MED27

Gene Symbol MED27
Entrez Gene 9442
Alt Symbol CRAP34, CRSP34, CRSP8, TRAP37
Species Human
Gene Type protein-coding
Description mediator complex subunit 27
Other Description CRSP complex subunit 8|cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa|mediator of RNA polymerase II transcription subunit 27|p37 TRAP/SMCC/PC2 subunit|transcriptional coactivator CRSP34
Swissprots Q5VTA5 Q9BU57 V9GYV9 Q4F964 O95401 Q9NYR4 Q6P2C8 Q5VTA4
Accessions EAW87989 EAW87990 EAW87991 EAW87992 EAW87993 Q6P2C8 AF104252 AAD12721 AF230382 AAF37290 AI033804 AK022289 AK124232 AK298436 BAG60657 AK316345 BAH14716 BC002878 AAH02878 BC064608 AAH64608 BC070331 BP255072 BU069864 CD388680 DQ099387 AAZ13763 EU446747 ABZ92276 HG518166 CDI06889 Z36848 XM_005272236 XP_005272293 NM_001253881 NP_001240810 NM_001253882 NP_001240811 NM_004269 NP_004260
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:10882111, ECO:0000269|PubMed:9989412}.
Subcellular Location Nucleus {ECO:0000269|PubMed:10882111, ECO:0000269|PubMed:9989412}.

CRSP34 (A-2) - sc-390295 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CRSP34 (A-2): sc-390295. Western blot analysis of CRSP34 expression in HeLa (A) and K-562 (B) nuclear extracts.

CRSP34 (B-7) - sc-390296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CRSP34 (B-7): sc-390296. Western blot analysis of CRSP34 expression in HeLa (A) and K-562 (B) nuclear extracts.

CRSP34 (C-15) - sc-9423 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CRSP34 (FL-311) - sc-292610 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CRSP34 (FL-311): sc-292610. Western blot analysis of CRSP34 expression in HeLa (A), K-562 (B) and Hep G2 (C) nuclear extracts.

CRSP34 (N-19) - sc-9421 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CRSP34 (40.4) - sc-101187 from Santa Cruz Biotechnology

Prices $295.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
CRSP34 (40.4): sc-101187. Western blot analysis of CRSP34 expression in HeLa nuclear extract.

Anti-CRSP8 antibody - ab199642 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CRSP8 antibody (ab199642) at 1/1000 dilution + HeLa whole cell lysate at 35 µgSecondaryGoat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

Anti-CRSP8 antibody - ab82374 from Abcam

Prices $359.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CRSP8 antibody (ab82374) at 1/50 dilution + K562 cell line lysates at 35 µg

Anti-CRSP8 antibody - ab56539 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-CRSP8 antibody - C-terminal - ab199907 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-CRSP8 antibody - C-terminal (ab199907) at 1000 cellsLane 1 : mouse thymus total cell extractsLane 2 : HeLa total cell extractsLane 3 : Jurkat total cell extracts

Anti-MED27 Antibody (aa1-71, clone 10B2605, Azide-free) - LS-C183524 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10B2605

Anti-MED27 / CRSP8 Antibody - LS-C307097 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MED27 / CRSP8 Antibody - LS-C309998 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MED27 / CRSP8 Antibody (C-Terminus) - LS-C30749 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
A: Marker, B: HepG2 Cell Lysate

Anti-MED27 / CRSP8 Antibody (aa180-229) - LS-C109825 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MED27 / CRSP8 antibody LS-C109825 Western blot of Fetal Brain lysate.

Anti-MED27 / CRSP8 Antibody (clone 8B8) - LS-C133589 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 8B8
Western blot of CRSP8 expression in transfected 293T cell line by CRSP8 monoclonal antibody (M01), clone 8B8.

MED27 Antibody - NBP1-84377 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: MED27 Antibody [NBP1-84377] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

MED27 Antibody (8B8) - H00009442-M01 from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 8B8
Western Blot: MED27 Antibody (8B8) [H00009442-M01] - CRSP8 monoclonal antibody (M01), clone 8B8 Analysis of CRSP8 expression in Hela S3 NE.

MED27 Antibody - H00009442-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: MED27 Antibody [H00009442-D01P] - Analysis of MED27 expression in human kidney.

MED27 Antibody - H00009442-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: MED27 Antibody [H00009442-B01] - analysis of CRSP8 expression in transfected 293T cell line by CRSP8 MaxPab polyclonal antibody (B01). Lane 1: CRSP8 transfected lysate (35.40 KDa). Lane 2: Non-transfected lysate.

MED27 Antibody - H00009442-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-MED27 antibody produced in rabbit - SAB2101457 from Sigma-Aldrich

Prices $347.50
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MED27: <B>Cat. No.SAB2101457</B>: MED27 in Fetal Brain tissue lysate with MED27 antibody at 1.0 μg/mL.

Anti-MED27 antibody produced in rabbit - HPA007002 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CRSP8: <B>Cat. No. HPA007002</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing nuclear as well as cytoplasmic and/or membranous staining of cells in ductus seminiferus.

Anti-MED27 antibody produced in rabbit - SAB1411657 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MED27: <B>Cat. No. SAB1411657</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~35.4 kDa using human kidney lysate.

Anti-MED27 antibody produced in mouse - SAB1406824 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-MED27: <B>Cat. No. SAB1406824</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~34.2 kDa using MED27 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.
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