Gene Symbol | NEDD8 |
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Entrez Gene | 4738 |
Alt Symbol | NEDD-8 |
Species | Human |
Gene Type | protein-coding |
Description | neural precursor cell expressed, developmentally down-regulated 8 |
Other Description | neddylin|ubiquitin-like protein Nedd8 |
Swissprots | Q6LES6 Q3SXN8 Q15843 |
Accessions | CDM22114 Q15843 AK125214 BC038290 BC064899 BC104200 AAI04201 BC104201 AAI04202 BC104664 AAI04665 BE548741 CF130271 CR407662 CAG28590 D23662 BAA04889 NM_006156 NP_006147 |
Function | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. {ECO:0000269|PubMed:10318914, ECO:0000269|PubMed:10597293, ECO:0000269|PubMed:11953428}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:9353319}. Note=Mainly nuclear. |
Tissue Specificity | Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. {ECO:0000269|PubMed:10597293, ECO:0000269|PubMed:9353319}. |
NEDD8 CRISPR/Cas9 KO Plasmid (h) - sc-417924 from Santa Cruz Biotechnology
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NEDD8 HDR Plasmid (h) - sc-417924-HDR from Santa Cruz Biotechnology
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NEDD8 Double Nickase Plasmid (h) - sc-417924-NIC from Santa Cruz Biotechnology
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NEDD8 Double Nickase Plasmid (h2) - sc-417924-NIC-2 from Santa Cruz Biotechnology
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NEDD8 CRISPR Activation Plasmid (h) - sc-417924-ACT from Santa Cruz Biotechnology
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NEDD8 CRISPR Activation Plasmid (h2) - sc-417924-ACT-2 from Santa Cruz Biotechnology
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NEDD8 Lentiviral Activation Particles (h) - sc-417924-LAC from Santa Cruz Biotechnology
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NEDD8 Lentiviral Activation Particles (h2) - sc-417924-LAC-2 from Santa Cruz Biotechnology
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NEDD8 siRNA (h) - sc-36026 from Santa Cruz Biotechnology
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NEDD8 shRNA Plasmid (h) - sc-36026-SH from Santa Cruz Biotechnology
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NEDD8 shRNA (h) Lentiviral Particles - sc-36026-V from Santa Cruz Biotechnology
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NEDD8 RNAi - H00004738-R01 from Novus Biologicals
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MISSION® esiRNA - EHU112521 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN15188 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15188 from Sigma-Aldrich
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NEDD8 siRNA (Human) - CRH3147 from Cohesion Biosciences
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Human NEDD8 siRNA - orb263649 from Biorbyt
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NEDD8 shRNA Plasmid (Human) - CHH3147 from Cohesion Biosciences
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NEDD8 miRNA 3'UTR clone - MiUTR1H-06711 from Creative biogene
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