ab10559 rabbit polyclonal to CD45 (1/500) with secondary goat anti-rabbit IgG ab6721 (1/5000). Expected molecular weight: 147 kDa. WB exposure time: 1min 30sec Lanes 1 to 10: 20 µ g of cell lysate per lane Lanes 6 to 10: ab17550 CD45 blocking peptide used at 1 µ g/ml Lane 1: HeLa Nuclear Extract ( ab10559 ) Lane 2: HeLa Whole Cell Lysate ( ab10559 ) Lane 3: A431 Whole Cell Lysate ( ab10559 ) Lane 4: Jurkat Whole Cell Lysate ( ab10559 ) Lane 5: HEK293 Whole Cell Lysate ( ab10559 ) Lane 6: HeLa Nuclear Extract ( ab10559 + ab17550) Lane 7: HeLa Whole Cell Lysate ( ab10559 + ab17550) Lane 8: A431 Whole Cell Lysate ( ab10559 + ab17550) Lane 9: Jurkat Whole Cell Lysate ( ab10559 + ab17550) Lane 10: HEK293 Whole Cell Lysate ( ab10559 + ab17550) Binding of