Gene Symbol | PRPF8 |
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Entrez Gene | 10594 |
Alt Symbol | HPRP8, PRP8, PRPC8, RP13, SNRNP220 |
Species | Human |
Gene Type | protein-coding |
Description | pre-mRNA processing factor 8 |
Other Description | 220 kDa U5 snRNP-specific protein|PRP8 homolog|PRP8 pre-mRNA processing factor 8 homolog|U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p|apoptosis-regulated protein 1|apoptosis-regulated protein 2|p220|pre-mRNA-processing-splicing factor 8|precursor mRNA processing protein|splicing factor Prp8 |
Swissprots | Q6P2Q9 O75965 O14547 |
Accessions | EAW90588 EAW90589 EAW90590 EAW90591 EAW90592 EAW90593 EAW90594 Q6P2Q9 AB007510 BAA22563 AF092565 AAC61776 AK058034 AK126169 AK222905 BAD96625 AK296344 BAG59028 AY486134 AAS64747 AY486135 AAS64748 BC000579 BC034545 BC064370 AAH64370 BX649099 CD698473 DB081408 NM_006445 NP_006436 |
Function | Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. {ECO:0000269|PubMed:20595234, ECO:0000303|PubMed:15840809}. |
Subcellular Location | Nucleus speckle {ECO:0000250}. |
Tissue Specificity | Widely expressed. {ECO:0000269|PubMed:10411133}. |
Top Pathways | Spliceosome |
PRP8 CRISPR/Cas9 KO Plasmid (h) - sc-402819 from Santa Cruz Biotechnology
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PRP8 HDR Plasmid (h) - sc-402819-HDR from Santa Cruz Biotechnology
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PRP8 Double Nickase Plasmid (h) - sc-402819-NIC from Santa Cruz Biotechnology
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PRP8 Double Nickase Plasmid (h2) - sc-402819-NIC-2 from Santa Cruz Biotechnology
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PRP8 CRISPR Activation Plasmid (h) - sc-402819-ACT from Santa Cruz Biotechnology
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PRP8 CRISPR Activation Plasmid (h2) - sc-402819-ACT-2 from Santa Cruz Biotechnology
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PRP8 Lentiviral Activation Particles (h) - sc-402819-LAC from Santa Cruz Biotechnology
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PRP8 Lentiviral Activation Particles (h2) - sc-402819-LAC-2 from Santa Cruz Biotechnology
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PRP8 siRNA (h) - sc-38209 from Santa Cruz Biotechnology
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PRP8 shRNA Plasmid (h) - sc-38209-SH from Santa Cruz Biotechnology
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PRP8 shRNA (h) Lentiviral Particles - sc-38209-V from Santa Cruz Biotechnology
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PRPF8 RNAi - H00010594-R01 from Novus Biologicals
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MISSION® esiRNA - EHU087301 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17481 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17481 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR01341 from Sigma-Aldrich
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PRPF8 siRNA (Human) - CRH7211 from Cohesion Biosciences
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PRP8 CRISPR/Cas9 KO Plasmid (h2) - sc-402819-KO-2 from Santa Cruz Biotechnology
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PRP8 HDR Plasmid (h2) - sc-402819-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human PRPF8 (NM_006445.3) - SHH383832 from Creative biogene
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shRNA set against Human PRPF8(NM_006445.3) - SHR142862 from Creative biogene
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PRPF8 shRNA Plasmid (Human) - CHH7211 from Cohesion Biosciences
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PRPF8 miRNA 3'UTR clone - MiUTR1H-08320 from Creative biogene
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