GFI1B

Gene Symbol GFI1B
Entrez Gene 8328
Alt Symbol BDPLT17
Species Human
Gene Type protein-coding
Description growth factor independent 1B transcription repressor
Other Description growth factor independent 1B (potential regulator of CDKN1A, translocated in CML)|potential regulator of CDKN1A translocated in CML|zinc finger protein Gfi-1b
Swissprots Q6T888 O95270 Q5VTD9 Q5VTD8 Q6FHZ2
Accessions CDL93510 EAW88023 EAW88024 EAW88025 Q5VTD9 AF081946 AAD08672 AI097490 AY428733 AAR06639 BC035626 BC043371 AAH43371 BC048254 BX113435 CR536546 CAG38783 XM_006717297 XP_006717360 XM_006717298 XP_006717361 XM_011519068 XP_011517370 XM_011519069 XP_011517371 XM_011519070 XP_011517372 XM_011519071 XP_011517373 XR_929847 NM_001135031 NP_001128503 NM_004188 NP_004179
Function Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down- regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcrip
Subcellular Location Nucleus {ECO:0000269|PubMed:16688220}.
Tissue Specificity Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA). {ECO:0000269|PubMed:12351384, ECO:0000269|PubMed:17156408, ECO:0000269|PubMed:9878267}.

GFI1b (D3G2) Rabbit mAb - 5849 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3G2
Western blot analysis of extracts from various cell lines using GFI1b (D3G2) Rabbit mAb.

Gfi-1B (B-7) - sc-28356 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gfi-1β (B-7): sc-28356. Western blot analysis of Gfi-1β expression in RBL-1 whole cell lysate.

Gfi-1B (S-18) - sc-54811 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Gfi-1B (S-18): sc-54811. Western blot analysis of Gfi-1B expression in Jurkat (A), K-562 (B) and HEL 92.1.7 (C) nuclear extracts.

Gfi-1B (H-150) - sc-22795 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Gfi-1B (H-150): sc-22795. Western blot analysis of Gfi-1B expression in K-562 (A), HEL 92.1.7 (B), Jurkat (C), LADMAC (D) and Ramos (E) nuclear extracts.

Gfi-1B (P-20) - sc-54810 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-GFI1B antibody - ab26132 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GFI1B antibody (ab26132) at 1 µg/ml + Jurkat cell lysateSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-GFI1B antibody - ab119379 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GFI1B antibody (ab119379) at 1/500 dilution + Raji cell extract

Anti-GFI1B antibody - ab200744 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GFI1B antibody (ab200744)Lane 1 : HEK293T whole cell lysateLane 2 : sp2/0 whole cell lysateLane 3 : H9C2 whole cell lysate

Anti-GFI1B antibody - ab167304 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-GFI1B antibody (ab167304) at 1 µg/mlLane 1 : GFI1B transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-GFI1B Antibody (C-Terminus, clone D3G2) - LS-C183867 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone D3G2

Anti-GFI1B Antibody (aa322-326) - LS-C205231 from LifeSpan Bioscience

Prices $265.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Raji cells using GFI1b antibody.

Anti-GFI1B Antibody (aa302-330) - LS-C203993 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
GFI1B Antibody western blot of HL-60,K562,U266 cell line and mouse spleen lysates (35 ug/lane). The GFI1B antibody detected the GFI1B protein (arrow).

Anti-GFI1B Antibody (N-Terminus) - LS-C29813 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
GFI1B antibody LS-C29813 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

GFI1B Antibody - NBP1-86499 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: GFI1B Antibody [NBP1-86499] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

GFI1B Antibody - H00008328-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: GFI1B Antibody [H00008328-B01] - analysis of GFI1B expression in transfected 293T cell line by GFI1B MaxPab polyclonal antibody (B01). Lane 1: GFI1B transfected lysate (37.50 KDa). Lane 2: Non-transfected lysate.

GFI1B Antibody - H00008328-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

GFI1B Antibody - H00008328-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: GFI1B Antibody [H00008328-B01P] - Analysis of GFI1B expression in transfected 293T cell line by GFI1B polyclonal antibody. Lane 1: GFI1B transfected lysate(36.3 KDa). Lane 2: Non-transfected lysate.

GFI1B Polyclonal Antibody - bs-9881R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GFI1B antibody produced in mouse - SAB1409863 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-GFI1B: <B>Cat. No. SAB1409863</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~36.3 kDa using GFI1B transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-GFI1B antibody produced in rabbit - HPA007012 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-GFI1B: <B>Cat. No. HPA007012</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human stomach tissue showing strong cytoplasmic staining of chief cells.

GFI1B antibody - N-terminal region (ARP30093_P050) - ARP30093_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GFI1B-Antibody-C-term - AP20437b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
GFI1B Antibody (C-term) (Cat. #AP20437b) western blot analysis in HL-60,K562,U266 cell line and mouse spleen lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).

GFI1b antibody - GTX50844 from GeneTex

Prices $319.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal
Anti-GFI1b antibody used in Western blot. GTX50844

GFI1B Antibody - C-terminal region (OAAB17962) - OAAB17962 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

GFI1b antibody - orb43502 from Biorbyt

Prices $417.00, $536.00
Sizes 50 μg, 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of GFI1b antibody in Raji cells lysates
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