| Gene Symbol | WHAMM |
|---|---|
| Entrez Gene | 123720 |
| Alt Symbol | WHDC1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | WAS protein homolog associated with actin, golgi membranes and microtubules |
| Other Description | WAS protein homology region 2 domain-containing protein 1|WASP homolog-associated protein with actin, membranes and microtubules|WH2 domain-containing protein 1 |
| Swissprots | Q8N1J9 Q8TF30 |
| Accessions | EAW62438 Q8TF30 AB075851 BAB85557 AI377476 AK097947 BAC05201 AK126887 BC167774 AAI67774 XM_005272421 XP_005272478 XM_005272422 XP_005272479 XM_005272423 XP_005272480 XM_005272424 XP_005272481 XM_011521232 XP_011519534 XM_011521233 XP_011519535 NM_001080435 NP_001073904 |
| Function | Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Its activity in membrane tubulation requires F-actin and interaction with microtubules. Proposed to use coordinated actin-nucleating and microtubule- binding activities of distinct WHAMM molecules to drive membrane tubule elongation; when MT-bound can recruit and remodel membrane vesicles but is prevented to activate the Arp2/3 complex. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex. Required for RhoD-dependent actin reorganization such as in cell adhesion and cell migration. {ECO:0000269|PubMed:18614018, ECO:0000269|PubMed:23027905, ECO:0000269|PubMed:23087206}. |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:18614018}. Endoplasmic reticulum-Golgi intermediate compartment {ECO:0000269|PubMed:18614018}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:18614018}. Golgi apparatus, cis-Golgi network {ECO:0000269|PubMed:18614018}. Note=Localized to a perinuclear compartment near the microtubule-organizing center (MTOC). Also detected on tubulo-vesicular structures in the cell periphery that frequently localized along microtubules. {ECO:0000269|PubMed:18614018}. |
| Tissue Specificity | Expressed in brain, lung, heart, colon and kidney (at protein level). |
WHDC1 CRISPR/Cas9 KO Plasmid (h) - sc-408507 from Santa Cruz Biotechnology
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WHDC1 HDR Plasmid (h) - sc-408507-HDR from Santa Cruz Biotechnology
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WHDC1 Double Nickase Plasmid (h) - sc-408507-NIC from Santa Cruz Biotechnology
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WHDC1 Double Nickase Plasmid (h2) - sc-408507-NIC-2 from Santa Cruz Biotechnology
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WHDC1 CRISPR Activation Plasmid (h) - sc-408507-ACT from Santa Cruz Biotechnology
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WHDC1 CRISPR Activation Plasmid (h2) - sc-408507-ACT-2 from Santa Cruz Biotechnology
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WHDC1 Lentiviral Activation Particles (h) - sc-408507-LAC from Santa Cruz Biotechnology
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WHDC1 Lentiviral Activation Particles (h2) - sc-408507-LAC-2 from Santa Cruz Biotechnology
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WHDC1 siRNA (h) - sc-90314 from Santa Cruz Biotechnology
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WHDC1 shRNA Plasmid (h) - sc-90314-SH from Santa Cruz Biotechnology
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WHDC1 shRNA (h) Lentiviral Particles - sc-90314-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU127381 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22892 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22892 from Sigma-Aldrich
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WHAMM siRNA (Human) - CRJ4737 from Cohesion Biosciences
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WHAMM - MBS8238909 from MyBioSource
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shRNA set against Human WHAMM (NM_001080435.1) - SHH446956 from Creative biogene
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shRNA set against Human WHDC1(NM_001080435.1) - SHL132218 from Creative biogene
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Human WHAMM siRNA - orb275238 from Biorbyt
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WHAMM shRNA Plasmid (Human) - CHJ4737 from Cohesion Biosciences
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WHAMM miRNA 3'UTR clone - MiUTR1H-11258 from Creative biogene
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