Gene Symbol | Msh2 |
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Entrez Gene | 17685 |
Alt Symbol | AI788990 |
Species | Mouse |
Gene Type | protein-coding |
Description | mutS homolog 2 (E. coli) |
Other Description | DNA mismatch repair protein Msh2|mutS protein homolog 2 |
Swissprots | P43247 |
Accessions | EDL38635 P43247 AK051960 AK078775 AK157842 BAE34223 AK166179 AK206893 AK216255 AK217675 BC047117 AAH47117 BC050897 AAH50897 U21011 AAA75027 X81143 CAA57049 NM_008628 NP_032654 |
Function | Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformationa |
Subcellular Location | Nucleus {ECO:0000305}. |
Top Pathways | Mismatch repair, Colorectal cancer |
MSH2 CRISPR/Cas9 KO Plasmid (m) - sc-421715 from Santa Cruz Biotechnology
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MSH2 HDR Plasmid (m) - sc-421715-HDR from Santa Cruz Biotechnology
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MSH2 Double Nickase Plasmid (m) - sc-421715-NIC from Santa Cruz Biotechnology
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MSH2 Double Nickase Plasmid (m2) - sc-421715-NIC-2 from Santa Cruz Biotechnology
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MSH2 CRISPR Activation Plasmid (m) - sc-421715-ACT from Santa Cruz Biotechnology
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MSH2 CRISPR Activation Plasmid (m2) - sc-421715-ACT-2 from Santa Cruz Biotechnology
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MSH2 Lentiviral Activation Particles (m) - sc-421715-LAC from Santa Cruz Biotechnology
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MSH2 Lentiviral Activation Particles (m2) - sc-421715-LAC-2 from Santa Cruz Biotechnology
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MSH2 siRNA (m) - sc-35970 from Santa Cruz Biotechnology
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MSH2 shRNA Plasmid (m) - sc-35970-SH from Santa Cruz Biotechnology
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MSH2 shRNA (m) Lentiviral Particles - sc-35970-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN35878 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND35878 from Sigma-Aldrich
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MSH2 siRNA (Mouse) - CRM2598 from Cohesion Biosciences
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shRNA set against Mouse MSH2 (NM_008628.2) - SHH345620 from Creative biogene
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shRNA set against Mouse Msh2(NM_008628.2) - SHH203483 from Creative biogene
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MSH2 shRNA Plasmid (Mouse) - CHM2598 from Cohesion Biosciences
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MSH2 miRNA 3'UTR clone - MiUTR1M-07332 from Creative biogene
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