PCGF3

Gene Symbol	PCGF3
Entrez Gene	10336
Alt Symbol	DONG1, RNF3, RNF3A
Species	Human
Gene Type	protein-coding
Description	polycomb group ring finger 3
Other Description	RING finger protein 3A\|polycomb group RING finger protein 3\|ring finger protein 3
Swissprots	Q3KNV8 O15262 D3DVN1
Accessions	EAW82651 EAW82652 EAW82653 EAW82654 EAW82655 EAW82656 Q3KNV8 AA401257 AK057124 BAG51868 AK125801 BAG54250 AK225892 AK300290 BAG62047 AL832536 CAD38631 AU132917 BC028026 BC050044 BC061897 BC107061 AAI07062 BI758022 BQ882065 BX640894 BX648409 CA454695 CX761238 CX761943 XM_005272250 XP_005272307 XM_005272253 XP_005272310 XM_005272254 XP_005272311 XM_006713852 XP_006713915 XM_011513381 XP_011511683 XM_011513382 XP_011511684 XM_011513383 XP_011511685 XM_011513384 XP_011511686 XM_011513385 XP_011511687 NM_006315 NP_006306
Function	Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:21282530}.
Top Pathways	Signaling pathways regulating pluripotency of stem cells

PCGF3 (E-14) - sc-104578 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

PCGF3 (D-22) - sc-133892 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

PCGF3 (D-22): sc-133892. Western blot analysis of PCGF3 expression in non-transfected 293T: sc-117752 (A), human PCGF3 transfected 293T: sc-114793 (B) and HL-60 (C) whole cell lysates.

Anti-RNF3 (247 aa isoform) antibody - ab49248 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNF3 (247 aa isoform) antibody (ab49248) at 2.5 µg/ml + Fetal brain lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilutiondeveloped using the ECL technique

Anti-RNF3 (247 aa isoform) antibody - ab3892 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

ab3892 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-PCGF3 Antibody (clone 1F1) - LS-C134023 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F1

Immunoperoxidase of monoclonal antibody to PCGF3 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Anti-PCGF3 Antibody (aa103-152) - LS-C30649 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PCGF3 Antibody (aa96-145) - LS-C29934 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PCGF3 Antibody (aa235-247) - LS-C55404 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Antibody staining (1 ug/ml) of Human Heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

PCGF3 Antibody - NBP1-84062 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: PCGF3 Antibody [NBP1-84062] - Staining of human cell line U-2 OS shows positivity in mitochondria.

PCGF3 Antibody - H00010336-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: PCGF3 Antibody [H00010336-B01] - analysis of PCGF3 expression in transfected 293T cell line by PCGF3 MaxPab polyclonal antibody (B01). Lane 1: PCGF3 transfected lysate (28.10 KDa). Lane 2: Non-transfected lysate.

PCGF3 Antibody (1F1) - H00010336-M03 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F1

Immunohistochemistry-Paraffin: PCGF3 Antibody (1F1) [H00010336-M03] - Analysis of monoclonal antibody to PCGF3 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml

PCGF3 Antibody - NB100-1171 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Western Blot: PCGF3 Antibody [NB100-1171] - (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PCGF3 Antibody - H00010336-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

PCGF3 Polyclonal Antibody - bs-9169R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PCGF3 antibody produced in rabbit - HPA018487 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-PCGF3: <B>Cat. No. HPA018487</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon shows strong cytoplasmic positivity with a granular pattern in glandular cells.

Monoclonal Anti-PCGF3, (C-terminal) antibody produced in mouse - SAB1402512 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	1F1

<B>Immunohistochemistry</B><BR/>Monoclonal Anti-PCGF3, antibody produced in mouse, clone 1F1, purified immunoglobulin: <B>Cat. No. SAB1402512</B>: Antibody concentration: 3 μg/mL using human small intestine tissue.

Anti-PCGF3 (AB1) antibody produced in rabbit - AV34416 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: Raji (<B>Cat. No. AV34416</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.125 μg/mL in cell type Raji

Anti-PCGF3 (AB2) antibody produced in rabbit - AV34415 from Sigma-Aldrich

Prices	$267.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: fetal brain (<B>Cat. No. AV34415</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 2.5 μg/mL in cell type fetal brain

Anti-RNF3 (247 AA Isoform) antibody produced in goat - SAB2500888 from Sigma-Aldrich

Prices	$376.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-RNF3 (247 aa isoform): <B>Cat. No. SAB2500888</B>: staining (1 μg/mL) of Human Heart lysate (RIPA buffer, 30 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PCGF3 antibody - middle region (ARP34415_T100) - ARP34415_T100 from Aviva Systems Biology

Prices	$229.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

PCGF3 antibody - middle region (ARP34416_P050) - ARP34416_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

RNF3-247-aa-isoform-Antibody - AF1935a from Abgent, a WuXi AppTec company

Prices	$335.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AF1935a staining (1 µg/ml) of Human Heart lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PCGF3 Antibody (OAEB01505) - OAEB01505 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

PCGF3 Polyclonal Antibody - A7459 from ABclonal

Prices	$130.00, $220.00, $380.00
Sizes	50 µl, 100 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using PCGF3 antibody.

PCGF3 / RNF3 (247 aa isoform, C-term) antibody - AP16106PU-N from Acris Antibodies

Prices	$310.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AP16106PU-N staining (1µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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