MED4

Gene Symbol MED4
Entrez Gene 29079
Alt Symbol ARC36, DRIP36, HSPC126, TRAP36, VDRIP
Species Human
Gene Type protein-coding
Description mediator complex subunit 4
Other Description TRAP/SMCC/PC2 subunit p36|activator-recruited cofactor 36 kDa component|mediator of RNA polymerase II transcription subunit 4|mediator, 34-kD subunit, homolog|vitamin D receptor-interacting protein, 36-kD|vitamin D3 receptor-interacting protein complex 36 kDa component
Swissprots Q9BS95 Q9NPJ6 Q6IA79 Q5T912 Q53GB4 Q53H68 Q9NYR5 Q6FHC4 B4DX67
Accessions EAX08783 EAX08784 EAX08785 EAX08786 Q9NPJ6 AF161475 AAF29090 AF230381 AAF37289 AF300618 AAG22542 AI613470 AK001934 BAA91987 AK222713 BAD96433 AK223017 BAD96737 AK301835 BAG63279 BC005189 AAH05189 BP309440 CR457276 CAG33557 CR541830 CAG46629 DQ893076 ABM84002 DQ896333 ABM87332 HG518167 CDI06890 NM_001270629 NP_001257558 NM_014166 NP_054885
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Subcellular Location Nucleus.

Med4 (D-6) - sc-398179 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Med4 (D-6): sc-398179. Western blot analysis of Med4 expression in NTERA-2 cl.D1 (A), Jurkat (B), Hep G2 (C) and K-562 (D) whole cell lysates.

Med4 (D-7) - sc-398180 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Med4 (D-7): sc-398180. Western blot analysis of Med4 expression in NTERA-2 cl.D1 (A), Jurkat (B), Hep G2 (C) and K-562 (D) whole cell lysates.

Med4 (C-16) - sc-84198 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Med4 (C-16): sc-84198. Western blot analysis of Med4 expression in 293T whole cell lysate.

Med4 (FL-270) - sc-292835 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Med4 (FL-270): sc-292835. Western blot analysis of Med4 expression in NTERA-2 cl.D1 whole cell lysate.

Med4 (G-18) - sc-84200 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Med4 (A-24) - sc-133779 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
Med4 (A-24): sc-133779. Western blot analysis of Med4 expression in Hep G2 whole cell lysate.

Anti-MED4 antibody - ab113706 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MED4 antibody (ab113706) at 0.5 µg/ml + Human testis tissue lysate

Anti-MED4 antibody [EPR7816] - ab129170 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7816
All lanes : Anti-MED4 antibody [EPR7816] (ab129170) at 1/1000 dilutionLane 1 : HepG2 cell lysatesLane 2 : T47D cell lysatesLane 3 : K562 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Anti-MED4 antibody - N-terminal - ab153962 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MED4 antibody - N-terminal (ab153962) at 1/1000 dilution + A549 whole cell lysate at 30 µg

Anti-MED4 Antibody (aa146-195) IHC-plus™ - LS-B3562 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MED4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3562 concentration 5 ug/ml.

Anti-MED4 Antibody - LS-C308941 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-MED4 Antibody (C-Terminus) - LS-C81576 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.

Anti-MED4 Antibody (C-Terminus) IHC-plus™ - LS-B4986 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MED4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4986 concentration 5 ug/ml.

Anti-MED4 Antibody (Internal, clone EPR7816) - LS-C152507 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7816
IHC of paraffin embedded human colon tissue using anti-MED4 antibody.

MED4 Antibody - AF5089 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human MED4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5089) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MED4 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

MED4 Antibody - NBP1-84977 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

MED4 Antibody - NBP1-76930 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: MED4 Antibody [NBP1-76930] - Human testis tissue lysate with MED4 antibody at 0.5 ug/mL.

MED4 Antibody - NBP1-33671 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MED4 Antibody [NBP1-33671] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE; antibody diluted at 1:1000.

MED4 Antibody (2B10) - H00029079-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B10
Western Blot: MED4 Antibody (2B10) [H00029079-M01] - Analysis of MED4 expression in transfected 293T cell line by MED4 monoclonal antibody (M01), clone 2B10.Lane 1: MED4 transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.

Anti-MED4 antibody produced in rabbit - HPA006232 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MED4: <B>Cat. No. HPA006232</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placental tissue showing nuclear staining of trophoblastic and decidual cells.

Monoclonal Anti-MED4 antibody produced in mouse - SAB1402571 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B10
<B>Immunoblotting</B><BR/>Monoclonal Anti-MED4, antibody produced in mouse, clone 2B10, purified immunoglobulin: <B>Cat. No. SAB1402571</B>: Antibody concentration: 1 μg/mL. Specific band of ~55.81 kDa using immunogen protein lysate.

Anti-MED4 (AB1) antibody produced in rabbit - AV50561 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MED4 (ab1): <B>Cat. No. AV50561</B>: Immunohistochemistry of MED4 in Human Testis tissue with MED4 antibody at 5.0 μg/mL.

Anti-MED4 (AB2) antibody produced in rabbit - AV50562 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: HepG2 (<B>Cat. No. AV50562</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.5 μg/mL in cell type HepG2

Anti-MED4 antibody produced in rabbit - SAB3500264 from Sigma-Aldrich

Prices $348.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-MED4: <B>Cat. No. SAB3500264</B>: Immunohistochemistry of MED4 in human testis tissue with MED4 antibody at 10 μg/mL.

MED4 antibody - middle region (ARP50561_P050) - ARP50561_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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