CHST12

Gene Symbol CHST12
Entrez Gene 55501
Alt Symbol C4S-2, C4ST-2, C4ST2
Species Human
Gene Type protein-coding
Description carbohydrate (chondroitin 4) sulfotransferase 12
Other Description carbohydrate sulfotransferase 12|chondroitin 4-O-sulfotransferase 2|chondroitin 4-sulfotransferase 2|sulfotransferase Hlo
Swissprots Q9NXY7 Q9NRB3 A4D1Z9 Q502W3
Accessions EAL23955 EAW87243 EAW87244 EAW87245 Q9NRB3 AF239822 AAF81692 AJ289131 CAB92133 AK223191 BAD96911 AK223211 BAD96931 AK313484 BAG36268 AY358574 AAQ88937 BC002918 AAH02918 BC015954 BC095492 AAH95492 DA078292 DA704587 DQ892969 ABM83895 DQ896217 ABM87216 XM_011515443 XP_011513745 XM_011515444 XP_011513746 NM_001243794 NP_001230723 NM_001243795 NP_001230724 NM_018641 NP_061111
Function Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.
Subcellular Location Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.
Tissue Specificity Widely expressed. Expressed a high level in spinal chord, heart, spleen, thyroid, pituitary gland, adrenal gland, peripheral blood leukocytes, thymus, lung, small intestine, fetal kidney, fetal spleen and fetal lung. {ECO:0000269|PubMed:10781601}.
Top Pathways Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate

C4ST-2 (D-20) - sc-68011 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CHST12 (D-20): sc-68011. Western blot analysis of CHST12 expression in TE 671 whole cell lysate.

Anti-CHST12 antibody - ab68734 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-CHST12 antibody (ab68734) at 1/500 dilutionLane 1 : CHST12 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

Anti-CHST12 Antibody - LS-C307791 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-CHST12 Antibody (aa56-151) - LS-C307794 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

CHST12 Antibody - NBP1-91784 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CHST12 Antibody [NBP1-91784] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4

CHST12 Antibody - H00055501-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: CHST12 Antibody [H00055501-B01P] - Analysis of CHST12 expression in transfected 293T cell line by CHST12 polyclonal antibody.  Lane 1: CHST12 transfected lysate(45.54 KDa). Lane 2: Non-transfected lysate.

CHST12 Antibody (3D6) - H00055501-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3D6
Western Blot: CHST12 Antibody (3D6) [H00055501-M01] - Analysis of CHST12 expression in transfected 293T cell line by CHST12 monoclonal antibody (M01), clone 3D6.Lane 1: CHST12 transfected lysate (Predicted MW: 48.4 KDa).Lane 2: Non-transfected lysate.

Anti-CHST12 antibody produced in rabbit - HPA041680 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CHST12: <B>Cat. No. HPA041680</B>: Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in glandular cells.

Anti-CHST12 antibody produced in mouse - SAB1407666 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CHST12: <B>Cat. No. SAB1407666</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~45.5 kDa using CHST12 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Monoclonal Anti-CHST12 antibody produced in mouse - SAB1403291 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3D6
<B>Immunoblotting</B><BR/>Monoclonal Anti-CHST12: <B>Cat. No. SAB1403291</B>: Antibody concentration: 1 μg/mL. Specific band of ~36.6 kDa using immunogen protein lysate.

CHST12-Antibody-N-term - AP21028b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CHST12 Antibody (N-term) at 1:2000 dilution + HL-60 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

CHST12 antibody - 70R-16414 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

CarbohydRate (Chondroitin 4) Sulfotransferase 12 Antibody - abx111364 from Abbexa

Prices $490.00, $490.00, $490.00, $490.00
Sizes 10 μl, 50 μl, 100 μl, 200 μl
Host Rabbit
Clonality Polyclonal

CHST12 antibody - 15341-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical of  paraffin-embedded human colon cancer using 15341-1-AP(CHST12 antibody) at dilution of 1:50 (under 10x lens)

CHST12 antibody - H00055501-B01P from Acris Antibodies

Prices $360.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot analysis of CHST12 expression in transfected 293T cell line (H00055501-T01) by CHST12 MaxPab polyclonal antibody.  Lane 1: CHST12 transfected lysate(45.54 KDa). Lane 2: Non-transfected lysate.

CHST12 antibody - H00055501-M01 from Acris Antibodies

Prices $360.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3D6

CHST12 antibody - 15341-1-AP from Proteintech Group

Prices $279.00
Sizes con: 25 μg/150μl
Host Rabbit
Clonality Polyclonal
K-562 cells were subjected to SDS PAGE followed by western blot with 15341-1-AP(CHST12 antibody) at dilution of 1:1000

CHST12, Polyclonal Antibody - MBS6008107 from MyBioSource

Prices $580.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
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