Gene Symbol | DAD1 |
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Entrez Gene | 1603 |
Alt Symbol | OST2 |
Species | Human |
Gene Type | protein-coding |
Description | defender against cell death 1 |
Other Description | DAD-1|dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1|oligosaccharyl transferase subunit DAD1|oligosaccharyltransferase 2 homolog|oligosaccharyltransferase subunit 2 (non-catalytic) |
Swissprots | P61803 D3DS25 Q6FGA3 |
Accessions | AAB58540 AAO74827 EAW66252 EAW66253 P61803 AK223129 BAD96849 BC007403 AAH07403 BC009798 AAH09798 BC038575 BP198316 CR407682 CAG28610 CR542204 CAG47001 D15057 BAA03650 DQ892868 ABM83794 DQ896117 ABM87116 NM_001344 NP_001335 |
Function | Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis (By similarity). {ECO:0000250}. |
Subcellular Location | Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. |
Top Pathways | N-Glycan biosynthesis, Protein processing in endoplasmic reticulum |
DAD1 CRISPR/Cas9 KO Plasmid (h) - sc-404172 from Santa Cruz Biotechnology
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DAD1 HDR Plasmid (h) - sc-404172-HDR from Santa Cruz Biotechnology
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DAD1 Double Nickase Plasmid (h) - sc-404172-NIC from Santa Cruz Biotechnology
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DAD1 Double Nickase Plasmid (h2) - sc-404172-NIC-2 from Santa Cruz Biotechnology
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DAD1 CRISPR Activation Plasmid (h) - sc-404172-ACT from Santa Cruz Biotechnology
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DAD1 CRISPR Activation Plasmid (h2) - sc-404172-ACT-2 from Santa Cruz Biotechnology
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DAD1 Lentiviral Activation Particles (h) - sc-404172-LAC from Santa Cruz Biotechnology
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DAD1 Lentiviral Activation Particles (h2) - sc-404172-LAC-2 from Santa Cruz Biotechnology
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DAD1 siRNA (h) - sc-40786 from Santa Cruz Biotechnology
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DAD1 shRNA Plasmid (h) - sc-40786-SH from Santa Cruz Biotechnology
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DAD1 shRNA (h) Lentiviral Particles - sc-40786-V from Santa Cruz Biotechnology
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DAD1 RNAi - H00001603-R01 from Novus Biologicals
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MISSION® esiRNA - EHU001291 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN6917 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND6917 from Sigma-Aldrich
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DAD1 siRNA (Human) - CRH1126 from Cohesion Biosciences
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DAD1 - MBS8237084 from MyBioSource
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shRNA set against Human DAD1 (NM_001344.2) - SHH274409 from Creative biogene
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shRNA set against Human DAD1(NM_001344.2) - SHG221041 from Creative biogene
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DAD1 shRNA Plasmid (Human) - CHH1126 from Cohesion Biosciences
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DAD1 miRNA 3'UTR clone - MiUTR1H-02658 from Creative biogene
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