Gene Symbol | KNTC1 |
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Entrez Gene | 9735 |
Alt Symbol | ROD |
Species | Human |
Gene Type | protein-coding |
Description | kinetochore associated 1 |
Other Description | Rough Deal homolog, centromere/kinetochore protein|kinetochore-associated protein 1 |
Swissprots | B3KSG2 A7E2C4 P50748 |
Accessions | EAW98333 EAW98334 P50748 AA157787 AI916095 AK093470 BAG52724 AK293403 BAG56910 AK303337 BAG64400 BC055410 BC150278 AAI50279 D79988 BAA11483 XM_006719706 XP_006719769 XM_011539027 XP_011537329 XM_011539028 XP_011537330 XM_011539029 XP_011537331 XM_011539030 XP_011537332 XR_944844 XR_944845 XR_944846 NM_014708 NP_055523 |
Function | Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. {ECO:0000269|PubMed:11146660, ECO:0000269|PubMed:11590237, ECO:0000269|PubMed:15824131}. |
Subcellular Location | Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Dynamic pattern of localization during the cell cycle. At interphase, uniformly distributed throughout the cytoplasm and nucleus. By prophase and until late stages of prometaphase, a fraction of the total pool is concentrated at kinetochores. By metaphase, detected at kinetochores, along spindle fibers and most prominently at the poles. By late anaphase until the end of telophase, no longer detectable on kinetochores or along spindle fibers, but still present at the spindle poles. |
Tissue Specificity | High expression in testis. |
ROD CRISPR/Cas9 KO Plasmid (h) - sc-405696 from Santa Cruz Biotechnology
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ROD HDR Plasmid (h) - sc-405696-HDR from Santa Cruz Biotechnology
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ROD Double Nickase Plasmid (h) - sc-405696-NIC from Santa Cruz Biotechnology
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ROD Double Nickase Plasmid (h2) - sc-405696-NIC-2 from Santa Cruz Biotechnology
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ROD CRISPR Activation Plasmid (h) - sc-405696-ACT from Santa Cruz Biotechnology
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ROD CRISPR Activation Plasmid (h2) - sc-405696-ACT-2 from Santa Cruz Biotechnology
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ROD Lentiviral Activation Particles (h) - sc-405696-LAC from Santa Cruz Biotechnology
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ROD Lentiviral Activation Particles (h2) - sc-405696-LAC-2 from Santa Cruz Biotechnology
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ROD siRNA (h) - sc-62958 from Santa Cruz Biotechnology
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ROD shRNA Plasmid (h) - sc-62958-SH from Santa Cruz Biotechnology
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ROD shRNA (h) Lentiviral Particles - sc-62958-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU044431 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN11336 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND11336 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR00376 from Sigma-Aldrich
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KNTC1 siRNA (Human) - CRH6503 from Cohesion Biosciences
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KNTC1 RNAi - H00009735-R01 from Novus Biologicals
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shRNA set against Human KNTC1(NM_014708.4) - SHL204794 from Creative biogene
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Human KNTC1 siRNA - orb267005 from Biorbyt
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KNTC1 shRNA Plasmid (Human) - CHH6503 from Cohesion Biosciences
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KNTC1 miRNA 3'UTR clone - MiUTR1H-05422 from Creative biogene
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