UBA2

Gene Symbol UBA2
Entrez Gene 10054
Alt Symbol ARX, HRIHFB2115, SAE2
Species Human
Gene Type protein-coding
Description ubiquitin-like modifier activating enzyme 2
Other Description SUMO-1 activating enzyme subunit 2|SUMO-activating enzyme subunit 2|SUMO1 activating enzyme subunit 2|UBA2, ubiquitin-activating enzyme E1 homolog|anthracycline-associated resistance ARX|ubiquitin-like 1-activating enzyme E1B|ubiquitin-like modifier-activating enzyme 2
Swissprots O95605 Q6IBP6 Q59H87 Q9UED2 Q9NTJ1 B3KWB9 Q9UBT2
Accessions Q9UBT2 AB015337 BAA34795 AB208872 BAD92109 AF079566 AAD23914 AF090384 AAD12784 AF110957 AAD24434 AK023120 BAG51158 AK024114 BAG51265 AK124730 BAG54081 AK299514 BAH13056 AK315521 BAG37902 AL136905 CAB66839 BC003153 AAH03153 BG503066 BG678201 BM556031 BT009781 AAP88783 CR456756 CAG33037 DQ893423 ABM84349 EU176757 ABW03558 U35832 AAC99992 XM_005258404 XP_005258461 XM_006722962 XP_006723025 XM_011526304 XP_011524606 XR_935712 NM_005499 NP_005490
Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. {ECO:0000269|PubMed:11451954, ECO:0000269|PubMed:11481243, ECO:0000269|PubMed:15660128, ECO:0000269|PubMed:17643372, ECO:0000269|PubMed:19443651, ECO:0000269|PubMed:20164921}.
Subcellular Location Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention.
Top Pathways Ubiquitin mediated proteolysis

UBA2 (D15C11) Rabbit mAb - 8688 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D15C11
Western blot analysis of extracts from various cell lines using UBA2 (D15C11) Rabbit mAb.

UBA2 Antibody - 5293 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using UBA2 Antibody.

UBA2 (B-6) - sc-376305 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
UBA2 (B-6): sc-376305. Western blot analysis of UBA2 expression in K-562 whole cell lysate.

UBA2 (C-20) - sc-47540 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
UBA2 (C-20): sc-47540. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts and Leydig cells.

UBA2 (H-155) - sc-135248 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
UBA2 (H-155): sc-135248. Western blot analysis of UBA2 expression in K-562 (A) and HeLa (B) whole cell lysates.

UBA2 (N-16) - sc-47542 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

UBA2 (28) - sc-136359 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
UBA2 (28): sc-136359. Western blot analysis of UBA2 expression in HeLa whole cell lysate.

Anti-SAE2 / UBA2 antibody - ab58451 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SAE2 / UBA2 antibody (ab58451) at 1/500 dilutionLane 1 : extracts from 293 cells treated with UV (5mins)Lane 2 : extracts from 293 cells treated with UV (5mins) with immunizing peptide

Anti-SAE2 / UBA2 antibody - ab95835 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SAE2 / UBA2 antibody (ab95835) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 6 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 7 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SAE2 / UBA2 antibody - ab22104 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SAE2 / UBA2 antibody (ab22104) at 1 µg/ml + HeLa cell lysateObserved band size : 71 kDa (why is the actual band size different from the predicted?)Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

Anti-SAE2 / UBA2 antibody [4A3] - ab118404 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A3
All lanes : Anti-SAE2 / UBA2 antibody [4A3] (ab118404) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLA cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.

Anti-SAE2 / UBA2 antibody [EPR14880] - ab185955 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14880
All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-SAE2 / UBA2 antibody - C-terminal - ab135754 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SAE2 / UBA2 antibody - C-terminal (ab135754) at 1/100 dilution + Jurkat cell lysate at 12.5 µg

Anti-UBA2 antibody - ab177093 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-UBA2 antibody (ab177093) at 1/500 dilutionLane 1 : HeLa cell lysatesLane 2 : HepG2 cell lysatesLane 3 : A549 cell lysates

Anti-UBA2 antibody - C-terminal - ab189289 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-UBA2 antibody - C-terminal (ab189289) at 1/500 dilutionLane 1 : 293 cell extracts, treated with UV 5'Lane 2 : 293 cell extracts, treated with UV 5' with synthesized peptide

Anti-SAE2 / UBA2 Antibody (aa2-14) - LS-C55428 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (aa286-303) - LS-C147933 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (C-Terminus, AP) - LS-C246056 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (C-Terminus, Biotin) - LS-C246058 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (C-Terminus, APC) - LS-C246057 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (C-Terminus, FITC) - LS-C246059 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (C-Terminus, PE) - LS-C246064 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (C-Terminus, Azide-free, HRP) - LS-C247907 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (C-Terminus) - LS-C328801 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SAE2 / UBA2 Antibody (aa590-640) - LS-C289634 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse SAE2 by Immunohistochemistry. Sample: FFPE sections of human prostate carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-SAE2 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
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