BRAF

Gene Symbol BRAF
Entrez Gene 673
Alt Symbol B-RAF1, BRAF1, NS7, RAFB1
Species Human
Gene Type protein-coding
Description B-Raf proto-oncogene, serine/threonine kinase
Other Description 94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|serine/threonine-protein kinase B-raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1
Swissprots P15056 Q9UDP8 Q13878 Q9Y6T3 Q3MIN6 A4D1T4
Accessions AAD15551 AAD43193 AAS00359 ACD11489 ADQ00186 ADX94397 AIE38317 CAA46301 CAZ68014 CBK51920 EAL24023 EAW83964 EAW83965 P15056 AA252358 AA834931 AI203435 AK054801 AK094023 AK098095 AK124257 AK314346 AW971583 BC038966 BC101757 AAI01758 BC112079 AAI12080 BM263585 DB454480 DN996440 HM437233 ADN43065 M21001 AAA96495 M95712 AAA35609 XM_005250045 XP_005250102 XM_005250046 XP_005250103 XM_011516529 XP_011514831 XM_011516530 XP_011514832 XR_242190 XR_927520 XR_927521 XR_927522 XR_927523 NM_004333 NP_004324
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. {ECO:0000269|PubMed:21441910}.
Subcellular Location Nucleus {ECO:0000250}. Cytoplasm. Cell membrane {ECO:0000250}. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. {ECO:0000250}.
Tissue Specificity Brain and testis.
Top Pathways Chemokine signaling pathway, Vascular smooth muscle contraction, Hepatitis C, Neurotrophin signaling pathway, Alcoholism

Phospho-B-Raf (Ser445) Antibody - 2696 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Raji and HeLa cells treated with TPA (200nM, 30 minutes) using Phospho-B-Raf (Ser445) Antibody (top) and total B-Raf Antibody (bottom).

B-Raf (55C6) Rabbit mAb - 9433 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 55C6
Western blot analysis of extracts from HeLa, C2C12 and NBT-II cells, using B-Raf (55C6) Rabbit mAb.

B-Raf (L12G7) Mouse mAb - 9434 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone L12G7
Western blot analysis of extracts from K-562, HeLa, C1C12, NBT-II and COS cells, using B-Raf (L12G7) Mouse mAb.

Raf-B (C-19) - sc-166 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Raf-B (C-19)-G: sc-166-G. Western blot analysis of Raf-B expression in PC-12 whole cell lysate.

Raf-B (F-3) - sc-55522 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Raf-B (F-3): sc-55522. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Raf-B (H-145) - sc-9002 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Raf-B (H-145): sc-9002. Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.

Raf-B (F-7) - sc-5284 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Raf-B (F-7): sc-5284. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

Raf-B (13) - sc-136263 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Raf-B (13): sc-136263. Western blot analysis of Raf-B expression in Jurkat whole cell lysate.

p-Raf-B (Thr 598/Ser 601) - sc-28006 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Raf-B (Thr 598/Ser 601): sc-28006. Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterus tissue showing cytoplasmic staining of glandular cells.

Anti-B Raf (mutated V600 E) antibody [K21-F] (FITC) - ab175637 from Abcam

Prices $370.00
Sizes 50 tests
Host Rabbit
Clonality Monoclonal
Clone K21-F
Flow cytometric analysis of permeabilized Human peripheral blood lymphocytes labeling B Raf with ab175637 at 10μl/1 x 106 cells (blue), compared to an isotype control (black).

Anti-B Raf (phospho S446) antibody - ab76200 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-B Raf (phospho S446) antibody (ab76200) at 1/1000 dilutionLane 1 : Human Jurkat cells treated with calyculin A (100 nM) for 30 min, untreatedLane 2 : Human Jurkat cells treated with calyculin A (100 nM) for 30 min, treated with lambda phosphatase

Anti-B Raf (phospho S601) antibody - ab60997 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Human lymph node tissue stained for phosphorilated B Raf with ab60997 at 1/50-1/100 dilution, in the presence (right) or absence (left) of the immunizing phosphopeptide.

Anti-B Raf (phospho S729) antibody [EPR2207] - ab124794 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2207
All lanes : Anti-B Raf (phospho S729) antibody [EPR2207] (ab124794) at 1/1000 dilutionLane 1 : PC-12 cell lysates (untreated)Lane 2 : PC-12 cell lysates treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-B Raf (phospho T401) antibody [EPR2208Y] - ab68215 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2208Y
All lanes : Anti-B Raf (phospho T401) antibody [EPR2208Y] (ab68215) at 1/5000 dilutionLane 1 : PC-12 cell lysates (cells were untreated)Lane 2 : PC-12 cell lysates (cells were treated with TPA) Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-B Raf (phospho T598 + S601) antibody - ab115267 from Abcam

Prices $407.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab115267, at a 1/100 dilution, staining Raf in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, phosphatase-streptavidin and chromogen.

Anti-B Raf (phospho T598) antibody - ab59406 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59406 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.

Anti-B Raf (phospho T599) antibody - ab192813 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling B Raf (phospho T599) using ab192813 at a 1/50 dilution, the right panel was treated with blocking peptide.

Anti-B Raf (phospho T753) antibody - ab138399 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-B Raf (phospho T753) antibody (ab138399) at 1/500 dilution + K562 cell extracts, treated with 200ng/ml EGF (30 minutes) at 30 µg

Anti-B Raf antibody - ab65050 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab65050 at 1/50 dilution staining B Raf in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-B Raf antibody - ab85972 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling B Raf with ab85972 at 1/200 (1µg/ml). Detection: DAB.

Anti-B Raf antibody - ab59354 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59354, at a 1/500-1/100 dilution, staining human B Raf in breast carcinoma with (+) or without (-) immunising peptide, using Immunohistochemistry, Paraffin embedded tissue

Anti-B Raf antibody - ab196882 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-B Raf antibody (ab196882) at 1/500 dilutionLane 1 : Extract from 293 cells treated with insulin (0.01 U/ml, 15 minutes)Lane 2 : Extract from 293 cells treated with insulin (0.01 U/ml, 15 minutes) with Synthesized peptide

Anti-B Raf antibody - ab40899 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-B Raf antibody (ab40899) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Rat) Rat Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-B Raf antibody - ab59357 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-B Raf antibody (ab59357) at 1/500 dilutionLane 1 : Extracts from K562 cells, minus immunising peptideLane 2 : Extracts from K562 cells, plus immunising peptideObserved band size : 85 kDa (why is the actual band size different from the predicted?)

Anti-B Raf antibody - ab196901 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-B Raf antibody (ab196901) at 1/500 dilutionLane 1 : Rat brain cell lysateLane 2 : Rat brain cell lysate with synthesized peptide
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