NAE1

Gene Symbol NAE1
Entrez Gene 8883
Alt Symbol A-116A10.1, APPBP1, HPP1, ula-1
Species Human
Gene Type protein-coding
Description NEDD8 activating enzyme E1 subunit 1
Other Description APP-BP1|NEDD8-activating enzyme E1 regulatory subunit|NEDD8-activating enzyme E1 subunit|amyloid beta precursor protein binding protein 1, 59kDa|amyloid beta precursor protein-binding protein 1, 59 kDa|amyloid beta precursor protein-binding protein 1, 59kD|amyloid protein-binding protein 1|proto-oncogene protein 1|protooncogene protein 1
Swissprots B3KUP9 A6NFN4 Q13564 B2R700 A8MU28 A6NCK0
Accessions AAC23784 CCQ43241 CDM22116 EAW83042 EAW83043 Q13564 AK097680 BAG53511 AK298159 BAH12735 AK307677 AK312784 BAG35647 AL136798 CAB66732 AY197612 AAP35030 BC000480 AAH00480 BC013301 AAH13301 BC041323 BM045535 BM785899 DQ892741 ABM83667 DQ895987 ABM86913 U50939 AAC50477 XM_005256215 XP_005256272 XM_011523422 XP_011521724 XM_011523423 XP_011521725 NM_001018159 NP_001018169 NM_001018160 NP_001018170 NM_001286500 NP_001273429 NM_003905 NP_003896
Function Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:10722740, ECO:0000269|PubMed:12740388}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:14557245}. Note=Colocalizes with APP in lipid rafts.
Tissue Specificity Ubiquitous in fetal tissues. Expressed throughout the adult brain. {ECO:0000269|PubMed:8626687}.
Top Pathways Alzheimer's disease

NAE1/APPBP1 (D9I4Z) Rabbit mAb - 14321 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9I4Z
Western blot analysis of extracts from various cell lines using NAE1/APPBP1 (D9I4Z) Rabbit mAb.

APPBP1 (D-25) - sc-130690 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
APPBP1 (D-25): sc-130690. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing cytoplasmic and membrane localization.

APPBP1 (C-2) - sc-390002 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
APPBP1 (C-2): sc-390002. Western blot analysis of APPBP1 expression in HeLa (A) and T98G (B) whole cell lysates.

APPBP1 (P-20) - sc-79844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
APPBP1 (P-20): sc-79844. Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.

APPBP1 (K-20) - sc-79843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

APPBP1 (H-187) - sc-366048 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
APPBP1 (H-187): sc-366048. Western blot analysis of APPBP1 expression in HeLa (A), T98G (B), A-431 (C) and K-562 (D) whole cell lysates.

APPBP1 (20) - sc-135839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
APPBP1 (20): sc-135839. Western blot analysis of APPBP1 expression in RSV-3T3 whole cell lysate.

Anti-APPBP1 antibody - ab56420 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-APPBP1 antibody [EPR15732] - ab187142 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15732
All lanes : Anti-APPBP1 antibody [EPR15732] (ab187142) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : Human fetal muscle tissue lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-APPBP1 antibody - C-terminal - ab71526 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-APPBP1 antibody - C-terminal (ab71526) at 1/60 dilution + mouse brain tissue lysate at 35 µg

Anti-APPBP1 antibody - C-terminal - ab197847 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-APPBP1 antibody - C-terminal (ab197847) at 1/500 dilutionLane 1 : Human fetal muscle tissue lysateLane 2 : Human fetal kidney tissue lysateLane 3 : Human fetal liver tissue lysateLysates/proteins at 40 µg per lane.

Anti-APPBP1 antibody - C-terminal - ab189791 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-APPBP1 antibody - C-terminal (ab189791) at 1/500 dilutionLane 1 : Mouse brain total cell extractsLane 2 : Mouse thymus total cell extracts

Anti-APPBP1 antibody - Carboxyterminal end - ab71592 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-APPBP1 antibody - Carboxyterminal end (ab71592) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysatesLane 2 : APPBP1 transfected 293 cell lysatesLysates/proteins at 2 µg per lane.

Anti-APPBP1 antibody - N-terminal - ab71542 from Abcam

Prices $304.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-APPBP1 antibody - N-terminal (ab71542) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the APPBP1 geneLysates/proteins at 2 µg per lane.

Anti-NAE1 / APPBP1 Antibody (aa172-200, AP) - LS-C263940 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (aa172-200, APC) - LS-C263941 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (aa172-200, Biotin) - LS-C263942 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (aa172-200, FITC) - LS-C263943 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (aa172-200, Azide-free, HRP) - LS-C263944 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (aa172-200, PE) - LS-C263945 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (C-Terminus, AP) - LS-C275099 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (C-Terminus, Biotin) - LS-C275101 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (C-Terminus, APC) - LS-C275100 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (C-Terminus, Azide-free, HRP) - LS-C275103 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAE1 / APPBP1 Antibody (C-Terminus, PE) - LS-C275104 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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