FANCI

Gene Symbol	FANCI
Entrez Gene	55215
Alt Symbol	KIAA1794
Species	Human
Gene Type	protein-coding
Description	Fanconi anemia, complementation group I
Other Description	Fanconi anemia group I protein
Swissprots	Q96ST0 Q9NVI1 Q9BT96 A6NJZ0 Q96JN1 A4ZVE4 A5YMH4
Accessions	EAX02039 EAX02040 EAX02041 EAX02042 EAX02043 EAX02044 Q9NVI1 AB058697 BAB47423 AK001581 BAA91770 AK027564 BAB55200 AK055176 BAG51480 BC004277 AAH04277 BC021859 BC140769 AAI40770 BC144483 AAI44484 BC146804 BC166621 AAI66621 BM461935 BQ050477 BU187299 CN365698 EF469766 ABP88002 EF567077 ABQ63084 XM_011521756 XP_011520058 XM_011521757 XP_011520059 XM_011521758 XP_011520060 XM_011521759 XP_011520061 XM_011521760 XP_011520062 XM_011521761 XP_011520063 XM_011521762 XP_011520064 XM_011521763 XP_011520065 XM_011521764 XP_011520066 XM_011521765 XP_011520067 XM_011521766 XP_011520068 XM_011521767 XP_011520069 XM_011521768 XP_011520070 XM_011521769 XP_011520071 NM_001113378 NP_001106849 NM_018193 NP_060663
Function	Plays an essential role in the repair of DNA double- strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single- stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage. {ECO:0000269\|PubMed:17412408, ECO:0000269\|PubMed:17452773, ECO:0000269\|PubMed:17460694, ECO:0000269\|PubMed:19111657}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:17412408, ECO:0000269\|PubMed:17460694, ECO:0000269\|PubMed:19465922}. Note=Observed in spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites. They are frequently interlinked through BLM-associated ultra-fine DNA bridges.
Top Pathways	Fanconi anemia pathway

FANCI (A-7) - sc-271316 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

FANCI (A-7): sc-271316. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

FANCI (H-102) - sc-98532 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

FANCI (H-102): sc-98532. Western blot analysis of FANCI expression in non-transfected 293T: sc-117752 (A), mouse FANCI transfected 293T: sc-126833 (B) and HeLa (C) whole cell lysates.

FANCI (S-15) - sc-104234 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

FANCI (S-15): sc-104234. Western blot analysis of FANCI expression in MDA-MB-231 (A) and U-2 OS (B) whole cell lysates and HeLa nuclear extract (C).

Anti-FANCI antibody - ab15344 from Abcam

Prices	$392.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-FANCI antibody (ab15344) at 1/5000 dilutionLane 1 : HeLa cell nuclear extract 5 µg.Lane 2 : HeLa cell nuclear extract 20 µg.Observed band size : 145 kDa (why is the actual band size different from the predicted?)Additional bands at : 160 kDa,230 kDa,70 kDa. We are unsure as to the identity of these extra bands.Exposure time : 15 minutesDetection: chemiluminescence.

Anti-FANCI antibody - ab74332 from Abcam

Prices	$382.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-FANCI antibody (ab74332) at 0.04 µg/mlLane 1 : Whole HeLa cell lysate at 50 µgLane 2 : Whole HeLa cell lysate at 15 µgLane 3 : Whole HeLa cell lysate at 5 µgLane 4 : Whole 293T cell lysate at 50 µg

Anti-FANCI antibody - ab103077 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-FANCI antibody (ab103077) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-FANCI antibody - ab15346 from Abcam

Prices	$376.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Rabbit polyclonal to KIAA1794 (ab15346) at 1/5000.Lane 1: 5µg HeLa cell nuclear extractLane 2: 20 µg HeLa cell nuclear extractDetection: chemiluminescence with an exposure time of 15 minutes.

Anti-FANCI antibody - ab169663 from Abcam

Host	Mouse
Clonality	Polyclonal

All lanes : Anti-FANCI antibody (ab169663) at 1/500 dilutionLane 1 : FANCI-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-FANCI antibody - C-terminal - ab135065 from Abcam

Host	Rabbit
Clonality	Polyclonal

Anti-FANCI antibody - C-terminal (ab135065) at 1/1000 dilution + HeLa cell lysate

Anti-FANCI Antibody (aa1025-1075) - LS-C65899 from LifeSpan Bioscience

Prices	$545.00
Sizes	20 µg
Host	Rabbit
Clonality	Polyclonal

Anti-FANCI Antibody (aa200-250) - LS-C71329 from LifeSpan Bioscience

Prices	$575.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-FANCI Antibody (aa450-500) - LS-C71330 from LifeSpan Bioscience

Prices	$575.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-FANCI Antibody (aa1025-1075) - LS-C288063 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-FANCI Antibody (aa450-500) - LS-C287131 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human KIAA1794/FLJ10719 by Western Blot. Samples: Nuclear extract (5 and 20 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-KIAA1794/FLJ10719 antibody used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.

Anti-FANCI Antibody (aa200-250) - LS-C285986 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

FANCI Antibody - NB100-60447 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: FANCI Antibody [NB100-60447] - Detection of Human FANCI on HeLa whole cell lysate using NB100-60447. FANCI was also immunoprecipitated, albeit less efficiently, by another rabbit anti-FANCI antibody.

FANCI Antibody - NB300-243 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

FANCI Antibody - NBP1-84608 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

FANCI Antibody - NB300-244 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: FANCI Antibody [NB300-244] - Nuclear extract (5 and 20 ug) from HeLa cells. Antibody used at 0.2 ug/ml.

FANCI Antibody - 22630002 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

FANCI Antibody - H00055215-B01 from Novus Biologicals

Host	Mouse
Clonality	Polyclonal

Western Blot: FANCI Antibody [H00055215-B01] - analysis of KIAA1794 expression in transfected 293T cell line by KIAA1794 MaxPab polyclonal antibody (B01). Lane 1: KIAA1794 transfected lysate (8.14 KDa). Lane 2: Non-transfected lysate.

Anti-FANCI antibody produced in rabbit - HPA039972 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-FANCI: <B>Cat. No. HPA039972</B>: Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

FANCI antibody - orb66890 from Biorbyt

Prices	$425.00
Sizes	100 μl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of total cell extracts from human Hela using FANCI antibody

FANCI antibody - orb66888 from Biorbyt

Prices	$425.00
Sizes	100 μl
Host	Rabbit
Clonality	Polyclonal

FANCI antibody - orb66889 from Biorbyt

Prices	$425.00
Sizes	100 μl
Host	Rabbit
Clonality	Polyclonal

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