Gene Symbol | AMER1 |
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Entrez Gene | 139285 |
Alt Symbol | FAM123B, OSCS, WTX |
Species | Human |
Gene Type | protein-coding |
Description | APC membrane recruitment protein 1 |
Other Description | RP11-403E24.2|Wilms tumor gene on the X chromosome protein|Wilms tumor on the X|adenomatous polyposis coli membrane recruitment 1|family with sequence similarity 123B|protein FAM123B |
Swissprots | Q5JTC6 Q8N885 A2IB86 |
Accessions | EAX05415 Q5JTC6 AK097146 BAC04964 AL833759 DA939151 EF186024 ABM60755 XM_011530858 XP_011529160 NM_152424 NP_689637 |
Function | Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta- catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development. {ECO:0000269|PubMed:17510365, ECO:0000269|PubMed:17925383, ECO:0000269|PubMed:19416806, ECO:0000269|PubMed:21304492, ECO:0000269|PubMed:21498506}. |
Subcellular Location | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Translocates to the cell membrane following binding to PtdIns(4,5)P2. |
Tissue Specificity | Detected in fetal and adult kidney, brain and spleen. {ECO:0000269|PubMed:19416806}. |
WTX CRISPR/Cas9 KO Plasmid (h) - sc-409039 from Santa Cruz Biotechnology
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WTX HDR Plasmid (h) - sc-409039-HDR from Santa Cruz Biotechnology
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WTX Double Nickase Plasmid (h) - sc-409039-NIC from Santa Cruz Biotechnology
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WTX Double Nickase Plasmid (h2) - sc-409039-NIC-2 from Santa Cruz Biotechnology
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WTX CRISPR Activation Plasmid (h) - sc-409039-ACT from Santa Cruz Biotechnology
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WTX CRISPR Activation Plasmid (h2) - sc-409039-ACT-2 from Santa Cruz Biotechnology
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WTX Lentiviral Activation Particles (h) - sc-409039-LAC from Santa Cruz Biotechnology
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WTX Lentiviral Activation Particles (h2) - sc-409039-LAC-2 from Santa Cruz Biotechnology
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WTX siRNA (h) - sc-91345 from Santa Cruz Biotechnology
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WTX shRNA Plasmid (h) - sc-91345-SH from Santa Cruz Biotechnology
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WTX shRNA (h) Lentiviral Particles - sc-91345-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN8150 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND8150 from Sigma-Aldrich
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AMER1 siRNA (Human) - CRJ5249 from Cohesion Biosciences
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AMER1 - MBS8203236 from MyBioSource
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shRNA set against Human FAM123B (NM_152424.3) - SHH289377 from Creative biogene
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Human AMER1 siRNA - orb275750 from Biorbyt
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AMER1 shRNA Plasmid (Human) - CHJ5249 from Cohesion Biosciences
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FAM123B miRNA 3'UTR clone - MiUTR1H-03410 from Creative biogene
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