GAPDH

Gene Symbol GAPDH
Entrez Gene 374193
Alt Symbol KNC-NDS6
Species Chicken
Gene Type protein-coding
Description glyceraldehyde-3-phosphate dehydrogenase
Other Description peptidyl-cysteine S-nitrosylase GAPDH
Swissprots Q90848 Q98926 Q90849 P00356
Accessions AAA48774 P00356 AF047874 AAD02474 AY466166 BX934060 J00850 K01458 AAA48778 V00406 CAA23697 V00407 CAA23698 X01578 CAA25733 NM_204305 NP_989636
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm, cytosol {ECO:0000250}. Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}.
Top Pathways Glycolysis / Gluconeogenesis, Biosynthesis of amino acids, Carbon metabolism

Anti-GAPDH antibody - ab157156 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling GAPDH with ab157156 at 1/1000 (1µg/ml). Detection: DAB.

Anti-GAPDH antibody - ab70699 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling GAPDH with ab70699 at 1/200 (1µg/ml). Detection: DAB.

Anti-GAPDH antibody [EPR16884] - ab181603 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16884
All lanes : Anti-GAPDH antibody [EPR16884] (ab181603) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : MDBK (Bovine kidney cell line) whole cell lysatesLane 3 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysatesLane 4 : MDCK (Canine kidney cell line) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-GAPDH antibody [EPR16891] - ab181602 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16891
All lanes : Anti-GAPDH antibody [EPR16891] (ab181602) at 1/50000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : Xenopus(X. tropicalis) muscle lysatesLane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysatesLane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-GAPDH antibody [EPR16891] (Alexa Fluor® 488) - ab201768 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16891
ab201768 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201768 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-GAPDH antibody [EPR16891] (Alexa Fluor® 647) - ab201272 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16891
ab201272 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201272 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-GAPDH antibody [GA1R] - ab125247 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone GA1R
All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilutionLane 1 : Human tissue lysateLane 2 : Mouse tissue lysateLane 3 : Rat tissue lysateLane 4 : Rabbit tissue lysateLane 5 : Chicken tissue lysateLane 6 : Hamster tissue lysateLysates/proteins at 5 µg per lane.developed using the ECL technique

Anti-GAPDH antibody [GA1R] - ab127428 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone GA1R
All lanes : Anti-GAPDH antibody [GA1R] (ab127428) at 1/2000 dilutionLane 1 : Human tissue lysateLane 2 : Mouse tissue lysateLane 3 : Rat tissue lysateLane 4 : Rabbit tissue lysateLane 5 : Chicken tissue lysateLane 6 : Hamster tissue lysateLysates/proteins at 5 µg per lane.developed using the ECL technique

Anti-GAPDH antibody (HRP) - ab85760 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab85760 at 2.5µg/ml staining GAPDH in breast tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded section).

Anti-GAPDH antibody [mAbcam 9484] (Alexa Fluor® 647) - ab196697 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 9484
ab196697 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196697 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free - ab174646 from Abcam

Prices $372.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 9484

Anti-GAPDH antibody [mAbcam 9484] - Loading Control - ab9484 from Abcam

Prices $401.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 9484
All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/5000 dilutionLane 1 : Hela whole cell (Human)Lane 2 : 3T3 cell (Mouse)Lane 3 : Rat brainLane 4 : Xenopus embryoLane 5 : Chicken LiverLane 6 : EBTr cell (Cow)Lane 7 : CHO cell (Chinese hamster)Lane 8 : Pig liverSecondaryRabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilutionPerformed under reducing conditions.

Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) - ab9482 from Abcam

Prices $394.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 9484
Lanes 1 - 4 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482) at 1/5000 dilution ((Blocked in 5% MILK))Lanes 5 - 8 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP) (ab9482) at 1/5000 dilution ((Blocked in 5% BSA))Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-GAPDH antibody - Loading Control - ab9485 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab9485 staining GAPDH in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9485 at 5μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

Glyceraldehyde-3-phosphate dehydrogenase antibody (Biotin) - orb240503 from Biorbyt

Prices $400.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal

GAPDH Antibody (OACA00438) - OACA00438 from Aviva Systems Biology

Prices $270.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

GAPDH Antibody (OACA00439) - OACA00439 from Aviva Systems Biology

Prices $270.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

GAPDH Antibody (OACA00440) - OACA00440 from Aviva Systems Biology

Prices $270.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

GAPDH Antibody (OACA00441) - OACA00441 from Aviva Systems Biology

Prices $270.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Glyceraldehyde-3-phosphate dehydrogenase Antibody - abx110534 from Abbexa

Host Rabbit
Clonality Polyclonal

Glyceraldehyde-3-phosphate dehydrogenase Antibody (Biotin) - abx106254 from Abbexa

Host Rabbit
Clonality Polyclonal

Glyceraldehyde-3-phosphate dehydrogenase Antibody (FITC) - abx107668 from Abbexa

Host Rabbit
Clonality Polyclonal

Glyceraldehyde-3-phosphate dehydrogenase Antibody (HRP) - abx109085 from Abbexa

Host Rabbit
Clonality Polyclonal

Glyceraldehyde-3-phosphate dehydrogenase, Polyclonal Antibody - MBS1488931 from MyBioSource

Prices $160.00, $235.00
Sizes 50 µg, 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GAPDH monoclonal antibody, clone 2C3 - CABT-39089MC from Creative Diagnostics

Host Mouse
Clonality Monoclonal
Clone 2C3
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