HSP70.2

Gene Symbol HSP70.2
Entrez Gene 396648
Alt Symbol HSPA1A, HSPA1B
Species Pig
Gene Type protein-coding
Description heat shock protein 70.2
Other Description heat shock 70 kDa protein 1B|heat shock 70 kDa protein 2|heat shock 70kDa protein 1A|heat shock 70kDa protein 1B
Swissprots Q6S4N2
Accessions CAC35386 CAN13333 CAN87708 Q6S4N2 AY466608 AAR30953 NM_213766 NP_998931
Function In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. {ECO:0000250|UniProtKB:P08107}.
Subcellular Location Cytoplasm {ECO:0000250}. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. {ECO:0000250}.
Tissue Specificity Testis-specific.
Top Pathways Protein processing in endoplasmic reticulum, Spliceosome, Toxoplasmosis, Endocytosis, Influenza A

HSP70 Monoclonal Antibody (3A3) - MA3-006 from Thermo Fisher Scientific

Prices $375.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3A3
Immunoprecipitation of Heat Shock Protein 70 (Hsp70) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500ug whole cell lysate with 2ug of Hsp70 monoclonal antibody (Product # MA3-006) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Plus Agarose (Product # 20421), washed extensively, and eluted with Lane Marker Reducing Sample Buffer (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp70 monoclonal antibody (Product # MA3-006) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

HSP70 Monoclonal Antibody (BRM-22) - MA1-91159 from Thermo Fisher Scientific

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone BRM-22
Western blot analysis of HSP70/Heat Shock Protein 70 in Bovine brain extract. Samples were probed with a HSP70/Heat Shock Protein 70 monoclonal antibody (Product # MA1-91159) at a dilution of 1:10,000. The antibody was developed with AlkPhos APA mouse Fab ads HIgG and a NBTBCIP substrate.

HSP70 Monoclonal Antibody (C92F3A-5) - MA1-10889 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone C92F3A-5
Immunoprecipitation of Heat Shock Protein 70 (Hsp70) was performed on THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of an Hsp70 monoclonal antibody (Product # MA1-10889) overnight at 4?C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted with 6X Laemmli non-reducing sample buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an Hsp70 monoclonal antibody (Product # MA1-10889) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.

HSPA1A antibody - orb97670 from Biorbyt

Prices $187.00, $383.00
Sizes 80 μl (Trial size), 400 μl
Host Mouse
Clonality Monoclonal
Clone 201CT8.2.3
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