LIPI

Gene Symbol LIPI
Entrez Gene 149998
Alt Symbol CT17, LPDL, PLA1C, PRED5, mPA-PLA1 beta
Species Human
Gene Type protein-coding
Description lipase, member I
Other Description LPD lipase|cancer/testis antigen 17|lipase member I|membrane-associated phosphatidic acid-selective phospholipase A1-beta
Swissprots Q6XZB0 G1JSG4 G1JSG6 G1JSG8 G1JSG7 G1JSG9 G1JSG3 G1JSG5
Accessions Q6XZB0 AI017218 AY197607 AAP37476 AY918476 AAX14040 BC140336 AAI40337 BC148691 AAI48692 BI460152 JN387910 AEL12688 JN387911 AEL12689 JN387912 AEL12690 JN387913 AEL12691 JN387914 AEL12692 JN387915 AEL12693 JN387916 AEL12694 XM_006723965 XP_006724028 XM_006723966 XP_006724029 NM_001302998 NP_001289927 NM_001302999 NP_001289928 NM_001303000 NP_001289929 NM_001303001 NP_001289930 NM_198996 NP_945347 NM_145317
Function Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG). {ECO:0000269|PubMed:12963729}.
Subcellular Location Isoform 1: Membrane. Secreted. Note=May associate with lipid draft.
Tissue Specificity Expressed in testis. Expressed exclusively at the connecting piece of the sperm. {ECO:0000269|PubMed:12719377, ECO:0000269|PubMed:12963729}.

LPD lipase (T-17) - sc-54087 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-LIPI antibody - ab202348 from Abcam

Prices $359.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-LIPI antibody (ab202348) at 1/1000 dilution + Human testis tissue lysate at 20 µgSecondarygoat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

Anti-LIPI antibody - ab106123 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-LIPI antibody (ab106123) at 1 µg/ml + NCI-H226 cell lysate at 10 µg

Anti-LIPI antibody - ab168208 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-LIPI antibody (ab168208) at 1 µg/mlLane 1 : Lysate from 293T cells transfected with LIPILane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-LIPI Antibody - LS-C295156 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-LIPI Antibody (aa272-321) - LS-C292140 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-LIPI Antibody (FITC) - LS-C303127 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-LIPI Antibody (Biotin) - LS-C305826 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-LIPI Antibody (aa289-338) - LS-C120064 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from COLO cells, using LIPI Antibody. The lane on the right is treated with the synthesized peptide.

Anti-LIPI Antibody (aa359-408) - LS-C111075 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
LIPI antibody LS-C111075 Western blot of NCI-H226 cell lysate.

Anti-LIPI Antibody (aa224-258) - LS-C344467 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysate from human testis tissue lysate with LIPI Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Lipase I Antibody - AF5909 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal

Lipase I Antibody - NBP1-56620 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LIPI Antibody [NBP1-56620] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.

Lipase I Antibody - H00149998-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot analysis of LIPI expression in transfected 293T cell line by LIPI MaxPab polyclonal antibody. Lane 1: LIPI transfected lysate (52.91 KDa). Lane 2: Non-transfected lysate.

Lipase I Antibody - NBP1-85412 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: Lipase I Antibody [NBP1-85412] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Lipase I Antibody - H00149998-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-LIPI antibody produced in rabbit - SAB2105657 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-LIPI: <B>Cat. No. SAB2105657</B>: LIPI in NCI-H226 cell lysates with LIPI antibody at 1.0 μg/mL.

Anti-LIPI antibody produced in rabbit - HPA029950 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-LIPI: <B>Cat. No. HPA029950</B>: Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-LIPI antibody produced in rabbit - SAB4500547 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-LIPI: <B>Cat. No. SAB4500547</B>: Immunoblotting analysis of extracts from COLO cells, using Anti-LIPI antibody. The lane on the left was treated with the Anti-LIPI antibody. The lane on the right (negative control) was treated with both Anti-LIPI antibody and the synthesized immunogen peptide.

LIPI antibody - middle region (ARP45940_P050) - ARP45940_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

LIPI-Antibody-Center - AP20877a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysate from human testis tissue lysate, using LIPI Antibody (Center)(Cat. #AP20877a). AP20877a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

LIPI-Antibody-Center - AP21120a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysate from human testis tissue lysate, using LIPI Antibody (Center)(Cat. #AP21120a). AP21120a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

LIPI antibody - GTX87435 from GeneTex

Prices $319.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Anti-LIPI antibody used in Western blot. GTX87435

Human Lipase I Antibody - AF5909 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Detection of Human Lipase I by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019 ). A specific band was detected for Lipase I at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .

Human Lipase I Affinity Purified Polyclonal Ab, Goat IgG - AF5909-SP from R&D Systems

Host Goat
Clonality Polyclonal
Detection of Human Lipase I by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipase I at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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