RAMP1

Gene Symbol	RAMP1
Entrez Gene	10267
Alt Symbol	-
Species	Human
Gene Type	protein-coding
Description	receptor (G protein-coupled) activity modifying protein 1
Other Description	CRLR activity-modifying protein 1\|calcitonin receptor-like receptor activity modifying protein 1\|calcitonin-receptor-like receptor activity-modifying protein 1\|receptor (calcitonin) activity modifying protein 1\|receptor activity modifying protein 1\|receptor activity-modifying protein 1
Swissprots	O60894 Q6FGS5
Accessions	AAY14674 AAY24219 EAW71127 O60894 AI017108 AJ001014 CAA04472 AY265457 AAP23298 BC000548 AAH00548 BI548986 BM126103 BM978161 BX327017 CR542032 CAG46829 CR542044 CAG46841 DQ891905 ABM82831 DQ895091 ABM86017 XM_011510477 XP_011508779 XM_011510478 XP_011508780 NM_001308353 NP_001295282 NM_005855 NP_005846
Function	Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. {ECO:0000269\|PubMed:9620797}.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Tissue Specificity	Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract. {ECO:0000269\|PubMed:9620797}.
Top Pathways	Vascular smooth muscle contraction

RAMP1 (FL-148) - sc-11379 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

RAMP1 (FL-148): sc-11379. Western blot analysis of RAMP1 expression in PC-3 (A), ES-2 (B) and MCF7 (C) whole cell lysates and mouse heart tissue extract (D).

RAMP1 (N-20) - sc-8850 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

RAMP1 (C-20) - sc-8851 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

RAMP1 (C-20): sc-8851. Western blot analysis of RAMP1 expression in human heart tissue extract.

Anti-RAMP1 antibody - ab194787 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RAMP1 antibody (ab194787) at 1/500 dilution + HepG2 cell extract

Anti-RAMP1 antibody - ab96125 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RAMP1 antibody (ab96125) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-RAMP1 antibody - ab135943 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RAMP1 antibody (ab135943) at 1/500 dilution + MCF7 cell extracts at 30 µg

Anti-RAMP1 antibody [EPR10867] - ab156575 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR10867

Overlay histogram showing Caco-2 cells stained with ab156575 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab156575, 1/1000 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Caco-2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-RAMP1 Antibody (C-Terminus) - LS-C9909 from LifeSpan Bioscience

Prices	$415.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RAMP1 Antibody - LS-C144133 from LifeSpan Bioscience

Prices	$605.00
Sizes	100 µg
Host	Sheep
Clonality	Polyclonal

Anti-RAMP1 Antibody (aa98-113) - LS-C148888 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Anti-RAMP1 Antibody (aa76-84) - LS-C87301 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Antibody (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-RAMP1 Antibody (aa23-72) - LS-C199984 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from MCF7 cells, using RAMP1 antibody.

Anti-RAMP1 Antibody - LS-C334726 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using RAMP1 antibody.

Anti-RAMP1 Antibody (aa84-148) - LS-C185999 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. RAMP1 antibody diluted at 1:1000.

RAMP1 Antibody [Unconjugated] - AF6428 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep
Clonality	Polyclonal

Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RAMP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

RAMP1 Antibody - H00010267-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

RAMP1 Antibody (1F1) - H00010267-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F1

Sandwich ELISA: RAMP1 Antibody (1F1) [H00010267-M01] - Detection limit for recombinant GST tagged RAMP1 is approximately 1ng/ml as a capture antibody.

RAMP1 Antibody (3B9) - H00010267-M04 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3B9

Sandwich ELISA: RAMP1 Antibody (3B9) [H00010267-M04] - Detection limit for recombinant GST tagged RAMP1 is 0.3 ng/ml as a capture antibody.

Anti-RAMP1 antibody produced in rabbit - SAB2701014 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-RAMP1: <B>Cat. No. SAB2701014</B>: Western Blot analysis of RAMP1 in extracts from <B>Lanes</B><BR/>A. HepG2 using <B>Cat. No. SAB2701014</B> at a 1:1000 dilution.

Monoclonal Anti-RAMP1 antibody produced in mouse - WH0010267M1 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F1

<B>Sandwich ELISA</B><BR/>Monoclonal Anti-RAMP1, <B>Cat. No. WH0010267M1</B>: the detection limit for recombinant GST tagged RAMP1 is approximately 1ng/mL.

RAMP1-Antibody-monoclonal-M01 - AT3558a from Abgent, a WuXi AppTec company

Prices	$315.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F1

Detection limit for recombinant GST tagged RAMP1 is approximately 1ng/ml as a capture antibody.

Ramp1-C-Term-mouse-Antibody - AF1905a from Abgent, a WuXi AppTec company

Prices	$335.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AF1905a (0.3 µg/ml) staining of Rat Brain lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Human RAMP1 Antibody - AF6428 from R&D Systems

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep

Detection of Human RAMP1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RAMP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for RAMP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human RAMP1 Affinity Purified Polyclonal Ab, Sheep IgG - AF6428-SP from R&D Systems

Host	Sheep
Clonality	Polyclonal

RAMP1 Antibody (OAAF05936) - OAAF05936 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

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