Gene Symbol | SPRTN |
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Entrez Gene | 83932 |
Alt Symbol | C1orf124, DVC1, PRO4323, spartan |
Species | Human |
Gene Type | protein-coding |
Description | SprT-like N-terminal domain |
Other Description | DNA damage protein targeting VCP|DNA damage-targeting VCP (p97) adaptor|sprT-like domain-containing protein Spartan|zinc finger RAD18 domain-containing protein |
Swissprots | Q6UWW6 Q9H040 Q96KA0 Q5TE78 B5MEF7 B1AKT0 Q96BC5 |
Accessions | CCQ43030 CCQ43757 EAW69956 EAW69957 EAW69958 Q9H040 AK027317 BAB55037 AK027613 BAB55232 AL512744 CAC21670 AM393399 CAL38277 AM393461 CAL38338 AM393630 CAL38506 AY358611 AAQ88974 BC015740 AAH15740 BC068478 AAH68478 BM480135 BU630543 XM_005273284 XP_005273341 XM_005273285 XP_005273342 XM_006711818 XP_006711881 XM_011544288 XP_011542590 XM_011544289 XP_011542591 NM_001010984 NP_001010984 NM_001261462 NP_001248391 NM_032018 NP_114407 |
Function | Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. {ECO:0000269|PubMed:22681887, ECO:0000269|PubMed:22894931, ECO:0000269|PubMed:22902628, ECO:0000269|PubMed:22987070, ECO:0000269|PubMed:23042605, ECO:0000269|PubMed:23042607}. |
Subcellular Location | Nucleus. Chromosome. Note=Localizes to sites of UV damage via the PIP-box. Recruited to stalled relication forks at sites of replication stress. |
Spartan CRISPR/Cas9 KO Plasmid (h) - sc-413524 from Santa Cruz Biotechnology
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Spartan HDR Plasmid (h) - sc-413524-HDR from Santa Cruz Biotechnology
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Spartan Double Nickase Plasmid (h) - sc-413524-NIC from Santa Cruz Biotechnology
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Spartan Double Nickase Plasmid (h2) - sc-413524-NIC-2 from Santa Cruz Biotechnology
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Spartan CRISPR Activation Plasmid (h) - sc-413524-ACT from Santa Cruz Biotechnology
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Spartan CRISPR Activation Plasmid (h2) - sc-413524-ACT-2 from Santa Cruz Biotechnology
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Spartan Lentiviral Activation Particles (h) - sc-413524-LAC from Santa Cruz Biotechnology
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Spartan Lentiviral Activation Particles (h2) - sc-413524-LAC-2 from Santa Cruz Biotechnology
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Spartan siRNA (h) - sc-88266 from Santa Cruz Biotechnology
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Spartan shRNA Plasmid (h) - sc-88266-SH from Santa Cruz Biotechnology
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Spartan shRNA (h) Lentiviral Particles - sc-88266-V from Santa Cruz Biotechnology
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C1orf124 RNAi - H00083932-R01 from Novus Biologicals
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C1orf124 RNAi - H00083932-R02 from Novus Biologicals
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MISSION® esiRNA - EHU087351 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN4586 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND4586 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR11704 from Sigma-Aldrich
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SPRTN siRNA (Human) - CRJ3242 from Cohesion Biosciences
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SPRTN - MBS8212502 from MyBioSource
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shRNA set against Human C1orf124 (NM_032018.5) - SHH250362 from Creative biogene
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Human SPRTN siRNA - orb273743 from Biorbyt
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SPRTN shRNA Plasmid (Human) - CHJ3242 from Cohesion Biosciences
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SPRTN miRNA 3'UTR clone - MiUTR3H-14047 from Creative biogene
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