TUT1
| Gene Symbol | TUT1 |
|---|---|
| Entrez Gene | 64852 |
| Alt Symbol | PAPD2, RBM21, STARPAP, TUTase, URLC6 |
| Species | Human |
| Gene Type | protein-coding |
| Description | terminal uridylyl transferase 1, U6 snRNA-specific |
| Other Description | PAP-associated domain-containing 2|RNA binding motif protein 21|RNA uridylyltransferase|RNA-binding motif protein 21|RNA-binding protein 21|TUTase 6|U6 TUTase|U6 snRNA-specific terminal uridylyltransferase 1|U6-TUTase|nuclear speckle targeted phosphatidylinositol 4-phosphate 5-kinase type I-alpha regulated-poly(A) polymerase|nuclear speckle-targeted PIPK1A-regulated-poly(A) polymerase|poly(A) polymerase associated domain containing 2|speckle targeted PIP5K1A-regulated poly(A) polymerase|star-PAP|up-regulated in lung cancer 6 |
| Swissprots | Q7L583 Q9H6E5 Q9H6H7 A1A527 Q2NL65 A8K995 |
| Accessions | EAW74029 Q9H6E5 AB101208 BAQ19752 AK025920 BAB15282 AK026000 BAB15314 AK292610 BAF85299 BC005013 AAH05013 BC110910 AAI10911 BC128263 AAI28264 CA444886 DQ586957 NM_022830 NP_073741 |
| Function | Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication- dependent histone mRNA degradation. {ECO:0000269|PubMed:16790842, ECO:0000269|PubMed:18288197, ECO:0000269|PubMed:21102410}. |
| Subcellular Location | Nucleus, nucleolus. Nucleus speckle. |
| Tissue Specificity | Widely expressed. {ECO:0000269|PubMed:18288197}. |
TUTase CRISPR/Cas9 KO Plasmid (h) - sc-406803 from Santa Cruz Biotechnology
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TUTase HDR Plasmid (h) - sc-406803-HDR from Santa Cruz Biotechnology
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TUTase Double Nickase Plasmid (h) - sc-406803-NIC from Santa Cruz Biotechnology
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TUTase Double Nickase Plasmid (h2) - sc-406803-NIC-2 from Santa Cruz Biotechnology
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TUTase CRISPR Activation Plasmid (h) - sc-406803-ACT from Santa Cruz Biotechnology
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TUTase CRISPR Activation Plasmid (h2) - sc-406803-ACT-2 from Santa Cruz Biotechnology
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TUTase Lentiviral Activation Particles (h) - sc-406803-LAC from Santa Cruz Biotechnology
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TUTase Lentiviral Activation Particles (h2) - sc-406803-LAC-2 from Santa Cruz Biotechnology
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TUTase siRNA (h) - sc-96738 from Santa Cruz Biotechnology
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TUTase shRNA Plasmid (h) - sc-96738-SH from Santa Cruz Biotechnology
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TUTase shRNA (h) Lentiviral Particles - sc-96738-V from Santa Cruz Biotechnology
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TUT1 RNAi - H00064852-R01 from Novus Biologicals
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MISSION® esiRNA - EHU078711 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22290 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22290 from Sigma-Aldrich
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TUT1 siRNA (Human) - CRJ2119 from Cohesion Biosciences
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TUT1 - MBS8234794 from MyBioSource
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TUTase CRISPR/Cas9 KO Plasmid (h2) - sc-406803-KO-2 from Santa Cruz Biotechnology
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TUTase HDR Plasmid (h2) - sc-406803-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human TUT1 (NM_022830.2) - SHH438024 from Creative biogene
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Human TUT1 siRNA - orb272620 from Biorbyt
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TUT1 shRNA Plasmid (Human) - CHJ2119 from Cohesion Biosciences
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TUT1 miRNA 3'UTR clone - MiUTR3H-11384 from Creative biogene
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