FBXO32

Gene Symbol FBXO32
Entrez Gene 114907
Alt Symbol Fbx32, MAFbx
Species Human
Gene Type protein-coding
Description F-box protein 32
Other Description F-box only protein 32|atrogin 1|atrogin-1|muscle atrophy F-box protein
Swissprots Q969P5 A4KYM0
Accessions EAW92040 EAW92041 EAW92042 EAW92043 Q969P5 AI077844 AJ420108 CAD12251 AK023391 AK056986 BAB71333 AK096904 AK124390 AK125888 AY059629 AAL16407 BC022416 BC024030 AAH24030 BC100020 AAI00021 BC120963 AAI20964 BC120964 AAI20965 DB503484 EF143260 ABO37797 HQ447898 ADQ32382 NM_001242463 NP_001229392 NM_058229 NP_478136 NM_148177 NP_680482
Function Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. {ECO:0000269|PubMed:15531760}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:22249105}. Nucleus {ECO:0000269|PubMed:22249105}. Note=Shuttles between Cytoplasm and the nucleus.
Tissue Specificity Specifically expressed in cardiac and skeletal muscle.

MAFbx (F-9) - sc-166806 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MAFbx (F-9): sc-166806. Western blot analysis of MAFbx expression in non-transfected: sc-117752 (A) and mouse MAFbx transfected: sc-121485 (B) 293T whole cell lysates.

MAFbx (H-300) - sc-33782 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MAFbx (H-300): sc-33782. Western blot analysis of MAFbx expression in non-transfected: sc-117752 (A) and mouse MAFbx transfected: sc-121485 (B) 293T whole cell lysates.

MAFbx (L-15) - sc-27645 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAFbx (L-15): sc-27645. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.

MAFbx (E-14) - sc-27644 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MAFbx (E-14): sc-27644. Western blot analysis of MAFbx expression in A10 (A) and HeLa (B) whole cell lysates.

Anti-Fbx32 antibody - ab67866 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Fbx32 antibody (ab67866) at 1/500 dilutionLane 1 : Fbx32 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-Fbx32 antibody [EPR9148(2)] - ab168372 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9148(2)
All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified)Lane 1 : Mouse muscleLane 2 : Rat muscleLysates/proteins at 20 µg per lane.

Anti-Fbx32 antibody [EPR9148(2)] (Alexa Fluor® 488) - ab198957 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9148(2)
ab198957 staining Fbx32 in A673 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198957 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Fbx32 antibody [EPR9148(2)] (Alexa Fluor® 647) - ab198959 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9148(2)
ab198959 staining Fbx32 in A673 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198959 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Fbx32 antibody [EPR9148(2)] (HRP) - ab198958 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9148(2)
All lanes : Anti-Fbx32 antibody [EPR9148(2)] (HRP) (ab198958) at 1/5000 dilutionLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Skeletal Muscle (Mouse) Tissue LysateLane 3 : Skeletal Muscle (Rat) Tissue LysateLane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 5 : Heart (Mouse) Tissue LysateLane 6 : Heart (Rat) Tissue LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-FBXO32 / Fbx32 Antibody - LS-C153194 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FBXO32 / Fbx32 Antibody - LS-C183433 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FBXO32 / Fbx32 Antibody - LS-C307970 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-FBXO32 / Fbx32 Antibody - LS-C307971 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-FBXO32 / Fbx32 Antibody - LS-C341487 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FBXO32 / Fbx32 Antibody - LS-C341761 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FBXO32 / Fbx32 Antibody (aa70-82) - LS-C112792 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
FBXO32 antibody (0.1 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-FBXO32 / Fbx32 Antibody - LS-C334959 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of Mouse heart cell lines, using FBXO32 antibody.

Anti-FBXO32 / Fbx32 Antibody - LS-C335272 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human colon cancer tissue using FBXO32 antibody.

Anti-FBXO32 / Fbx32 Antibody (C-Terminus) IHC-plus™ - LS-B9748 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-FBXO32 / MAFBX / Atrogin 1 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9748 concentration 5 ug/ml.

Fbx32 Antibody - NBP1-36955 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: Fbx32 Antibody [NBP1-36955] - (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Fbx32 Antibody - H00114907-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: Fbx32 Antibody [H00114907-B01P] - Analysis of FBXO32 expression in transfected 293T cell line by FBXO32 polyclonal antibody. Lane 1: FBXO32 transfected lysate(39.05 KDa). Lane 2: Non-transfected lysate.

Fbx32 Antibody (1D6) - H00114907-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D6

Fbx32 Polyclonal Antibody - bs-2591R from Bioss

Prices $279.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat uterus labeled with Anti MAFbx/Fbx32 Polyclonal Antibody, Unconjugated (bs2591R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Anti-FBXO32 antibody produced in goat - SAB2501208 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-FBXO32: <B>Cat. No. SAB2501208</B>: (0.1 μg/mL) staining of Human Skeletal Muscle lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Monoclonal Anti-FBXO32 antibody produced in mouse - SAB1403439 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1D6
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