NISCH

Gene Symbol NISCH
Entrez Gene 11188
Alt Symbol I-1, IR1, IRAS, hIRAS
Species Human
Gene Type protein-coding
Description nischarin
Other Description I-1 receptor candidate protein|I1R candidate protein|imidazoline receptor 1|imidazoline receptor antisera selected
Swissprots Q9Y2I1 Q7Z2X6 C9J245 Q9UFW3 Q9UEU4 Q6PIB4 Q6PGP3 Q9UES6 Q7L8M3
Accessions EAW65228 EAW65229 EAW65230 EAW65231 Q9Y2I1 AB023192 BAA76819 AF058290 AAC33321 AF082516 AAC33104 AI889216 AK001505 AK027331 BAG51306 AK027428 BAG51321 AK075000 AK097732 AK291398 BAF84087 AK297657 BAG60023 AK302441 BAG63739 AK315001 AL117432 CAB55920 AL137405 AL703940 BC038102 AAH38102 BC050552 BC054494 AAH54494 BC056900 AAH56900 BU177208 DB496030 XM_005264839 XP_005264896 XM_006712955 XP_006713018 XM_011533317 XP_011531619 XM_011533318 XP_011531620 NM_001276293 NP_001263222 NM_001276294 NP_001263223 NM_007184 NP_009115
Function Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways (By similarity). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 pro
Subcellular Location Cell membrane. Cytoplasm. Early endosome. Recycling endosome. Note=Enriched in the early/sorting and recycling endosomes. Colocalized in early/sorting endosomes with EEA1 and SNX2 and in recycling endosomes with transferrin receptor. Detected in the perinuclear region partially associated with punctate structures (By similarity). Colocalizes with PAK1 in cytoplasm, vesicular structures in the perinuclear area and membrane ruffles (By similarity). Colocalizes with RAC1 in the cytoplasm and vesicles structures (By similarity). Colocalized with MAPK1 and MAPK3 in RVLM neurons (By similarity). {ECO:0000250}.
Tissue Specificity Isoform 1, isoform 3 and isoform 4 are expressed in brain. Isoform 1 is expressed in endocrine tissues. {ECO:0000269|PubMed:15028621, ECO:0000269|PubMed:9851558}.

Nischarin (D-1) - sc-374407 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nischarin (D-1): sc-374407. Western blot analysis of Nischarin expression in non-transfected: sc-117752 (A) and human Nischarin transfected: sc-116146 (B) 293T whole cell lysates.

Nischarin (B-3) - sc-365364 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nischarin (B-3): sc-365364. Western blot analysis of Nischarin expression in Hep G2 (A) and HeLa (B) whole cell lysates.

Nischarin (G-3) - sc-393568 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nischarin (G-3): sc-393568. Western blot analysis of Nischarin expression in PC-12 whole cell lysate.

Nischarin (H-300) - sc-98980 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Nischarin (H-300): sc-98980. Western blot analysis of Nischarin expression in non-transfected: sc-117752 (A) and human Nischarin transfected: sc-116146 (B) 293T whole cell lysates.

Nischarin (F-3) - sc-374408 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nischarin (F-3): sc-374408. Western blot analysis of Nischarin expression in non-transfected: sc-117752 (A) and human Nischarin transfected: sc-116146 (B) 293T whole cell lysates.

Nischarin (N-15) - sc-47239 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Nischarin (K-17) - sc-47238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nischarin (K-17): sc-47238. Western blot analysis of Nischarin expression in non-transfected: sc-117752 (A) and human Nischarin transfected: sc-116146 (B) 293T whole cell lysates.

Nischarin (C-16) - sc-47236 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nischarin (C-16): sc-47236. Western blot analysis of Nischarin expression in non-transfected: sc-117752 (A) and human Nischarin transfected: sc-116146 (B) 293T whole cell lysates.

Nischarin (K-15) - sc-47237 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nischarin (K-15): sc-47237. Western blot analysis of Nischarin expression in non-transfected: sc-117752 (A) and human Nischarin transfected: sc-116146 (B) 293T whole cell lysates.

Anti-NISCH antibody - ab56849 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ICC/IF image of ab56849 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56849, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NISCH Antibody (aa1335-1435) - LS-C314440 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant NISCH.

Anti-NISCH Antibody (aa1246-1346) - LS-C314437 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant NISCH.

Anti-NISCH Antibody (clone 3B8) - LS-C139385 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B8
Detection limit for recombinant GST tagged NISCH is 0.3 ng/ml as a capture antibody.

NISCH Antibody - NBP1-81630 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NISCH Antibody (2C8) - H00011188-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C8

NISCH Antibody (3B8) - H00011188-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B8
Sandwich ELISA: NISCH Antibody (3B8) [H00011188-M01] - Detection limit for recombinant GST tagged NISCH is 0.3 ng/ml as a capture antibody.

Monoclonal Anti-NISCH antibody produced in mouse - SAB1404914 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B8
<B>Immunoblotting</B><BR/>Monoclonal Anti-NISCH: <B>Cat. No. SAB1404914</B>: Antibody concentration: 1 μg/mL. Specific band of ~37.1 kDa using immunogen protein lysate.

Anti-NISCH antibody produced in rabbit - HPA023189 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NISCH: <B>Cat. No. HPA023189</B>: Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.

Monoclonal Anti-NISCH antibody produced in mouse - WH0011188M2 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C8
<B>Immunoblotting</B><BR/>Monoclonal Anti-NISCH, <B>Cat. No. WH0011188M2</B> used at the antibody concentration: 1 μg/mL. Specific band of ~37.11 kDa using immunogen protein lysate.

NISCH-Antibody-monoclonal-M02 - AT3052a from Abgent, a WuXi AppTec company

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C8
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .

NISCH antibody - 70R-18885 from Fitzgerald

Host Rabbit
Clonality Polyclonal

Nischarin antibody - 13813-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13813-1-AP(NISCH antibody) at dilution of 1:300

Nischarin antibody - H00011188-M01 from Acris Antibodies

Prices $360.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B8

Nischarin antibody - H00011188-M02 from Acris Antibodies

Prices $360.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C8
Detection limit for recombinant GST tagged NISCH is approximately 0.3ng/ml as a capture antibody.

Mouse Anti-Human NISCH - 101-11110 from RayBiotech

Host Mouse
See description below for image caption
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