EGLN3

Gene Symbol EGLN3
Entrez Gene 112399
Alt Symbol HIFP4H3, HIFPH3, PHD3
Species Human
Gene Type protein-coding
Description egl-9 family hypoxia-inducible factor 3
Other Description HIF prolyl hydroxylase 3|HIF-PH3|HIF-prolyl hydroxylase 3|HPH-1|HPH-3|egl nine homolog 3|egl nine-like protein 3 isoform|hypoxia-inducible factor prolyl hydroxylase 3|prolyl hydroxylase domain-containing protein 3
Swissprots Q3B8N4 Q9H6Z9 Q6P1R2 Q2TA79
Accessions EAW65929 Q9H6Z9 AJ310545 CAC42511 AK025273 BAB15101 AK026918 AK123350 BAG53892 AK225473 AK296216 BAG58941 BC010992 AAH10992 BC064924 AAH64924 BC102030 AAI02031 BC105938 AAI05939 BC105939 AAI05940 BC111057 AAI11058 BG716229 BX091357 BX346941 BX354108 CR592368 DQ975379 ABM44412 XM_006720015 XP_006720078 NM_001308103 NP_001295032 NM_022073 NP_071356
Function Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limi
Subcellular Location Nucleus. Cytoplasm. Note=Colocalizes with WDR83 in the cytoplasm. {ECO:0000250}.
Tissue Specificity Widely expressed at low levels. Expressed at higher levels in adult heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle), lung and placenta, and in fetal spleen, heart and skeletal muscle. Also expressed in pancreas. Localized to pancreatic acini and islet cells. {ECO:0000269|PubMed:12163023, ECO:0000269|PubMed:12670503, ECO:0000269|PubMed:21575608}.
Top Pathways Renal cell carcinoma

HIF PHD (S-20) - sc-46031 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HIF PHD (S-20): sc-46031. Western blot analysis of HIF PHD3 expression in non-transfected: sc-117752 (A) and mouse HIF PHD3 transfected: sc-122532 (B) 293T whole cell lysates.

HIF PHD3 (P-16) - sc-46030 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HIF PHD3 (P-16): sc-46030. Western blot analysis of HIF PHD3 expression in non-transfected: sc-117752 (A) and mouse HIF PHD3 transfected: sc-122532 (B) 293T whole cell lysates.

Anti-PHD3 antibody - ab30782 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PHD3 antibody (ab30782) at 1 µg/mlLane 1 : Hela-Vehicle treated (Negative Control) Whole Cell Lysate (ab116321)Lane 2 : Hela-DFO treated (0.5mM, 24h) Whole Cell Lysate (ab116322)Lane 3 : Human HeLa Nuclear DFO treatedLane 4 : Human HeLa Cytoplasmic DFO treatedLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PHD3 antibody - ab77397 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-PHD3 antibody (ab77397) at 0.1 µg/ml + Human Muscle lysate (in RIPA buffer) at 35 µg

Anti-PHD3 antibody - ab196344 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PHD3 antibody - ab88582 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PHD3 antibody (ab88582) at 1 µg + human placenta tissue lysate at 50 µgSecondaryGoat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

Anti-PHD3 antibody - ab123289 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PHD3 antibody (ab123289) at 1/100 dilution + HL60 cell line lysates at 35 µgdeveloped using the ECL technique

Anti-PHD3 antibody [EG188e/d5] - ab77610 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone EG188e/d5

Anti-EGLN3 / PHD3 Antibody (aa50-100) IHC-plus™ - LS-B11293 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-PHD3 / EGLN3 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11293 concentration 5 ug/ml.

Anti-EGLN3 / PHD3 Antibody (C-Terminus) IHC-plus™ - LS-B11948 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-EGLN3 / PHD3 Antibody (aa95-108) - LS-C3376 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (Internal) - LS-C9581 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (C-Terminus, Biotin) - LS-C141088 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (C-Terminus, DY650) - LS-C141089 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (C-Terminus, DY488) - LS-C141090 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (C-Terminus, HRP) - LS-C141091 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (C-Terminus, DY550) - LS-C141092 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (aa50-100, Biotin) - LS-C142752 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (aa50-100, DY650) - LS-C142753 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (aa50-100, DY488) - LS-C142754 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (aa50-100, HRP) - LS-C142755 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (aa108-119) - LS-C54756 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.1 ug/ml) staining of Human Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EGLN3 / PHD3 Antibody (aa50-100, DY550) - LS-C142756 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EGLN3 / PHD3 Antibody (clone A1.10, DY488) - LS-C143114 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone A1.10

Anti-EGLN3 / PHD3 Antibody (clone A1.10, DY650) - LS-C143113 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone A1.10
Search more