POLR2C

Gene Symbol POLR2C
Entrez Gene 5432
Alt Symbol RPB3, RPB31, hRPB33, hsRPB3
Species Human
Gene Type protein-coding
Description polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
Other Description DNA-directed RNA polymerase II 33 kDa polypeptide|DNA-directed RNA polymerase II subunit C|DNA-directed RNA polymerase II subunit RPB3|RNA polymerase II subunit 3|RNA polymerase II subunit B3|RPB33
Swissprots O15161 P19387
Accessions AAC24309 EAW82926 EAW82927 EAW82929 EAW82930 P19387 AF008443 AAC14355 AJ224143 CAA11842 AJ224144 CAA11843 AK293112 BAF85801 AK295582 BAH12113 AK300124 BAH13217 BC000409 AAH00409 BC003159 AAH03159 BC028157 AAH28157 BI458170 CR542041 CAG46838 DQ891411 ABM82337 DQ894586 ABM85512 EU176305 ABW03756 J05448 AAA36586 XM_011523111 XP_011521413 NM_032940 NP_116558 NM_002694
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000269|PubMed:9852112}.
Top Pathways Huntington's disease, Epstein-Barr virus infection, Purine metabolism, RNA polymerase, Pyrimidine metabolism

Anti-POLR2C antibody - ab186867 from Abcam

Prices $382.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling POLR2C with ab186867 at 1/100. Magnification: 400x.

Anti-POLR2C antibody - ab14252 from Abcam

Prices $0.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal

Anti-POLR2C antibody - ab127037 from Abcam

Prices $359.00, $376.00
Sizes 50 µg, 50 µl
Host Rabbit
Clonality Polyclonal
Anti-POLR2C antibody (ab127037) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Anti-POLR2C antibody - ab135210 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-POLR2C antibody (ab135210) at 1/250 dilutionLane 1 : RT-4 lysateLane 2 : U-251 MG lysateLane 3 : Human Plasma lysateLane 4 : Liver lysateLane 5 : Tonsil lysate

Anti-POLR2C antibody [EPR13294(B)] - ab182150 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13294(B)
All lanes : Anti-POLR2C antibody [EPR13294(B)] (ab182150) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : Human fetal kidney lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-POLR2C antibody [EPR13300(B)] - C-terminal - ab181193 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13300(B)
All lanes : Anti-POLR2C antibody [EPR13300(B)] - C-terminal (ab181193) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : Human fetal kidney tissue lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-POLR2C Antibody - LS-C341346 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-POLR2C Antibody (aa186-235) - LS-C187210 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
POLR2C antibody Western Blot of Mouse Testis. Antibody dilution: 1 ug/ml.

Anti-POLR2C Antibody (clone 1C11) - LS-C197943 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C11
Detection limit for recombinant GST tagged POLR2C is 0.3 ng/ml as a capture antibody.

Anti-POLR2C Antibody (aa225-275) - LS-C290426 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RPB3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RPB3 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RPB3 was also immunoprecipitated by rabbit anti-RPB3 antibody BL13244, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Anti-POLR2C Antibody (aa38-238) - LS-C179115 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR2C Antibody (aa200-228) - LS-C344181 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Fluorescent image of HeLa cells stained with POLR2C Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Anti-POLR2C Antibody - LS-C331689 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded mouse lung using POLR2C antibody at dilution of 1:100 (400x lens).

POLR2C Antibody - NBP1-80817 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: POLR2C Antibody [NBP1-80817] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

POLR2C Antibody - NBP1-80818 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: POLR2C Antibody [NBP1-80818] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

POLR2C Antibody - NBP2-19885 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

POLR2C Antibody - NBP2-32319 from Novus Biologicals

Prices $299.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

POLR2C Antibody (1C11) - H00005432-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C11
Sandwich ELISA: POLR2C Antibody (1C11) [H00005432-M01] - Detection limit for recombinant GST tagged POLR2C is 0.3 ng/ml as a capture antibody.

Anti-POLR2C antibody produced in rabbit - HPA040919 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-POLR2C: <B>Cat. No. HPA040919</B>: Immunohistochemical staining of human colon shows moderate nuclear and cytoplasmic positivity in glandular cells.

Monoclonal Anti-POLR2C antibody produced in mouse - SAB1401292 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C11

Anti-POLR2C (211-225) antibody produced in rabbit - SAB1100540 from Sigma-Aldrich

Prices $309.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-POLR2C (211-225) (RB): <B>Cat. No. SAB1100540</B>: Immunofluorescence of HUVEC cells using Anti-POLR2C (211-225) (RB) at a 1:200 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

POLR2C Antibody - C-terminal region (ARP57690_P050) - ARP57690_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

POLR2C-Antibody-C-term - AP20520b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Fluorescent image of Hela cells stained with POLR2C Antibody (C-term)(Cat#AP20520B). AP20520B was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

POLR2C Antibody - C-terminal region (OAAB18043) - OAAB18043 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

POLR2C antibody - orb78448 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin embedded cervical carcinoma showing nuclear staining using POLR2C antibody
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