HMGN1

Gene Symbol HMGN1
Entrez Gene 614915
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description high mobility group nucleosome binding domain 1
Other Description high mobility group nucleosome-binding domain-containing protein 1|high-mobility group nucleosome binding domain 1|high-mobility group nucleosome-binding domain-containing protein 1|non-histone chromosomal protein HMG-14
Swissprots Q3T0D1 P02316
Accessions DAA33053 P02316 BC102449 AAI02450 XM_010801622 XP_010799924 NM_001034772 NP_001029944
Function Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity). {ECO:0000250}.
Subcellular Location Nucleus.
Top Pathways Herpes simplex infection

Anti-HMG14 antibody - ab83184 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-HMG14 antibody (ab83184) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-HMG14 antibody - ChIP Grade - ab5212 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab5212 stained HeLa cells. The cells were 4% PFA fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5212 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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