 AK024946 AK303941 BAG64869 AK313191 BAG36008 BC016675 AAH16675 BC030002 BT007372 AAP36036 DA274670 J03208 AAA35589 M19301 AAA59200 M27093 AAA64512 X57677 X66785 CAA47285 XM_005270545 XP_005270602 XM_005270546 XP_005270603 XR_946560 NM_001918 NP_001909
Function The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
Subcellular Location Mitochondrion matrix.
Top Pathways Valine, leucine and isoleucine degradation

DBT (C-15) - sc-160269 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-DBT antibody - ab88471 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-DBT antibody (ab88471) at 1 µg/ml + Human liver tissue lysate at 50 µgSecondaryGoat Anti-Mouse IgG at 1/5000 dilution

Anti-DBT / E2 Antibody - LS-C307179 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-DBT / E2 Antibody (aa35-84) IHC-plus™ - LS-B4677 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-DBT / E2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4677 concentration 5 ug/ml.

Anti-DBT / E2 Antibody - LS-C310084 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DBT / E2 Antibody (HRP) - LS-C318563 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DBT / E2 Antibody (Biotin) - LS-C319085 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DBT / E2 Antibody - LS-C318564 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DBT / E2 Antibody (FITC) - LS-C319087 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DBT / E2 Antibody (aa83-132) - LS-C201598 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human Fetal Kidney. DBT antibody dilution 1.0 ug/ml.

Anti-DBT / E2 Antibody (clone 1G2) - LS-C173946 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.

DBT Antibody - NBP1-89522 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows positivity in mitochondria.

DBT Antibody - NBP1-31760 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DBT Antibody [NBP1-31760] - A. 50 ug mouse liver lysate/extract 10% SDS-PAGE DBT antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

DBT Antibody - NBP1-85964 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DBT Antibody [NBP1-85964] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

DBT Antibody - NBP1-85963 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DBT Antibody [NBP1-85963] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

DBT Antibody (1G2) - NBP2-02042 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G2
Western Blot: DBT Antibody (1G2) [NBP2-02042] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.

DBT Antibody - H00001629-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

DBT Antibody - H00001629-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DBT antibody produced in rabbit - HPA026481 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DBT: <B>Cat. No. HPA026481</B>: Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.

Anti-DBT antibody produced in rabbit - HPA026485 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DBT: <B>Cat. No. HPA026485</B>: Immunohistochemical staining of human rectum shows strong granular positivity in glandular cells.

Anti-DBT antibody produced in rabbit - HPA026533 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DBT: <B>Cat. No. HPA026533</B>: Immunohistochemical staining of human testis shows strong cytoplasmic positivity in leydig cells.

Anti-DBT, (N-terminal) antibody produced in rabbit - SAB2104091 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-DBT, antibody produced in rabbit: <B>Cat. No. SAB2104091</B>: Immunoblotting analysis of DBT in HT1080 cell lysate with DBT anitbody at 1 μg/mL.

Anti-DBT (246-260) antibody produced in rabbit - D6570 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-DBT (246-260), <B>Cat. No. D6570</B>: Immunofluorescence of HUVEC cells using Anti-DBT (246-260), D6570 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-DBT antibody produced in mouse - SAB1400071 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-DBT, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1400071</B>: Antibody concentration: 1.0 ~ 5.0 μg/mL. Specific band of ~53.487 kDa using human liver lysate.

17-beta-Estradiol Antibody (OAMA00648) - OAMA00648 from Aviva Systems Biology

Prices $455.00
Sizes 1 mg
Host Mouse
Clonality Monoclonal
Clone ESTR-1
Search more