TOPBP1

Gene Symbol TOPBP1
Entrez Gene 11073
Alt Symbol TOP2BP1
Species Human
Gene Type protein-coding
Description topoisomerase (DNA) II binding protein 1
Other Description DNA topoisomerase 2-binding protein 1|DNA topoisomerase II-beta-binding protein 1|DNA topoisomerase II-binding protein 1
Swissprots Q7LGC1 Q92547 B7Z7W8 Q9UEB9
Accessions EAW79173 EAW79174 EAW79175 Q92547 AB019397 BAA34202 AK026229 AK302584 BAH13754 BC017552 BC032633 AAH32633 BC126209 AAI26210 BC151237 AAI51238 D87448 BAA13389 Y18557 XM_005247076 XP_005247133 XM_011512357 XP_011510659 NM_007027 NP_008958
Function Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double- stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1- responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. {ECO:0000269|PubMed:10498869, ECO:0000269|PubMed:11395493, ECO:0000269|PubMed:11714696, ECO:0000269|PubMed:12697828, ECO:0000269|PubMed:15075294, ECO:0000269|PubMed:16530042}.
Subcellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Note=Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. {ECO:0000250}.
Tissue Specificity Highly expressed in heart, brain, placenta, lung and kidney. {ECO:0000269|PubMed:9461304}.

TopBP1 (D8G4L) Rabbit mAb - 14342 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8G4L
Western blot analysis of extracts from various cell types using TopBP1 (D8G4L) Rabbit mAb (upper) or α-Actinin (D6F6) XP ® Rabbit mAb (lower) #6487.

TopBP1 (B-7) - sc-271043 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TopBP1 (B-7): sc-271043. Western blot analysis of TopBP1 expression in Jurkat whole cell lysate.

TopBP1 (H-300) - sc-32923 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TopBP1 (H-300): sc-32923. Western blot analysis of TopBP1 expression in Jurkat nuclear extract.

TopBP1 (C-20) - sc-22859 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TopBP1 (C-20): sc-22859. Western blot analysis of TopBP1 expression in UV-treated HeLa whole cell lysate.

TopBP1 (L-20) - sc-22858 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TopBP1 (33) - sc-136106 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
TopBP1 (33): sc-136106. Western blot analysis of TopBP1 expression in Jurkat whole cell lysate.

Anti-TopBP1 antibody - ab105109 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TopBP1 antibody (ab105109) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 5 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-TopBP1 antibody - ab192304 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TopBP1 antibody - ChIP Grade - ab2402 from Abcam

Prices $401.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (aa1114-1142) - LS-C325868 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody - LS-C341868 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody - LS-C334288 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of A549 cell using TOPBP1 antibody. Blue: DAPI for nuclear staining.

Anti-TOPBP1 Antibody (C-Terminus) - LS-C287042 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse T op BP1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-TopBP1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-TOPBP1 Antibody - LS-C90609 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (aa1187-1621) - LS-C144235 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-TOPBP1 Antibody (aa1192-1621, clone 11n195) - LS-C184913 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 11n195

Anti-TOPBP1 Antibody (phospho-Ser1138, AP) - LS-C271951 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (phospho-Ser1138, Biotin) - LS-C271954 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (phospho-Ser1138, FITC) - LS-C271955 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (phospho-Ser1138, APC) - LS-C271953 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (phospho-Ser1159) - LS-C163681 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Dot blot of Phospho-TOPBP1-S1159 Antibody Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Anti-TOPBP1 Antibody (phospho-Ser1138, Azide-free, HRP) - LS-C271956 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (phospho-Ser1138, PE) - LS-C271957 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (phospho-Ser1159, AP) - LS-C271958 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TOPBP1 Antibody (phospho-Ser1159, Biotin) - LS-C271960 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more