PYCARD

Gene Symbol	PYCARD
Entrez Gene	29108
Alt Symbol	ASC, CARD5, TMS, TMS-1, TMS1
Species	Human
Gene Type	protein-coding
Description	PYD and CARD domain containing
Other Description	apoptosis-associated speck-like protein containing a CARD|caspase recruitment domain-containing protein 5|target of methylation-induced silencing 1
Swissprots	Q9BSZ5 Q9NXJ8 Q96D12 Q9HBD0 Q9ULZ3
Accessions	AAG01187 EAW52148 EAW52149 Q9ULZ3 AB023416 BAA87339 AF184073 AAG01188 AF255794 AAF99665 AF310103 AAG30286 AF384665 AAK63850 AK000211 BAA91012 AK314422 BAG37041 BC004470 AAH04470 BC013569 AAH13569 JF432570 ADZ15787 NM_013258 NP_037390 NM_145182 NP_660183 NM_145183
Function	Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in macrophage pyroptosis, a caspase-1-dependent inflammatory form of cell death and is the major constituent of the ASC pyroptosome which forms upon potassium depletion and rapidly recruits and activates caspase-1. In innate immune response believed to act as an integral adapter in the assembly of the inflammasome which activates caspase-1 leading to processing and secretion of proinflammatory cytokines. The function as activating adapter in different types of inflammasomes is mediated by the DA
Subcellular Location	Cytoplasm. Endoplasmic reticulum. Mitochondrion. Nucleus. Note=Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. Upon NLRP3 inflammasome activation redistributes to the perinuclear space localizing to endoplasmic reticulum and mitochondria. Localized primarily to the nucleus in resting monocytes/macrophages and rapidly redistributed to the cytoplasm upon pathogen infection. Localized to large cytoplasmic aggregate appearing as a speck containing AIM2, PYCARD, CASP8 and bacterial DNA after infection with Francisella tularensis (By similarity). {ECO:0000250}.
Tissue Specificity	Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.
Top Pathways	NOD-like receptor signaling pathway, Cytosolic DNA-sensing pathway, Pertussis, Influenza A, Salmonella infection

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

Human TMS1 peptide - ab22875 from Abcam Category Peptide Species Human

Human TMS1 protein fragment - ab131786 from Abcam Category Protein Source Wheat germ Species Human

TMS1 peptide - ab8395 from Abcam Category Peptide

PYCARD / ASC Antibody Blocking Peptide - LS-E2765 from LifeSpan Bioscience Category Peptide Prices $145.00 Sizes 100 µg

PYCARD / ASC Antibody Blocking Peptide - LS-E25289 from LifeSpan Bioscience Category Peptide Prices $145.00 Sizes 100 µg

PYCARD / ASC Antibody Blocking Peptide - LS-E29937 from LifeSpan Bioscience Category Peptide Prices $145.00 Sizes 50 µg

Recombinant Human ASC/TMS1 Protein - H00029108-P01 from Novus Biologicals Category Protein Prices $369.00, $529.00 Sizes 10 µg, 25 µg Source Wheat Germ Species Human

ASC/TMS1 Blocking Peptide - NBP1-77297PEP from Novus Biologicals Category Peptide Prices $129.00 Sizes 50 µg Species Human

PYCARD-Antibody-C-term-Blocking-peptide - BP13862b from Abgent, a WuXi AppTec company Category Peptide Prices $80.00 Sizes 100 µg

TMS1 blocking peptide - GTX22236-PEP from GeneTex Category Peptide Prices $239.00 Sizes 100 μg Species Human

TMS1 Peptide - AC21-0117-P from Abcore Category Peptide Prices $160.00 Sizes 100 µg

TMS1 Peptide - AC21-2952-P from Abcore Category Peptide Prices $160.00 Sizes 100 µg

CARD5 / PYCARD / ASC - H00029108-P01-10 from Acris Antibodies Category Protein Prices $380.00 Sizes 10 µg Source in vitro transl. Species Human

CARD5 / PYCARD / ASC - H00029108-P01-25 from Acris Antibodies Category Protein Prices $500.00 Sizes 25 µg Source in vitro transl. Species Human

CARD5 / PYCARD / ASC Control Peptide - AP30084CP-N from Acris Antibodies Category Peptide Prices $135.00 Sizes 50 µg

ASC Peptide - 2287P from ProSci Category Peptide Species Human

PYCARD Peptide - 45-129P from ProSci Category Peptide Prices $175.00 Sizes 100 µg Species Human

TMS1 fusion protein - Ag0774 from Proteintech Group Category Protein Prices $199.00 Sizes 50 μg Source E. coli -derived, PGEX-4T Species Human

Recombinant Human PYCARD, GST-tagged - PYCARD-29108H from Creative Biomart Category Protein Source Wheat Germ Species Human

Recombinant Human TMS1, GST-tagged - TMS1-3309H from Creative Biomart Category Protein Source E.coli Species Human

TMS1 (E13) Peptide - BS2215P from Bioworld Technology Category Peptide Prices $158.00 Sizes 100 µl

Recombinant Human Apoptosis-associated speck-like protein containing a CARD(PYCARD) - CSB-YP890936HU CSB-EP890936HU CSB-BP890936HU CSB-MP890936HU from Cusabio Category Protein Source Yeast or E.coli or Baculovirus or Mammalian cell Species Human

CARD5 / PYCARD / ASC (transcript variant 1) - TP315592 from Acris Antibodies Category Protein Prices $680.00 Sizes 20 µg Source HEK293 cells Species Human

CARD5 / PYCARD / ASC (transcript variant 2) - TP324051 from Acris Antibodies Category Protein Prices $680.00 Sizes 20 µg Source HEK293 cells Species Human

Apoptosis-associated speck-like protein containing a CARD (PYCARD) , Recombinant Protein - MBS1316599 from MyBioSource Category Protein Prices $1,405.00, $1,405.00, $1,870.00, $1,870.00 Sizes 1 mg (E-Coli), 100 µg (Baculovirus), 1 mg (Yeast), 100 µg (Mammalian-Cell) Source E Coli or Yeast or Baculovirus or Mammalian Cell Species Human

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