Il1b

Gene Symbol	Il1b
Entrez Gene	16176
Alt Symbol	IL-1beta, Il-1b
Species	Mouse
Gene Type	protein-coding
Description	interleukin 1 beta
Other Description	IL-1 beta\|interleukin-1 beta
Swissprots	P10749 Q2M4J6
Accessions	AAX90604 CAA28637 EDL28238 P10749 AK156396 AK157245 AK168047 BAE40029 AK224980 AK225002 AK225003 BC011437 AAH11437 M15131 AAA39276 XM_006498795 XP_006498858 NM_008361 NP_032387
Function	Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Subcellular Location	Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Top Pathways	Cytosolic DNA-sensing pathway, Apoptosis, Inflammatory bowel disease (IBD), Inflammatory mediator regulation of TRP channels, Toll-like receptor signaling pathway

IL-1β (D3H1Z) Rabbit mAb (Mouse Specific) - 12507 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D3H1Z

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 μg/ml, overnight; +), using IL-1β (D3H1Z) Rabbit mAb (Mouse Specific).

IL-1β (D4T2D) Rabbit mAb (Mouse Specific) - 12426 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D4T2D

Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 μg/ml, overnight; +), using IL-1β (D4T2D) Rabbit mAb (Mouse Specific).

IL-1β Antibody (Mouse Specific) - 8689 from Cell Signaling Technology

Host	Rabbit
Clonality	Polyclonal

IL-1β (M-20) - sc-1251 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Western blot analysis of human recombinant IL-1β (A) and mouse recombinant IL-1β (B,C). Antibodies tested include IL-1β (C-20): sc-1250 (A), IL-1β (M-20): sc-1251 (B) and IL-1β (R-20): sc-1252 (C).

IL-1β (R-20) - sc-1252 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

cleaved IL-1β (m118) - sc-23460 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-IL1 beta antibody - ab193852 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-IL1 beta antibody [1400.24.17] - ab13784 from Abcam

Prices	$395.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	1400.24.17

Anti-IL1 beta antibody (Biotin) - ab106034 from Abcam

Prices	$373.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-IL1 beta antibody (HRP) - ab106035 from Abcam

Prices	$373.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-IL1B antibody - ab150777 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-IL1B antibody [17H18L16] - ab200478 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Monoclonal
Clone	17H18L16

Anti-IL1B antibody [17H18L16] (ab200478) at 2 µg/ml + Mouse IL1B recombinant proteindeveloped using the ECL technique

IL-1 beta/IL-1F2 Antibody [Unconjugated] - AF-401-NA from Novus Biologicals

Prices	$99.00, $369.00
Sizes	25 µg, 100 µg
Host	Goat
Clonality	Polyclonal

Recombinant MouseIL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-401-NA). The ND50 is typically ≤0.25 µg/mL.

IL-1 beta/IL-1F2 Antibody - NBP1-42767 from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.

IL-1 beta/IL-1F2 Antibody (30311) [Unconjugated] - MAB401 from Novus Biologicals

Prices	$99.00, $269.00, $699.00
Sizes	25 µg, 100 µg, 500 µg
Host	Rat
Clonality	Monoclonal
Clone	30311

IL-1 beta/IL-1F2 Antibody [Unconjugated] - AB-401-NA from Novus Biologicals

Prices	$359.00
Sizes	1 mg
Host	Goat
Clonality	Polyclonal

Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 40 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse IL‑1 beta /IL‑1F2 Polyclonal Antibody (Catalog # AB-401-NA). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

IL-1 beta/IL-1F2 Antibody (B122) - MAB4012 from Novus Biologicals

Prices	$99.00, $259.00
Sizes	25 µg, 100 µg
Host	Hamster
Clonality	Monoclonal
Clone	B122

IL-1 beta/IL-1F2 Antibody (166926) [Unconjugated] - MAB4011 from Novus Biologicals

Prices	$99.00, $269.00, $699.00
Sizes	25 µg, 100 µg, 500 µg
Host	Rat
Clonality	Monoclonal
Clone	166926

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB4011) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL-1 beta/IL-1F2 Antibody (166931) - MAB4013 from Novus Biologicals

Prices	$249.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	166931

Intracellular Staining by Flow Cytometry IL-1 beta/IL-1F2 Antibody (166931)

IL-1 beta/IL-1F2 Antibody (RM0369-7A36) - NBP2-32729 from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	RM0369-7A36

IL-1 beta/IL-1F2 Antibody [Biotin] - BAF401 from Novus Biologicals

Prices	$420.00
Sizes	50 µg
Host	Goat
Clonality	Polyclonal

IL-1 beta/IL-1F2 Antibody (1400.24.17) - NBP2-31154 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1400.24.17

IL-1 beta/IL-1F2 Antibody (166931) [Allophycocyanin] - IC4013A from Novus Biologicals

Prices	$339.00
Sizes	100 tests
Host	Rat
Clonality	Monoclonal
Clone	166931

Mouse splenocytes were stained with Rat Anti-Mouse IL-1 beta /IL-1F2 APC-conjugated Monoclonal Antibody (Catalog # IC4013A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

IL-1 beta/IL-1F2 Antibody (166931) [Phycoerythrin] - IC4013P from Novus Biologicals

Prices	$289.00
Sizes	100 tests
Host	Rat
Clonality	Monoclonal
Clone	166931

Mouse splenocytes either untreated (open histogram) or treated with LPS for 48 hours (filled histogram) were stained with Rat Anti-Mouse IL-1 beta /IL-1F2 PE-conjugated Monoclonal Antibody (Catalog # IC4013P) or isotype control antibody (Catalog # IC013P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

IL-1 beta/IL-1F2 Antibody (166931) [Fluorescein] - IC4013F from Novus Biologicals

Prices	$239.00
Sizes	100 tests
Host	Rat
Clonality	Monoclonal
Clone	166931

Mouse splenocytes, untreated (open histogram), or treated with LPS for 48 hours (filled histogram) were stained with Rat Anti-Mouse IL-1 beta /IL-1F2 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC4013F, filled histogram) or isotype control antibody (Catalog # IC013F, data not shown). Histograms were gated on blasting splenocytes. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Search more