Gene Symbol | RAD51 |
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Entrez Gene | 5888 |
Alt Symbol | BRCC5, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA |
Species | Human |
Gene Type | protein-coding |
Description | RAD51 recombinase |
Other Description | BRCA1/BRCA2-containing complex, subunit 5|DNA repair protein RAD51 homolog 1|RAD51 homolog A|RecA, E. coli, homolog of|RecA-like protein|recombination protein A |
Swissprots | Q9BV60 B0FXP0 B2R8T6 Q6FHX9 Q6ZNA8 Q06609 |
Accessions | AAD49705 AAF61901 AAF69145 AAN87149 EAW92431 EAW92432 EAW92433 EAW92434 EAW92435 Q06609 AK131299 BAD18467 AK291969 BAF84658 AK303089 BAG64200 AK313503 BAG36283 AU100170 AY425955 AAR07948 AY608888 AAT37157 BC001459 AAH01459 BT019705 AAV38511 CR536559 CAG38796 D13804 BAA02962 D14134 BAA03189 EU362635 ABY59731 XM_006720626 XP_006720689 XM_011521857 XP_011520159 XM_011521858 XP_011520160 XM_011521859 XP_011520161 XM_011521860 XP_011520162 XM_011521861 XP_011520163 XM_011521862 XP_011520164 NM_001164269 NP_001157741 NM_001164270 NP_001157742 NM_002875 NP_002866 NM_133487 NP_597994 |
Function | Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. {ECO:0000269|PubMed:12205100, ECO:0000269|PubMed:12442171, ECO:0000269|PubMed:18417535, ECO:0000269|PubMed:20231364, ECO:0000269|PubMed:20348101, ECO:0000269|PubMed:20413593, ECO:0000269|PubMed:23509288, ECO:0000269|PubMed:23754376}. |
Subcellular Location | Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner. |
Tissue Specificity | Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. |
Top Pathways | Homologous recombination, Pancreatic cancer, Fanconi anemia pathway |
Rad51 CRISPR/Cas9 KO Plasmid (h) - sc-400100 from Santa Cruz Biotechnology
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Rad51 HDR Plasmid (h) - sc-400100-HDR from Santa Cruz Biotechnology
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Rad51 Double Nickase Plasmid (h) - sc-400100-NIC from Santa Cruz Biotechnology
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Rad51 Double Nickase Plasmid (h2) - sc-400100-NIC-2 from Santa Cruz Biotechnology
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Rad51 CRISPR Activation Plasmid (h) - sc-400100-ACT from Santa Cruz Biotechnology
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Rad51 CRISPR Activation Plasmid (h2) - sc-400100-ACT-2 from Santa Cruz Biotechnology
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Rad51 Lentiviral Activation Particles (h) - sc-400100-LAC from Santa Cruz Biotechnology
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Rad51 Lentiviral Activation Particles (h2) - sc-400100-LAC-2 from Santa Cruz Biotechnology
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Rad51 siRNA (h) - sc-36361 from Santa Cruz Biotechnology
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Rad51 shRNA Plasmid (h) - sc-36361-SH from Santa Cruz Biotechnology
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Rad51 shRNA (h) Lentiviral Particles - sc-36361-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU045521 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1295 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1295 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR07520 from Sigma-Aldrich
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RAD51 siRNA (Human) - CRH3977 from Cohesion Biosciences
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Rad51 RNAi - H00005888-R03 from Novus Biologicals
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Rad51 RNAi - H00005888-R04 from Novus Biologicals
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RAD51 - MBS8233908 from MyBioSource
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shRNA set against Human RAD51 (NM_002875.4) - SHH388996 from Creative biogene
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shRNA set against Human RAD51(NM_001164270.1) - SHR161868 from Creative biogene
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Human RAD51 siRNA - orb264479 from Biorbyt
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RAD51 shRNA Plasmid (Human) - CHH3977 from Cohesion Biosciences
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RAD51 miRNA 3'UTR clone - MiUTR1H-08595 from Creative biogene
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RAD51 miRNA 3'UTR clone - MiUTR3H-01998 from Creative biogene
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