Gene Symbol | CENPU |
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Entrez Gene | 79682 |
Alt Symbol | CENP50, CENPU50, KLIP1, MLF1IP, PBIP1 |
Species | Human |
Gene Type | protein-coding |
Description | centromere protein U |
Other Description | KSHV latent nuclear antigen interacting protein 1|KSHV latent nuclear antigen-interacting protein 1|MLF1 interacting protein|MLF1-interacting protein|centromere protein of 50 kDa|interphase centromere complex protein 24|polo-box-interacting protein 1 |
Swissprots | Q32Q71 A2RRD9 Q71F23 Q9H5G1 Q09GN2 |
Accessions | EAX04666 Q71F23 AA761728 AF469667 AAQ05290 AF516710 AAQ08228 AK027121 BAB15665 AK292297 BAF84986 AW440254 BC031520 AAH31520 BC067802 BC107744 AAI07745 BC131556 AAI31557 BC141854 AAI41855 BG031878 BX355581 CR457376 CAG33657 DQ907910 ABI49142 DQ907911 ABI49143 XM_005263218 XP_005263275 NM_024629 NP_078905 NR_104593 |
Function | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. {ECO:0000269|PubMed:12941884, ECO:0000269|PubMed:16716197, ECO:0000269|PubMed:17081991}. |
Subcellular Location | Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates. |
Tissue Specificity | Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines. {ECO:0000269|PubMed:12941884, ECO:0000269|PubMed:15116101, ECO:0000269|PubMed:15893739}. |
MLF1IP CRISPR/Cas9 KO Plasmid (h) - sc-413208 from Santa Cruz Biotechnology
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MLF1IP HDR Plasmid (h) - sc-413208-HDR from Santa Cruz Biotechnology
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MLF1IP Double Nickase Plasmid (h) - sc-413208-NIC from Santa Cruz Biotechnology
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MLF1IP Double Nickase Plasmid (h2) - sc-413208-NIC-2 from Santa Cruz Biotechnology
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MLF1IP CRISPR Activation Plasmid (h) - sc-413208-ACT from Santa Cruz Biotechnology
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MLF1IP CRISPR Activation Plasmid (h2) - sc-413208-ACT-2 from Santa Cruz Biotechnology
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MLF1IP Lentiviral Activation Particles (h) - sc-413208-LAC from Santa Cruz Biotechnology
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MLF1IP Lentiviral Activation Particles (h2) - sc-413208-LAC-2 from Santa Cruz Biotechnology
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MLF1IP siRNA (h) - sc-61057 from Santa Cruz Biotechnology
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MLF1IP shRNA Plasmid (h) - sc-61057-SH from Santa Cruz Biotechnology
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MLF1IP shRNA (h) Lentiviral Particles - sc-61057-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU021731 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN14393 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND14393 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08595 from Sigma-Aldrich
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CENPU siRNA (Human) - CRJ2483 from Cohesion Biosciences
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CENPU - MBS8238434 from MyBioSource
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shRNA set against Human MLF1IP (NM_024629.3) - SHH342440 from Creative biogene
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CENPU shRNA Plasmid (Human) - CHJ2483 from Cohesion Biosciences
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MLF1IP miRNA 3'UTR clone - MiUTR3H-11943 from Creative biogene
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