CHST5

Gene Symbol CHST5
Entrez Gene 23563
Alt Symbol I-GlcNAc-6-ST, I-GlcNAc6ST, glcNAc6ST-3, gn6st-3, hIGn6ST
Species Human
Gene Type protein-coding
Description carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
Other Description GST4-alpha|N-acetylglucosamine 6-O-sulfotransferase 3|carbohydrate sulfotransferase 5|galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-alpha|intestinal GlcNAc-6-sulfotransferase
Swissprots Q9GZS9 B2RV23 Q9UBY3 Q7LCN3
Accessions AAD56001 AAG26326 EAW95635 EAW95636 Q9GZS9 AF246718 AAG28023 BC147002 AAI47003 BC147007 AAI47008 BX282540 HQ448069 ADQ32550 NM_024533 NP_078809 NM_012126
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location. {ECO:0000269|PubMed:10491328, ECO:0000269|PubMed:11352640, ECO:0000269|PubMed:12218059, ECO:0000269|PubMed:12626414}.
Subcellular Location Golgi apparatus membrane {ECO:0000305|PubMed:12855678}; Single-pass type II membrane protein {ECO:0000305|PubMed:12855678}. Note=Golgi membrane, early secretory pathway.
Tissue Specificity Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas. {ECO:0000269|PubMed:10491328, ECO:0000269|PubMed:11017086, ECO:0000269|PubMed:11352640, ECO:0000269|PubMed:12107080, ECO:0000269|PubMed:12626414}.

Anti-CHST5 antibody - ab118108 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-CHST5 antibody (ab118108) at 5 µg/ml + Immunogen at 0.2 µgdeveloped using the ECL technique

Anti-CHST5 antibody - C-terminal - ab139121 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CHST5 antibody - C-terminal (ab139121) at 1/100 dilution + ZR-75-1 cell line lysates at 35 µg

Anti-CHST5 Antibody (aa275-302, AP) - LS-C208451 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST5 Antibody (aa28-390) - LS-C125249 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-CHST5 Antibody (aa275-302, APC) - LS-C208452 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST5 Antibody (aa275-302, FITC) - LS-C208454 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST5 Antibody (aa275-302, Azide-free, HRP) - LS-C208455 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST5 Antibody (aa275-302, PE) - LS-C208456 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST5 Antibody (aa275-302, Biotin) - LS-C208453 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHST5 Antibody (aa310-391) - LS-C307798 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CHST5 Antibody (aa275-302) - LS-C159351 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
CHST5 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The CHST5 antibody detected the CHST5 protein (arrow).

Anti-CHST5 Antibody (aa275-302) - LS-C320385 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Carbohydrate Sulfotransferase 5/CHST5 Antibody - AF5446 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Carbohydrate Sulfotransferase 5/CHST5 Antibody - NBP1-91785 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Carbohydrate Sulfotransferase 5/CHST5 Antibody (2E6) - H00023563-M05 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E6
Sandwich ELISA: Carbohydrate Sulfotransferase 5/CHST5 Antibody (2E6) [H00023563-M05] - Detection limit for recombinant GST tagged CHST5 is 0.1 ng/ml as a capture antibody.

Monoclonal Anti-CHST5, (C-terminal) antibody produced in mouse - SAB1403151 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E6
<B>Immunoblotting</B><BR/>Monoclonal Anti-CHST5: <B>Cat. No. SAB1403151</B>: Antibody concentration: 1 μg/mL. Specific band of ~35 kDa using immunogen protein lysate.

CHST5-Antibody-C-term - AP18949b from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
CHST5 Antibody (C-term) (Cat. #AP18949b) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CHST5 antibody detected the CHST5 protein (arrow).

Chst5-mouse-Antibody-C-Term - AF2910a from Abgent, a WuXi AppTec company

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

CHST5/6 antibody [N1C3] - GTX110290 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-CHST5/6  antibody [N1C3] used in Western blot. GTX110290

CHST5 Antibody - C-terminal region (OAAB14487) - OAAB14487 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

Human Carbohydrate Sulfotransferase 5/CHST5 Antibody - AF5446 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Detection of Human Carbohydrate Sulfotransferase 5/CHST5 by Western blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019 ). A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .

Human CHST5 Affinity Purified Polyclonal Ab, Goat IgG - AF5446-SP from R&D Systems

Host Goat
Clonality Polyclonal
Detection of HumanCarbohydrate Sulfotransferase 5/CHST5 by Western blot.Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 5/CHST5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbohydrate Sulfotransferase 5/CHST5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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