EYA3

Gene Symbol	EYA3
Entrez Gene	2140
Alt Symbol	-
Species	Human
Gene Type	protein-coding
Description	EYA transcriptional coactivator and phosphatase 3
Other Description	eyes absent 3\|eyes absent homolog 3
Swissprots	B4DIR7 A8K190 Q99813 Q8IVX7 O95463 Q99504 B4DNZ7
Accessions	CAA71311 EAX07713 EAX07714 Q99504 AJ007991 CAA07814 AK289805 BAF82494 AK295745 BAG58579 AK298129 BAG60409 AK303664 BAG64663 AL603098 BC029500 AAH29500 BC041667 AAH41667 BQ018346 BX648945 CA434977 CR748247 DC416048 DQ426872 DQ576790 EU446857 ABZ92386 U81602 AAB42066 XM_006710449 XP_006710512 XM_011540999 XP_011539301 XM_011541000 XP_011539302 XM_011541001 XP_011539303 XM_011541002 XP_011539304 XM_011541003 XP_011539305 XM_011541004 XP_011539306 XM_011541005 XP_011539307 XM_011541006 XP_011539308 XR_946582 NM_001282560 NP_001269489 NM_001282561 NP_001269490 NM_001282562 NP_001269491 NM_001990 NP_001981 NR_104214 NM_172098
Function	Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye. {ECO:0000250, ECO:0000269\|PubMed:19234442, ECO:0000269\|PubMed:19351884}.
Subcellular Location	Cytoplasm {ECO:0000250}. Nucleus {ECO:0000269\|PubMed:19234442}. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively (By similarity). {ECO:0000250}.

EYA3 (N-16) - sc-15101 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

EYA3 (N-16): sc-15101. Western blot analysis of EYA3 expression in Hep G2 whole cell lysate.

EYA3 (G-16) - sc-15103 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-EYA3 antibody - ab22835 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ICC/IF image of ab22835 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22835, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-EYA3 antibody - ab95876 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EYA3 with ab95876 at 1/200 (1µg/ml). Detection: DAB.

Anti-EYA3 antibody - ab178598 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-EYA3 antibody (ab178598) at 1 µg/ml + Human thymus tumour lysate at 10 µg

Anti-EYA3 antibody - ab95877 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-EYA3 antibody - ab129501 from Abcam

Prices	$428.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ab129501, at 1/250 dilution, staining EYA3 in formalin-fixed, paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry using DAB staining.

Anti-EYA3 Antibody (aa30-60) - LS-C289413 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-EYA3 Antibody (aa237-286) - LS-C187218 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Human Thymus Tumor. EYA3 antibody dilution 1.0 ug/ml.

Anti-EYA3 Antibody (aa277-326) - LS-C29714 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-EYA3 Antibody (N-Terminus) - LS-C201253 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Human Fetal Spleen. EYA3 antibody dilution 1.0 ug/ml.

Anti-EYA3 Antibody (aa1-30) - LS-C286942 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human EYA3 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-EYA3 used at a dilution of 1:250.

Anti-EYA3 Antibody (aa1-30) - LS-C289412 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human EYA3 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA3 used at a dilution of 1:200 (1 Detection: DAB.

EYA3 Antibody - NBP1-46818 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: EYA3 Antibody [NBP1-46818] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody NBP1-46818 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody NBP1-46819, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

EYA3 Antibody - NBP1-46819 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunoprecipitation: EYA3 Antibody [NBP1-46819] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody NBP1-46819 used for IP at 3 ug/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody NBP1-46818, which recognizes an upstream epitope. For blotting immunoprecipitated EYA3, NBP1-46818 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

EYA3 Antibody - NBP2-04075 from Novus Biologicals

Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody - bs-12382R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-12382R-A350 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-12382R-A488 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-12382R-A555 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-12382R-A647 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody, Cy3 Conjugated - bs-12382R-Cy3 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody, Cy5 Conjugated - bs-12382R-Cy5 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody, Cy5.5 Conjugated - bs-12382R-Cy5.5 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

EYA3 Polyclonal Antibody, Cy7 Conjugated - bs-12382R-Cy7 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

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